公开(公告)号：WO2008076916A2

公开(公告)日：2008-06-26

申请号：PCT/US2007/087629

申请日：2007-12-14

Applicant: MOLECULAR DEVICES CORPORATION ,  BHATTACHARYYA, Sukanta ,  SPORTSMAN, John Richard

Inventor： BHATTACHARYYA, Sukanta ,  SPORTSMAN, John Richard

IPC: C07D409/02

CPC classification number: C07D405/04 ,  C07D407/04 ,  C07D409/04 ,  C07D409/14 ,  C07D421/04

Abstract: The present application provides novel thallium-sensitive agents and assays using those agents, e.g., for identifying modulators of ion channels, channel-linked receptors or ion transporters.

Abstract translation: 本申请提供了新颖的铊敏感试剂和使用这些试剂的试验，例如鉴定离子通道调节剂，通道连接受体或离子转运蛋白。

公开(公告)号：WO1996003637A1

公开(公告)日：1996-02-08

申请号：PCT/US1995009459

申请日：1995-07-25

Applicant: MOLECULAR DEVICES CORPORATION

Inventor： MOLECULAR DEVICES CORPORATION ,  HAFEMAN, Dean, G.

IPC: G01N21/25

CPC classification number: G01N21/253 ,  G01N21/3151

Abstract: Disclosed are photometric methods and devices for determining optical pathlength of liquid samples containing analytes dissolved or suspended in a solvent. The methods and devices rely on determining a relationship between the light absorption properties of the solvent and the optical pathlength of liquid samples containing the solvent. This relationship is used to establish the optical pathlength for samples containing an unknown concentration of analyte but having similar solvent composition. Further disclosed are methods and devices for determining the concentration of analyte in such samples where both the optical pathlength and the concentration of analyte are unknown. The methods and devices rely on separately determining, at different wavelengths of light, light absorption by the solvent and light absorption by the analyte. Light absorption by the analyte, together with the optical pathlength so determined, is used to calculate the concentration of the analyte. Devices for carrying out the methods particularly advantageously include vertical-beam photometers containing samples disposed within the wells of multi-assays plates, wherein the photometer is able to monitor light absorption of each sample at multiple wavelengths, including in the visible or UV-visible region of the spectrum, as well as in the near-infrared region of the electromagnetic spectrum. Novel photometer devices are described which automatically determine the concentration of analytes in such multi-assay plates directly without employing a standard curve.

Abstract translation: 公开了用于确定含有溶解或悬浮在溶剂中的分析物的液体样品的光程长度的光度测定方法和装置。 所述方法和装置依赖于确定溶剂的光吸收性质和含有溶剂的液体样品的光程长度之间的关系。 该关系用于建立含有未知浓度的分析物但具有相似溶剂组成的样品的光程。 进一步公开了用于确定这些样品中分析物浓度的方法和装置，其中光程长和分析物浓度都是未知的。 方法和装置依赖于在不同波长的光下分别确定溶剂的光吸收和被分析物的光吸收。 被分析物的光吸收以及如此确定的光程长度被用于计算分析物的浓度。 用于执行该方法的装置特别有利地包括垂直光束光度计，其包含设置在多测定板的孔内的样品，其中光度计能够监测包括在可见光或可见光区域中的多个波长的每个样品的光吸收 的光谱，以及电磁光谱的近红外区域。 描述了新的光度计装置，其自动确定这种多测定板中分析物的浓度，而不使用标准曲线。

公开(公告)号：WO1990004786A1

公开(公告)日：1990-05-03

申请号：PCT/US1989004320

申请日：1989-10-10

Applicant: MOLECULAR DEVICES CORPORATION ,  OLSON, John, D. ,  ZUK, Robert, F. ,  ARMENTA, Richard, D. ,  BURKE, Charles, R. ,  KUNG, Viola, T. ,  SHELDON, Edward, L., III

Inventor： MOLECULAR DEVICES CORPORATION

IPC: G01N33/53

CPC classification number: G01N33/54353 ,  C07H21/00 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6876 ,  G01N33/537 ,  G01N33/54306 ,  C12Q2563/131 ,  C12Q2522/101

Abstract: The present invention encompasses a capture membrane comprising a porous filter membrane having a hapten bound directly or indirectly to the membrane wherein complexes formed by specific binding having an anti-hapten bound to a binding member of the specifically binding complex are removed from a solution by the hapten as the solution passes through the membrane. In the preferred embodiment biotin is the hapten and avidin or streptavidin is the anti-hapten.

Abstract translation: 本发明包括捕获膜，其包含具有直接或间接结合膜的半抗原的多孔过滤膜，其中通过特异性结合形成的具有与结合特异性结合复合物的结合成员结合的抗半抗原形成的复合物通过 半抗原溶液通过膜。 在优选实施方案中，生物素是半抗原，抗生物素蛋白或链霉亲和素是抗半抗原。

公开(公告)号：WO2003014701A2

公开(公告)日：2003-02-20

申请号：PCT/US2002/025046

申请日：2002-08-07

Applicant: MOLECULAR DEVICES CORPORATION ,  KLAUBERT, Dieter ,  DIWU, Zhenjun ,  YI, Guoliang ,  KIRK, Martin

Inventor： KLAUBERT, Dieter ,  DIWU, Zhenjun ,  YI, Guoliang ,  KIRK, Martin

IPC: G01N

CPC classification number: G01N33/52 ,  Y10S435/975

Abstract: The invention encompasses an improved method for measuring membrane potential using compounds of the formula I as petentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPR TR , fluorescent imaging plate reader, sold by Molecular Device Corp., of Sunnyvale, CA; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cells. X is O. or S. n is 1 or 2.

Abstract translation: 本发明包括使用式I化合物作为测量探针来测量膜电位的改进方法。 这些探针可以与其他荧光指示剂如Indo-1，Fura-2和Fluo-3组合使用，这样的探针可以用于微孔板读取装置，例如FLIPR ，荧光成像读板器，由Molecular 加利福尼亚州桑尼维尔的Device Corp.; 流式细胞仪 和荧光计。 这种探针用于测量活细胞中的膜电位。 X是O.或S.n是1或2。

公开(公告)号：WO1990008313A1

公开(公告)日：1990-07-26

申请号：PCT/US1990000168

申请日：1990-01-17

Applicant: MOLECULAR DEVICES CORPORATION ,  PONTIS, George ,  LACY, Michael ,  DRBAL, Vladimir ,  CHOW, Calvin ,  SMITH, Thomas ,  SMETHERS, Rick ,  ROSS, Debra, A. ,  KIRK, Gregory ,  BRIGGS, Jonathan ,  LEYTES, Lev ,  MEHTA, Suresh, N.

Inventor： MOLECULAR DEVICES CORPORATION

IPC: G01N27/30

CPC classification number: G01N35/00009 ,  G01N33/5302 ,  G01N33/54366 ,  G01N35/00029 ,  G01N2035/00138 ,  G01N2035/00485 ,  Y10S435/97 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/25375

Abstract: This invention encompasses a work station for conducting assays which comprises a filtering assembly (2), a reading assembly (40), and a filter element (4) with a porous filter membrane (5), wherein the filter element has a means (3) for aligning the filter element (4) within the filter assembly (2) to filter reagents on predetermined portions of the porous filter membrane (5) and also to position the filter element in the reading assembly (40) so that detection will occur in those same predetermined locations where analyte or analyte complexes have been filtered.

公开(公告)号：WO1990004645A1

公开(公告)日：1990-05-03

申请号：PCT/US1989004434

申请日：1989-10-06

Applicant: MOLECULAR DEVICES CORPORATION

Inventor： MOLECULAR DEVICES CORPORATION ,  PARCE, John, W. ,  McCONNELL, Harden, M. ,  HUMPHRIES, Gillian, M. ,  KERCSO, Karen, M. ,  OWICKI, John, C. ,  KERCSO, Josef, Elek

IPC: C12Q01/02

CPC classification number: G01N33/5008 ,  C12M23/12 ,  C12M35/08 ,  Y10S435/817

Abstract: Methods and apparatus are disclosed for detecting the effects of cell affecting agents on living cells. The method steps include providing living cells that are retained in a micro flow chamber. The micro flow chamber is adapted for either continuous or intermittent flow of solutions or suspensions in intimate contact with the cells. The solutions or suspensions, which contain a cell affecting agent, are then flowed in intimate contact with the cells and at least one effect of the cell affecting agent on the cells is measured by an appropriate detecting means, which is operatively associated with the micro flow chamber.

Abstract translation: 公开了用于检测细胞影响剂对活细胞的影响的方法和装置。 方法步骤包括提供保留在微流动室中的活细胞。 微流动室适于与细胞紧密接触的连续或间歇流动的溶液或悬浮液。 然后将含有细胞影响剂的溶液或悬浮液与细胞紧密接触流动，并通过合适的检测手段测量细胞影响剂对细胞的至少一种作用，该检测手段与微量流可操作地相关联 室。

公开(公告)号：WO2006039505A3

公开(公告)日：2006-04-13

申请号：PCT/US2005/035215

申请日：2005-09-30

Applicant: MOLECULAR DEVICES CORPORATION

Inventor： PARKER, David ,  ATKINSON, Paul. ,  KIELAR, FILIP ,  BOGE, Annegret ,  SPORTSMAN, Richard, J. ,  GAUDET, Elizabeth ,  YI, George, G.

IPC: C07F5/00

Abstract: Systems, including compositions, kits, and methods, particularly for photoluminescence applications. The systems may include, among others, (1) organic chelators, (2) complexes between the chelators and lanthanide ions, and (3) precursors, derivatives, and uses thereof. The chelators may include a 1,4,7,10-tetraazacyclododecane ring system, for example, having the formula:(I) Here, R 1 , R 2 , and R 3 are substituents of the tetraazacyclododecane ring system, that is further substituted at the 10-position by a sensitizer Z that is typically a polyheterocyclic ring system. The organic chelator may be capable of forming a luminescent complex with a lanthanide ion, and is optionally further substituted by a reactive functional group or a conjugated substance. The resulting lanthanide complex may be useful in luminescence-based assays, such as energy transfer assays, among others.

公开(公告)号：WO2003099780A3

公开(公告)日：2003-12-04

申请号：PCT/US2003/016530

申请日：2003-05-23

Applicant: MOLECULAR DEVICES CORPORATION ,  DIWU, Zhenjun ,  LIAO, Jinfang ,  WANG, Zhiqiang

Inventor： DIWU, Zhenjun ,  LIAO, Jinfang ,  WANG, Zhiqiang

IPC: C12Q1/37

Abstract: Systems, including compositions, kits, and methods, suitable for performing enzyme assays, such as protease assays. The composition may include derivatives of rhodamine 110 that are substitued at the xanthylium nitrogens by a peptide moiety and a morpholine-4-carbonyl moiety, respectively. These compositions may be luminogenic substrates for a large variety of protease enzymes, with utility in a variety of sensitive protease enzyme assays.

公开(公告)号：WO2003099780A2

公开(公告)日：2003-12-04

申请号：PCT/US2003/016530

申请日：2003-05-23

Applicant: MOLECULAR DEVICES CORPORATION ,  DIWU, Zhenjun ,  LIAO, Jinfang ,  WANG, Zhiqiang

Inventor： DIWU, Zhenjun ,  LIAO, Jinfang ,  WANG, Zhiqiang

IPC: C07D

CPC classification number: C07K5/021 ,  C07D311/82 ,  C07D493/10 ,  C07K7/02 ,  C12Q1/37

Abstract: Systems, including compositions, kits, and methods, suitable for performing enzyme assays, such as protease assays. The composition may include derivatives of rhodamine 110 that are substitued at the xanthylium nitrogens by a peptide moiety and a morpholine-4-carbonyl moiety, respectively. These compositions may be luminogenic substrates for a large variety of protease enzymes, with utility in a variety of sensitive protease enzyme assays.

Abstract translation: 适用于进行酶测定的系统，包括组合物，试剂盒和方法，例如蛋白酶测定。 组合物可以包括分别由肽部分和吗啉-4-羰基部分在xanthylium氮取代的罗丹明110的衍生物。 这些组合物可以是用于各种蛋白酶的发光底物，可用于各种灵敏的蛋白酶测定。

公开(公告)号：WO1994003791A1

公开(公告)日：1994-02-17

申请号：PCT/US1993007177

申请日：1993-07-30

Applicant: MOLECULAR DEVICES CORPORATION ,  HAFEMAN, Dean, G. ,  CRAWFORD, Kimberly, L. ,  SANCHEZ, Anthony, J. ,  WADA, Henry, Garrett

Inventor： MOLECULAR DEVICES CORPORATION

IPC: G01N21/31

CPC classification number: C12M41/46 ,  C12M41/26 ,  G01N21/253

Abstract: The invention disclosed is a method for monitoring the metabolism of cells retained in individual wells of a multiassay plate by measuring the rates of extracellular acidification comprising the steps of: placing the cells in a solution containing a pH buffer and an acid/base indicator (the acidic form having optimum absorbance in a first wavelength region and the basic form having optimum absorbance in a second wavelength region), heating the solution in the individual wells to a substantially uniform temperature, mixing the solution in each well, measuring the optical densities of light passing vertically through the wells at a first wavelength in the first wavelength region and a second wavelength in the second wavelength region, determining a ratio of the optical density at the first and second wavelengths in each of two, or more wells, and repeating the mixing, measuring, and determining steps to monitor kinetically a rate of change in extracellular pH caused by the cells. The use of selected buffering system-acid/base indicator pairs, which provide little or no optically-apparent change in pH with change in temperature, was found to be highly desirable for carrying out the method. Methods for composing such buffering system-acid/base indicator pairs are disclosed, together with examples of such pairs.

Abstract translation: 本发明公开了一种通过测量细胞外酸化速率来监测多分析板单个孔中保留细胞代谢的方法，包括以下步骤：将细胞置于含有pH缓冲液和酸/碱指示剂的溶液中 在第一波长区域具有最佳吸光度的酸性形式和在第二波长区域中具有最佳吸光度的碱性形式），将各孔中的溶液加热至基本均匀的温度，将溶液混合在每个孔中，测量光的光密度 在第一波长区域中以第一波长垂直穿过阱并且在第二波长区域中穿过第二波长，确定两个或更多个孔中的每一个中的第一和第二波长处的光密度的比率，并重复混合 ，测量和确定动态监测由细胞引起的细胞外pH变化速率的步骤。 发现使用选择的缓冲系统 - 酸/碱指示器对，其提供很少或没有光学上明显的pH随温度变化的变化，对于进行该方法是非常需要的。 公开了组合这种缓冲系统酸/碱指示剂对的方法以及这些对的实例。