公开(公告)号：US12265011B2

公开(公告)日：2025-04-01

申请号：US18154195

申请日：2023-01-13

Applicant: Postnova Analytics GmbH

Inventor： Horst Reichert

IPC: G01N15/1031 ,  G01N15/10 ,  G01R15/22

Abstract: A method for determining a physical property related to a charge of a constituent of a sample, from field flow fractionation measurements with an additional electrical field, comprising the steps of obtaining a first fractogram of a first sample and a second fractogram of a second sample, wherein the first sample and the second sample are samples of a same substance, the first fractogram has been generated using a first electrical field, the second fractogram has been generated using a second electrical field, and a strength of the first electrical field and a strength of the second electrical field are different from each other; determining, by using a first mapping, from a first intensity value of the first fractogram, a first value and determining, by using a second mapping, from a second intensity value of the second fractogram, a second value; and determining, based on the first value and the second value, a physical property related to a charge of a constituent of at least one of the first sample and the second sample; wherein the first mapping maps the first intensity value to the first value of a first bijective function over time and the second mapping maps the second intensity value to the second value of a second bijective function over time.

公开(公告)号：US20240241027A1

公开(公告)日：2024-07-18

申请号：US18154195

申请日：2023-01-13

Applicant: Postnova Analytics GmbH

Inventor： Horst Reichert

IPC: G01N15/10

CPC classification number: G01N15/1031 ,  G01N2015/1062

Abstract: A method for determining a physical property related to a charge of a constituent of a sample, from field flow fractionation measurements with an additional electrical field, comprising the steps of obtaining a first fractogram of a first sample and a second fractogram of a second sample, wherein the first sample and the second sample are samples of a same substance, the first fractogram has been generated using a first electrical field, the second fractogram has been generated using a second electrical field, and a strength of the first electrical field and a strength of the second electrical field are different from each other; determining, by using a first mapping, from a first intensity value of the first fractogram, a first value and determining, by using a second mapping, from a second intensity value of the second fractogram, a second value; and determining, based on the first value and the second value, a physical property related to a charge of a constituent of at least one of the first sample and the second sample; wherein the first mapping maps the first intensity value to the first value of a first bijective function over time and the second mapping maps the second intensity value to the second value of a second bijective function over time.

公开(公告)号：US08760652B2

公开(公告)日：2014-06-24

申请号：US13654747

申请日：2012-10-18

Applicant: Postnova Analytics GmbH

Inventor： Dietheim Fröse ,  Roland Welz

IPC: G01N21/00

CPC classification number: G01N21/51 ,  G01N21/53 ,  G01N30/74 ,  G01N2021/4711 ,  G01N2201/064

Abstract: An apparatus for measuring the light scattering properties of a sample in a liquid medium, wherein the liquid medium with the sample is illuminated by a laser beam in a measuring cell transversely to the direction of filling the liquid medium in the measuring cell or transversely to the flow direction of the liquid medium within the measuring cell, comprising a laser, a cylindrical measuring cell, a first inner aperture system, a second outer aperture system and at least two detectors, wherein the detectors are arranged outside of the second outer aperture system so that they collect the light scattered on the sample within set, different angle ranges, wherein the first inner aperture system and the second outer aperture system are formed and arranged circularly and concentrically around the axis of the measuring cell. Use of the apparatus and a method that makes use of the apparatus are also disclosed.

Abstract translation: 一种用于测量液体介质中的样品的光散射性质的装置，其中具有样品的液体介质通过横向于在测量池中填充液体介质的方向的横向于横向于测量单元的测量单元中的激光束照射 测量单元内的液体介质的流动方向，包括激光器，圆柱形测量单元，第一内孔系统，第二外孔系统和至少两个检测器，其中检测器布置在第二外孔系统的外侧，因此 它们收集在集合的不同角度范围内散射在样品上的光，其中第一内孔系统和第二外孔系统被形成并围绕测量池的轴线圆周且同心地布置。 还公开了使用该装置和使用该装置的方法。

公开(公告)号：WO2012160019A1

公开(公告)日：2012-11-29

申请号：PCT/EP2012/059352

申请日：2012-05-21

Applicant: POSTNOVA ANALYTICS GMBH ,  WELZ, Roland

Inventor： WELZ, Roland

IPC: G01N30/00

CPC classification number: G01N30/0005 ,  G01N2030/003

Abstract: Die vorliegende Erfindung betrifft ein Verfahren zur Probentrennung mittels Hohlfaser Fluss Feld-Fluss Fraktionierung (HF5) sowie eine Vorrichtung zur Durchführung dieses Verfahrens. Das erfindungsgemäße Verfahren umfasst (i) die Injektion einer Probe unter Verwendung eines Laufmittels in eine oder mehrere Hohlfaser (n), (ii) die Fokussierung der Probe mit Hilfe des Laufmittels in der/den Hohlfaser (n) und ( iii) die Elution der Probe mit dem Laufmittel aus der/den Hohlfaser (n) unter dem Einfluss eines Trennfeldes und die Detektion der aufgetrennten Probe mit einem oder mehreren Detektor(en) und ist dadurch gekennzeichnet, dass der Volumenstrom und die Flussrichtung des Laufmittels durch den/die Detektor(en) zumindest während der Schritte (ii) und (iii) einschließlich des Übergangs von Schritt (ii) zu Schritt (iii) gleich bleiben.

Abstract translation: 本发明涉及一种通过中空纤维流场流分级（HF5）的手段样品分离，以及用于执行此方法的装置的方法。 本发明的方法包括：（i）样品的在一个或多个中空纤维（一个或多个）使用的流动相的注入，（ii）所述样品的聚焦在/中空纤维（多个）洗脱液的助剂和（iii）的洗脱 具有释放场的影响下的/中空纤维（或多个）的溶剂和检测分离的样品的一个或多个检测器（一个或多个）和样品的特征在于：体积流量和洗脱液的流动方向通过/检测器（ 或多个）至少在步骤（ii）和（iii）包括步骤的过渡（ii）向步骤（iii）保持相同。

公开(公告)号：US10132782B2

公开(公告)日：2018-11-20

申请号：US15042398

申请日：2016-02-12

Applicant: Postnova Analytics GmbH

Inventor： Roland Welz ,  Thorsten Klein

IPC: G01N30/00 ,  B01D11/04 ,  G01N30/74

Abstract: The present invention relates to an apparatus for field-flow fractionation and to a method for separating samples by means of field-flow fractionation using this apparatus. The apparatuses of the invention comprise a separation channel which, in addition to a first outlet for sample-containing solvent, comprises a second outlet for sample-free solvent, wherein the second outlet is arranged in a region of the separation channel from which sample-free solvent may be removed during elution and a flow volume control device is arranged downstream of the second outlet. They are characterized in that the flow volume control device is a mass flow controller.

公开(公告)号：US20130100433A1

公开(公告)日：2013-04-25

申请号：US13654747

申请日：2012-10-18

Applicant: Postnova Analytics GmbH

Inventor： Diethelm Frose ,  Roland Welz

IPC: G01N21/51

CPC classification number: G01N21/51 ,  G01N21/53 ,  G01N30/74 ,  G01N2021/4711 ,  G01N2201/064

Abstract: An apparatus for measuring the light scattering properties of a sample in a liquid medium, wherein the liquid medium with the sample is illuminated by a laser beam in a measuring cell transversely to the direction of filling the liquid medium in the measuring cell or transversely to the flow direction of the liquid medium within the measuring cell, comprising a laser, a cylindrical measuring cell, a first inner aperture system, a second outer aperture system and at least two detectors, wherein the detectors are arranged outside of the second outer aperture system so that they collect the light scattered on the sample within set, different angle ranges, wherein the first inner aperture system and the second outer aperture system are formed and arranged circularly and concentrically around the axis of the measuring cell. Use of the apparatus and a method that makes use of the apparatus are also disclosed.

Abstract translation: 一种用于测量液体介质中的样品的光散射性质的装置，其中具有样品的液体介质通过横向于在测量池中填充液体介质的方向的横向于横向于测量单元的测量单元中的激光束照射 测量单元内的液体介质的流动方向，包括激光器，圆柱形测量单元，第一内孔系统，第二外孔系统和至少两个检测器，其中检测器布置在第二外孔系统的外侧，因此 它们收集在集合的不同角度范围内散射在样品上的光，其中第一内孔系统和第二外孔系统被形成并围绕测量池的轴线圆周且同心地布置。 还公开了使用该装置和使用该装置的方法。

公开(公告)号：WO2012160018A1

公开(公告)日：2012-11-29

申请号：PCT/EP2012/059350

申请日：2012-05-21

Applicant: POSTNOVA ANALYTICS GMBH ,  WELZ, Roland

Inventor： WELZ, Roland

IPC: B04B5/04 ,  B04B9/12 ,  B04B11/02 ,  G01N30/00

CPC classification number: B04B15/06 ,  B04B5/0442 ,  B04B9/12 ,  B04B11/02 ,  G01N30/0005 ,  G01N2030/002

Abstract: The current invention relates to an apparatus for performing centrifugal field- flow fractionation. In particular, in the apparatus according to the invention, radial rotary shaft seals (36 a/b) are used for sealing the rotating parts of the shaft (61) for carrying out the centrifugal field- flow fractionation and the stationary parts. Furthermore, a separate line is introduced for reducing the pressure differential across these seals. In such way, the driving force driving sample fluid across the seals is reduced, thereby reducing leakage.

Abstract translation: 本发明涉及一种用于进行离心场流分馏的装置。 特别地，在根据本发明的装置中，径向旋转轴密封件（36a / b）用于密封轴（61）的旋转部分，用于进行离心流 - 流分馏和静止部分。 此外，引入了一条单独的管线，以减少跨越这些密封件的压力差。 以这种方式，通过密封件驱动样品流体的驱动力减小，从而减少泄漏。

公开(公告)号：US09897577B2

公开(公告)日：2018-02-20

申请号：US14796055

申请日：2015-07-10

Applicant: Postnova Analytics GmbH

Inventor： Thorsten Klein

IPC: G01N1/00 ,  G01N30/32 ,  G01N30/00 ,  G01N30/38

CPC classification number: G01N30/32 ,  G01N30/0005 ,  G01N30/38 ,  G01N2030/324 ,  G01N2030/326 ,  G01N2030/328

Abstract: The present invention relates to an apparatus for field-flow fractionation and to a method for separating samples by means of field-flow fractionation using this apparatus. The apparatuses of the invention comprise one or more reservoirs, a pump, a first flow volume splitting device, two valves for flow control, an injector, a separation channel having a first connector at a first end and a second connector at a second end or an AF4 separation channel having a first connector at a first end, a second connector at a second end and a third connector between the first and the second ends, optionally a second flow volume splitting device, a back-pressure element, one or more detector(s), a flow volume control device and one or more waste container(s). The apparatuses of the invention are characterized in that they comprise a valve for flow control in a first flow path and a second flow path which connect the pump to the first or the second or the third connector of the separation channel and that these valves are switched alternately in a controllable time ratio.

公开(公告)号：US09329082B2

公开(公告)日：2016-05-03

申请号：US14294450

申请日：2014-06-03

Applicant: Postnova Analytics GmbH

Inventor： Roland Welz

IPC: G01J1/42 ,  G01J1/08 ,  G01N15/02 ,  G01N21/51 ,  G01N21/47

CPC classification number: G01J1/42 ,  G01J1/08 ,  G01J2001/4295 ,  G01N15/0205 ,  G01N21/51 ,  G01N2021/4707 ,  G01N2021/4711

Abstract: The invention provides a method for measuring light scattered on a sample in a medium, in particular a fluid medium, that comprises the following steps: providing a rotatably arranged measuring cell with a substantially circular cross-section in a plane perpendicular to the axis of rotation for receiving the medium and the sample, rotating the measuring cell, preferably at least once by substantially 360°, about the axis of rotation, in particular by means of a drive, emitting a laser beam by means of a laser onto the sample located within the measuring cell in the plane perpendicular to the axis of rotation at different angles of rotation of the measuring cell, the measuring cell maintaining its position in the direction of the axis of rotation, detecting scattered light signals by means of at least two detectors arranged in a circle and concentrically to the center of rotation of the measuring cell and fixed within set, different angular ranges at different angles of rotation of the measuring cell, and determining a corrected signal value for each detector on the basis of the scattered light signals detected at different angles of rotation of the measuring cell for each detector. Furthermore, the invention provides an apparatus for measuring light scattered on a sample according to the method comprising a laser, a measuring cell and a detector.

Abstract translation: 本发明提供一种用于测量在介质，特别是流体介质中的样品上散射的光的方法，该方法包括以下步骤：在垂直于旋转轴线的平面中提供具有基本圆形横截面的可旋转布置的测量单元 用于接收介质和样品，特别是借助于驱动器，将测量单元，优选至少一次旋转约360°旋转轴线，通过激光将激光束发射到位于 所述测量单元在垂直于所述测量单元的不同旋转角度的旋转轴线的平面中，所述测量单元保持其在所述旋转轴线的方向上的位置，通过布置在所述测量单元中的至少两个检测器来检测散射光信号 圆圈并同心对准测量单元的旋转中心并固定在不同的角度范围内，在不同的旋转角度 基于在每个检测器的测量单元的不同旋转角度检测的散射光信号，确定每个检测器的校正信号值。 此外，本发明提供了一种根据包括激光，测量单元和检测器的方法测量在样品上散射的光的装置。

公开(公告)号：US20140354990A1

公开(公告)日：2014-12-04

申请号：US14294450

申请日：2014-06-03

Applicant: Postnova Analytics GmbH

Inventor： Roland Welz

IPC: G01J1/42 ,  G01J1/08 ,  G01N15/02

CPC classification number: G01J1/42 ,  G01J1/08 ,  G01J2001/4295 ,  G01N15/0205 ,  G01N21/51 ,  G01N2021/4707 ,  G01N2021/4711

Abstract: The invention provides a method for measuring light scattered on a sample in a medium, in particular a fluid medium, that comprises the following steps: providing a rotatably arranged measuring cell with a substantially circular cross-section in a plane perpendicular to the axis of rotation for receiving the medium and the sample, rotating the measuring cell, preferably at least once by substantially 360°, about the axis of rotation, in particular by means of a drive, emitting a laser beam by means of a laser onto the sample located within the measuring cell in the plane perpendicular to the axis of rotation at different angles of rotation of the measuring cell, the measuring cell maintaining its position in the direction of the axis of rotation, detecting scattered light signals by means of at least two detectors arranged in a circle and concentrically to the centre of rotation of the measuring cell and fixed within set, different angular ranges at different angles of rotation of the measuring cell, and determining a corrected signal value for each detector on the basis of the scattered light signals detected at different angles of rotation of the measuring cell for each detector. Furthermore, the invention provides an apparatus for measuring light scattered on a sample according to the method comprising a laser, a measuring cell and a detector.

Abstract translation: 本发明提供一种用于测量在介质，特别是流体介质中的样品上散射的光的方法，该方法包括以下步骤：在垂直于旋转轴线的平面中提供具有基本圆形横截面的可旋转布置的测量单元 用于接收介质和样品，特别是借助于驱动器，将测量单元，优选至少一次旋转约360°旋转轴线，通过激光将激光束发射到位于 所述测量单元在垂直于所述测量单元的不同旋转角度的旋转轴线的平面中，所述测量单元保持其在所述旋转轴线的方向上的位置，通过布置在所述测量单元中的至少两个检测器来检测散射光信号 圆圈并同心对准测量单元的旋转中心并固定在不同的角度范围内，在不同的旋转角度 基于在每个检测器的测量单元的不同旋转角度检测的散射光信号，确定每个检测器的校正信号值。 此外，本发明提供了一种根据包括激光，测量单元和检测器的方法测量在样品上散射的光的装置。