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    METHOD AND APPARATUS FOR GEL ELECTROPHORETIC IMMUNOASSAY
    5.
    发明申请
    METHOD AND APPARATUS FOR GEL ELECTROPHORETIC IMMUNOASSAY 审中-公开
    用于凝胶电泳免疫测定的方法和设备

    公开(公告)号：WO2007032788A2

    公开(公告)日：2007-03-22

    申请号：PCT/US2006/019463

    申请日：2006-05-18

    Applicant: SANDIA NATIONAL LABORATORIES

    Inventor： HERR, Amy, Elizabeth ,  SINGH, Anup, K. ,  THROCKMORTON, Daniel, J.

    IPC: B01D57/02 ,  G01N27/447

    CPC classification number: G01N27/44726 ,  B82Y10/00 ,  B82Y30/00 ,  G01N27/44747

    Abstract: A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel electrophoretic separation and quantifying analyte concentration based upon conventional polyacrylamide gel electrophoresis (PAGE). To retain biological activity of proteins and maintain intact immune complexes, native PAGE conditions were employed. Both direct (non-competitive) and competitive immunoassay formats are demonstrated in microchips for detecting toxins and biomarkers (cytokines, c-reactive protein) in bodily fluids (serum, saliva, oral fluids). Further, a description of gradient gels fabrication is included, in an effort to describe methods we have developed for further optimization of on-chip PAGE immunoassays. The described chip-based PAGE immunoassay method enables immunoassays are fast (minutes) and require very small amounts of sample (less than a few microliters). Use of microfabricated chips as a platform enables integration, parallel assays, automation and development of portable devices.

    Abstract translation: 通过将光聚合的交联聚丙烯酰胺凝胶集成在微流体装置内，开发了用于执行基于电泳的免疫测定的微分析平台。 基于常规聚丙烯酰胺凝胶电泳（PAGE），通过凝胶电泳分离和定量分析物浓度进行微流体免疫测定。 为了保留蛋白质的生物学活性并维持完整的免疫复合物，采用天然PAGE条件。 用于检测体液（血清，唾液，口腔液）中的毒素和生物标志物（细胞因子，c-反应蛋白）的微芯片中都证明了直接（非竞争性）和竞争性免疫测定形式。 此外，还包括对梯度凝胶制造的描述，以描述我们为进一步优化片上PAGE免疫测定而开发的方法。 所描述的基于芯片的PAGE免疫测定方法使免疫测定快速（分钟）并且需要非常少量的样品（少于几微升）。 使用微型芯片作为平台可实现便携式设备的集成，并行分析，自动化和开发。

    FAST RESPONSE NANOFIBER ARTICLES WITH TUNABLE WETTABILITY AND BULK PROPERTIES
    6.
    发明申请
    FAST RESPONSE NANOFIBER ARTICLES WITH TUNABLE WETTABILITY AND BULK PROPERTIES 审中-公开
    具有易挥发性和膨胀性的快速响应纳米纤维制品

    公开(公告)号：WO2013032859A1

    公开(公告)日：2013-03-07

    申请号：PCT/US2012/052070

    申请日：2012-08-23

    Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. ,  SANDIA NATIONAL LABORATORIES, NEW MEXICO ,  SIGMUND, Wolfgang, M. ,  MUTHIAH, Palanikkumaran ,  BOYLE, Tim

    Inventor： SIGMUND, Wolfgang, M. ,  MUTHIAH, Palanikkumaran ,  BOYLE, Tim

    IPC: D04H1/544 ,  D06M13/282 ,  D01F6/04 ,  D06M15/333

    CPC classification number: D04H1/728 ,  D01D5/0007 ,  D01F6/36 ,  D01F6/56 ,  D04H1/544 ,  Y10T442/615 ,  Y10T442/626

    Abstract: A fibrous properties-switching article comprises a mat consisting of fibers having a fiber diameter of 2 microns or less. The fibers comprise a polymer, copolymer, polymer blend, or polymer network, wherein the fibers have a diameter of 2 μιη or less. The surface and/or bulk property of the mat changes over a range of temperatures, wherein the polymer, copolymer, polymer blend, or polymer network undergoes a structural change over the range of temperatures. The fiber mat is formed by electrospinning. In an exemplary embodiment, a blend of polystyrene and poly((N-isopropyl acrylamide) (bl-PS/PNIPA) in dimethylformamide (DMF) is electrospun to form a mat consisting of fibers with a diameter less than 2 μηι that shows a transition from a superhydrophilic surface to a nearly superhydrophobic surface over a temperature range from 30 °C to 45 °C. A fiber mat formed by electrospinning a DMF solution comprising poly(N-isopropyl acrylamide-co- methacylicacid) (PNIPAMAA), comprises fibers having a diameter less than 2 μιτι and are cross linked after spinning. The crosslinked PNIPAMAA, (xl-PNIPAMAA) fiber mat displays a transition from a hydrophilic surface to a nearly hydrophobic surface over a temperature range from 30 °C to 45 °C.

    Abstract translation: 纤维性质切换制品包括由纤维直径为2微米或更小的纤维组成的垫。 纤维包括聚合物，共聚物，聚合物共混物或聚合物网络，其中纤维的直径为2μΩ 或更少。 垫的表面和/或体积特性在一定温度范围内变化，其中聚合物，共聚物，聚合物共混物或聚合物网络在温度范围内经历结构变化。 纤维垫通过静电纺丝形成。 在一个示例性实施方案中，将聚苯乙烯和聚（（N-异丙基丙烯酰胺）（b1-PS / PNIPA））在二甲基甲酰胺（DMF）中的共混物静电纺丝以形成由直径小于2μΩ的纤维组成的垫， 通过静电纺丝包含聚（N-异丙基丙烯酰胺 - 共 - 甲酰甲酸）（PNIPAMAA）的DMF溶液形成的纤维垫，形成由纤维垫组成的纤维垫，其包含： 直径小于2μt·t的纤维，在纺丝后交联，交联的PNIPAMAA（xl-PNIPAMAA）纤维垫在30℃至30℃的温度范围内显示从亲水表面到接近疏水性表面的转变 45°C。

    WAVE-PLATE STRUCTURES, POWER SELECTIVE OPTICAL FILTER DEVICES, AND OPTICAL SYSTEMS USING SAME
    7.
    发明申请
    WAVE-PLATE STRUCTURES, POWER SELECTIVE OPTICAL FILTER DEVICES, AND OPTICAL SYSTEMS USING SAME 审中-公开
    波片结构，电源选择性光学滤波器，以及使用该光学系统的光学系统

    公开(公告)号：WO2011139470A2

    公开(公告)日：2011-11-10

    申请号：PCT/US2011/031446

    申请日：2011-04-06

    Applicant: SANDIA NATIONAL LABORATORIES ,  KOPLOW, Jeffrey, P.

    Inventor： KOPLOW, Jeffrey, P.

    IPC: G02B26/02

    CPC classification number: G02B27/288

    Abstract: In an embodiment, an optical filter device includes an input polarizer for selectively transmitting an input signal. The device includes a wave-plate structure positioned to receive the input signal, which includes first and second substantially zero-order, zero-wave plates arranged in series with and oriented at an angle relative to each other. The first and second zero-wave plates are configured to alter a polarization state of the input signal passing in a manner that depends on the power of the input signal. Each zero-wave plate includes an entry and exit wave plate each having a fast axis, with the fast axes oriented substantially perpendicular to each other. Each entry wave plate is oriented relative to a transmission axis of the input polarizer at a respective angle. An output polarizer is positioned to receive a signal output from the wave -plate structure and selectively transmits the signal based on the polarization state.

    Abstract translation: 在一个实施例中，滤光器装置包括用于选择性地传输输入信号的输入偏振器。 该装置包括波片结构，其定位成接收输入信号，该输入信号包括与彼此串联并且相对于彼此成一定角度排列的第一和第二基本上零级的零波片。 第一和第二零波片被配置为以取决于输入信号的功率的方式改变通过的输入信号的偏振状态。 每个零波片包括各自具有快轴的进出波片，其中快轴基本上彼此垂直。 每个进入波片相对于输入偏振器的透射轴以相应的角度定向。 输出偏振器被定位成接收从波片结构输出的信号，并且基于偏振状态选择性地发送信号。

    FABRIC WITH BALANCED MODULUS AND UNBALANCED CONSTRUCTION
    10.
    发明申请
    FABRIC WITH BALANCED MODULUS AND UNBALANCED CONSTRUCTION 审中-公开
    具有平衡模块和不平衡结构的织物

    公开(公告)号：WO1998017505A1

    公开(公告)日：1998-04-30

    申请号：PCT/US1997018567

    申请日：1997-10-20

    Applicant: PRECISION FABRICS GROUP, INC. ,  SANDIA NATIONAL LABORATORIES ,  GWINN, Kenneth, W. ,  HOLDER, Joseph, P.

    Inventor： PRECISION FABRICS GROUP, INC. ,  SANDIA NATIONAL LABORATORIES

    IPC: B60R21/16

    CPC classification number: B60R21/235 ,  D03D1/02

    Abstract: This disclosure describes fabric and a method for making fabric, for use in a flexible pressure vessel or other fabric structures, which has an unbalanced woven construction and has a balanced or nearly balanced modulus of elasticity for the end-use conditions of this fabric. Unbalanced woven fabrics that do not have balanced properties produce unequal stretching and are susceptible to premature failure. The present invention of unbalanced woven fabric construction with balanced mechanical properties such as modulus of elasticity can be used to optimize fabric strength, mass, volume, and performance characteristics and also reduce manufacturing costs for various fabric structure applications. Balanced mechanical properties for the fabric structure are achieved by altering the force of the fabric by increasing or decreasing the number of yarns in a particular direction of the fabric, by changing the strain during stretch of the fabric, or by alternative weaves of the fabric.

    Abstract translation: 本公开描述了用于制造织物的织物和方法，用于柔性压力容器或其它织物结构，其具有不平衡的编织结构，并且对于织物的最终使用条件具有平衡或接近平衡的弹性模量。 不平衡的不平衡织物产生不均匀的拉伸并且容易发生过早的失效。 可以使用具有平衡机械性能如弹性模量的不平衡织物结构的本发明来优化织物强度，质量，体积和性能特征，并且还降低各种织物结构应用的制造成本。 通过在织物的特定方向上增加或减少纱线数量，通过改变织物伸展期间的应变，或通过织物的替代编织来改变织物的力，来实现织物结构的平衡机械性能。

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