公开(公告)号：AU2007223913A1

公开(公告)日：2007-09-13

申请号：AU2007223913

申请日：2007-03-07

Applicant: PAULA HICKS ,  LUGINBUHL PETER ,  SARA MCFARLAN ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN ,  CARGILL INC ,  BURKE ELLEN ,  STEVEN GORT ,  RICHARDSON TOBY

Inventor： BURKE ELLEN ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  MCFARLAN SARA C ,  GORT STEVEN JOHN ,  RICHARDSON TOBY ,  HICKS PAULA M ,  LUGINBUHL PETER ,  ZHAO LISHAN

IPC: C12P17/00 ,  A23L27/00 ,  A23L27/30 ,  C07D209/20 ,  C12N9/88

公开(公告)号：AU2006341528A1

公开(公告)日：2007-12-06

申请号：AU2006341528

申请日：2006-04-26

Applicant: CARGILL INC ,  BRIAN BRAZEAU ,  BURKE ELLEN ,  SOUZA MERVYN DE ,  STEVEN GORT ,  PAULA HICKS ,  SHERRY KOLLMANN ,  LUGINBUHL PETER ,  SARA MCFARLAN ,  RICHARDSON TOBY ,  FERNANDO SANCHEZ RIERA ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN

Inventor： HICKS PAULA M ,  BRAZEAU BRIAN J ,  ZHAO LISHAN ,  GORT STEVEN J ,  RICHARDSON TOBY ,  SANCHEZ-RIERA FERNANDO A ,  SOUZA MERVYN DE ,  CIMBALA MICHELE A ,  LUGINBUHL PETER ,  KOLLMANN SHERRY R ,  SOLHEID CHRISTOPHER ,  MCFARLAN SARA C ,  BURKE ELLEN ,  WEINER DAVID

IPC: C12N9/88

公开(公告)号：WO2011082363A1

公开(公告)日：2011-07-07

申请号：PCT/US2010/062600

申请日：2010-12-30

Applicant: CARGILL, INCORPORATED ,  HILBERT, Brent Howard ,  PEMBLE, Trent H. ,  PORTER, Michael A. ,  SOLHEID, Christopher

Inventor： HILBERT, Brent Howard ,  PEMBLE, Trent H. ,  PORTER, Michael A. ,  SOLHEID, Christopher

IPC: B01D61/14

CPC classification number: B01D69/02 ,  B01D61/025 ,  B01D61/027 ,  B01D2315/16 ,  B01D2325/14 ,  B01D2325/20 ,  C12P17/10 ,  G01N2030/527

Abstract: Methods and systems for separating and purifying monatin are described. In the production of monatin, a mixture is formed which includes monatin, starting materials and intermediates. The methods and systems include a membrane having a zeta potential of from about -19 to -6 such that the membrane rejects greater than about 90 % of the monatin. In some embodiments, the membrane is a nanofiltration membrane.

Abstract translation: 描述了分离和纯化莫纳甜的方法和系统。 在莫纳甜的生产中，形成了包括莫纳甜，原料和中间体的混合物。 所述方法和系统包括具有约-19至-6的ζ电位的膜，使得膜排斥大于约90％的莫纳甜。 在一些实施方案中，膜是纳滤膜。

公开(公告)号：WO2009088482A1

公开(公告)日：2009-07-16

申请号：PCT/US2008/014137

申请日：2008-12-31

Applicant: CARGILL, INCORPORATED ,  DE SOUZA, Mervyn L. ,  HICKS, Paula M. ,  KOLLMANN, Sherry R. ,  LAPLAZA, José M. ,  LUNDORFF, Joshua M. ,  MCFARLAN, Sara C. ,  MARASCO, Erin ,  NIU, Wei ,  SANCHEZ-RIERA, Fernando A. ,  SOLHEID, Christopher ,  WEINER, David P. ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin

Inventor： DE SOUZA, Mervyn L. ,  HICKS, Paula M. ,  KOLLMANN, Sherry R. ,  LAPLAZA, José M. ,  LUNDORFF, Joshua M. ,  MCFARLAN, Sara C. ,  MARASCO, Erin ,  NIU, Wei ,  SANCHEZ-RIERA, Fernando A. ,  SOLHEID, Christopher ,  WEINER, David P. ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin

IPC: C12P21/06 ,  C12N9/10

CPC classification number: C12P13/227 ,  C12N9/0004 ,  C12N9/1096 ,  C12P17/10

Abstract: The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.

Abstract translation: 本发明提供氨基转移酶和氧化还原酶多肽以及编码这种多肽的核酸。 还提供了使用这种氨基转移酶和氧化还原酶核酸和多肽的方法。

公开(公告)号：WO2007140195A3

公开(公告)日：2007-12-06

申请号：PCT/US2007/069513

申请日：2007-05-23

Applicant: CARGILL, INCORPORATED ,  HILBERT, Brent, H. ,  SOLHEID, Christopher ,  MCFARLAN, Sara, C. ,  KHARE, Anil, B. ,  DESOUZA, Mervyn ,  SANCHEZ-RIERA, Fernando, A. ,  FLOY, Kent ,  HICKS, Paula, M.

Inventor： HILBERT, Brent, H. ,  SOLHEID, Christopher ,  MCFARLAN, Sara, C. ,  KHARE, Anil, B. ,  DESOUZA, Mervyn ,  SANCHEZ-RIERA, Fernando, A. ,  FLOY, Kent ,  HICKS, Paula, M.

IPC: C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

Abstract: Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

公开(公告)号：WO2007103389A2

公开(公告)日：2007-09-13

申请号：PCT/US2007/005718

申请日：2007-03-07

Applicant: CARGILL, INCORPORATED ,  BURKE, Ellen ,  GORT, Steven, John ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  MCFARLAN, Sara, C. ,  RICHARDSON, Toby ,  SOLHEID, Christopher ,  WEINER, David ,  ZHAO, Lishan

Inventor： BURKE, Ellen ,  GORT, Steven, John ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  MCFARLAN, Sara, C. ,  RICHARDSON, Toby ,  SOLHEID, Christopher ,  WEINER, David ,  ZHAO, Lishan

IPC: C12P17/00 ,  C07D209/20 ,  C12N9/88 ,  A23L1/22

CPC classification number: C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R3R and S5R configurations, and salts thereof.

Abstract translation: 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性（包括热稳定性和耐热活性），以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种制药，农业和工业环境中。 在一些实施方案中，本发明提供了用于生产R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体诸如R的多肽和生物合成途径 ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，如R3R和S5R构型及其盐。

公开(公告)号：WO2011082365A3

公开(公告)日：2011-09-09

申请号：PCT/US2010062603

申请日：2010-12-30

Applicant: CARGILL INC ,  MARASCO ERIN ,  PEMBLE TRENT ,  PORTER MICHAEL A ,  RASMUSSEN MARIBETH ,  SOLHEID CHRISTOPHER

Inventor： MARASCO ERIN ,  PEMBLE TRENT ,  PORTER MICHAEL A ,  RASMUSSEN MARIBETH ,  SOLHEID CHRISTOPHER

IPC: C12P17/10 ,  C07D209/18

CPC classification number: C12P7/10 ,  Y02E50/16

Abstract: Methods and systems for increasing the production of monatin in a multi-step equilibrium pathway are described. Tryptophan and pyruvate are added to a bioreactor to form a mixture comprising monatin and a plurality of intermediates via a multi-step equilibrium pathway. The methods and systems include operating the bioreactor such that a temperature of the mixture in the bioreactor is less than 25 degrees Celsius, resulting in an increased production of monatin. In some embodiments, the temperature of the mixture in the bioreactor is between about 5 degrees Celsius and about 23 degrees Celsius; in other embodiments, the temperature is between about 10 degrees Celsius and about 18 degrees Celsius.

Abstract translation: 描述了用于增加莫纳甜在多步平衡途径中产生的方法和系统。 将色氨酸和丙酮酸加入到生物反应器中以通过多步平衡途径形成包含莫纳甜和多种中间体的混合物。 该方法和系统包括操作生物反应器，使得生物反应器中的混合物的温度低于25摄氏度，导致monatin的产生增加。 在一些实施方案中，生物反应器中混合物的温度在约5摄氏度和约23摄氏度之间; 在其他实施例中，温度在大约10摄氏度和大约18摄氏度之间。

公开(公告)号：WO2008085575A2

公开(公告)日：2008-07-17

申请号：PCT/US2007/081932

申请日：2007-10-19

Applicant: CARGILL, INCORPORATED ,  BRAZEAU, Brian, J. ,  DE SOUZA, Mervyn, L. ,  GORT, Steven, J. ,  HICKS, Paula, M. ,  KOLLMANN, Sherry, R. ,  LAPLAZA, Jose, M. ,  MCFARLAN, Sara, C. ,  SANCHEZ-RIERA, Fernando, A. ,  SOLHEID, Christopher

Inventor： BRAZEAU, Brian, J. ,  DE SOUZA, Mervyn, L. ,  GORT, Steven, J. ,  HICKS, Paula, M. ,  KOLLMANN, Sherry, R. ,  LAPLAZA, Jose, M. ,  MCFARLAN, Sara, C. ,  SANCHEZ-RIERA, Fernando, A. ,  SOLHEID, Christopher

IPC: C07H21/04 ,  C12N1/20 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/90 ,  C12P13/02 ,  C12P13/22

CPC classification number: C12N9/1096 ,  C12N9/90 ,  C12P17/10

Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Abstract translation: 使用多肽和生物合成途径产生莫纳甜和莫纳甜的某些立体异构体，例如R，R莫纳甜和S，R莫纳甜及其盐。 这些多肽和生物合成途径也可用于生产在特定莫纳甜合成途径中形成的包括一些生物合成途径的中间体R-2-羟基-2-（吲哚-3-基甲基）-4-酮戊二酸。

公开(公告)号：WO2012166842A1

公开(公告)日：2012-12-06

申请号：PCT/US2012/040078

申请日：2012-05-31

Applicant: CARGILL, INCORPORATED ,  PEMBLE, Trent H. ,  SOLHEID, Christopher ,  HILBERT, Brent H. ,  DICKEY, AnaLisa ,  JOHNSON, Dustin P. ,  PORTER, Michael A.

Inventor： PEMBLE, Trent H. ,  SOLHEID, Christopher ,  HILBERT, Brent H. ,  DICKEY, AnaLisa ,  JOHNSON, Dustin P. ,  PORTER, Michael A.

IPC: C12P17/10 ,  C12P13/00

CPC classification number: C12P17/10 ,  B01D61/025 ,  B01D61/027

Abstract: Methods and systems for increasing the yield and/or throughput of a product produced in a multi-step equilibrium pathway are described. Product yield and/or throughput is increased through supersaturation of a solubility-limited substrate. Supersaturation may be achieved, in some embodiments, through membrane dewatering. In some embodiments, the product is monatin and the substrate is tryptophan.

Abstract translation: 描述了用于增加在多步平衡通路中产生的产物的产量和/或产量的方法和系统。 通过溶解度限制底物的过饱和来提高产物产率和/或产量。 在一些实施方案中，可以通过膜脱水实现过饱和。 在一些实施方案中，产物是莫纳甜，底物是色氨酸。

公开(公告)号：WO2012166406A1

公开(公告)日：2012-12-06

申请号：PCT/US2012/038766

申请日：2012-05-21

Applicant: CARGILL, INCORPORATED ,  PEMBLE, Trent H. ,  SOLHEID, Christopher ,  HILBERT, Brent H. ,  DICKEY, AnaLisa ,  JOHNSON, Dustin P. ,  PORTER, Michael A.

Inventor： PEMBLE, Trent H. ,  SOLHEID, Christopher ,  HILBERT, Brent H. ,  DICKEY, AnaLisa ,  JOHNSON, Dustin P. ,  PORTER, Michael A.

IPC: C12P17/10 ,  C12P17/16

CPC classification number: C12P17/10 ,  B01D61/025 ,  B01D61/027

Abstract: Methods and systems for increasing the yield and/or throughput of a product produced in a multi-step equilibrium pathway are described. Product yield and/or throughput is increased through supersaturation of a solubility-limited substrate. Supersaturation may be achieved, in some embodiments, through membrane dewatering. In some embodiments, the product is monatin and the substrate is tryptophan.

Abstract translation: 描述了用于提高在多步平衡路径中产生的产品的产量和/或产量的方法和系统。 通过溶解度受限的底物的过饱和提高产物收率和/或产量。 在一些实施方式中，可以通过膜脱水来实现过饱和。 在一些实施方案中，产物是莫纳甜并且底物是色氨酸。