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    AN IMPROVED IN VITRO METHOD OF CULTURING MAMMALIAN CELLS FOR AUTOLOGOUS CELL IMPLANTATION/TRANSPLANTATION METHODS
    1.
    发明申请
    AN IMPROVED IN VITRO METHOD OF CULTURING MAMMALIAN CELLS FOR AUTOLOGOUS CELL IMPLANTATION/TRANSPLANTATION METHODS 审中-公开
    改进用于自体细胞植入/移植方法的培养MAMMALIAN细胞的体外培养方法

    公开(公告)号：WO02061052A8

    公开(公告)日：2004-04-15

    申请号：PCT/DK0200065

    申请日：2002-01-29

    Applicant: INTERFACE BIOTECH AS ,  STORGAARD PETER ,  OSTHER KURT

    Inventor： STORGAARD PETER ,  OSTHER KURT

    IPC: G01N33/68 ,  A61K20060101 ,  A61K35/12 ,  A61P1/16 ,  A61P1/18 ,  A61P3/10 ,  A61P9/00 ,  A61P17/00 ,  A61P17/02 ,  A61P19/00 ,  A61P19/02 ,  A61P19/08 ,  A61P19/10 ,  A61P25/00 ,  A61P25/16 ,  A61P25/28 ,  A61P27/02 ,  A61P35/00 ,  C12N20060101 ,  C12N5/00 ,  C12N5/077 ,  C12N5/0775 ,  C12Q1/02

    CPC classification number: C12N5/0655 ,  C12N5/0668 ,  C12N2500/60 ,  C12N2509/00 ,  C12N2523/00

    Abstract: A production method for producing cell colony forming units in vitro from a mammalian tissue explant, the method comprises the steps of a) growing a piece of the mammalian tissue explant in a growth medium to obtain cell colony forming units from immature cells from the piece of explant, and b) harvesting cells from one or more of the cell colony forming units for use in Autologous Cell Implantation/transplantation methods. The mammalian tissue explant is selected from the group consisting of cartilage; bone such as, e.g., bone marrow; connective tissue; muscle tissue such as, e.g., smooth muscle tissue, heart tissue, liver tissue and skeletal muscle tissue; skin tissue such as, e.g., periosteum; mucosal tissue; brain tissue, pancreas tissue and blood vessels. In particularly, the mammalian tissue explant is cartilage, such as elastic, fibro, hyalin or articular hyalin cartilage. The cells obtained are suitable for use in autologous implantation/transplantation methods. In a specific embodiment, the cell obtained are chondroytes, especially for use in autologous chondrocyte implantation (ACI) methods.

    Abstract translation: 一种用于从哺乳动物组织外植体体外产生细胞集落形成单位的制备方法，所述方法包括以下步骤：a）在生长培养基中培养一片哺乳动物组织外植体，以从未成熟细胞获得细胞集落形成单位 外植体，和b）从用于自体细胞植入/移植方法的一个或多个细胞集落形成单位收获细胞。 哺乳动物组织外植体选自软骨; 骨，例如骨髓; 结缔组织; 肌肉组织，例如平滑肌组织，心脏组织，肝组织和骨骼肌组织; 皮肤组织，例如骨膜; 粘膜组织; 脑组织，胰腺组织和血管。 特别地，哺乳动物组织外植体是软骨，例如弹性，纤维，透明质或关节透明质软骨。 获得的细胞适合用于自体植入/移植方法。 在具体实施方案中，所获得的细胞是软骨细胞，特别是用于自体软骨细胞植入（ACI）方法。

    AN IMPROVED IN VITRO METHOD OF CULTURING MAMMALIAN CELLS FOR AUTOLOGOUS CELL IMPLANTATION/TRANSPLANTATION METHODS
    2.
    发明申请
    AN IMPROVED IN VITRO METHOD OF CULTURING MAMMALIAN CELLS FOR AUTOLOGOUS CELL IMPLANTATION/TRANSPLANTATION METHODS 审中-公开

    公开(公告)号：WO2002061052A3

    公开(公告)日：2002-08-08

    申请号：PCT/DK2002/000065

    申请日：2002-01-29

    Applicant: INTERFACE BIOTECH A/S ,  STORGAARD, Peter ,  OSTHER, Kurt

    Inventor： STORGAARD, Peter ,  OSTHER, Kurt

    IPC: C12N5/00

    Abstract: A production method for producing cell colony forming units in vitro from a mammalian tissue explant, the method comprises the steps of a) growing a piece of the mammalian tissue explant in a growth medium to obtain cell colony forming units from immature cells from the piece of explant, and b) harvesting cells from one or more of the cell colony forming units for use in Autologous Cell Implantation/transplantation methods. The mammalian tissue explant is selected from the group consisting of cartilage; bone such as, e.g., bone marrow; connective tissue; muscle tissue such as, e.g., smooth muscle tissue, heart tissue, liver tissue and skeletal muscle tissue; skin tissue such as, e.g., periosteum; mucosal tissue; brain tissue, pancreas tissue and blood vessels. In particularly, the mammalian tissue explant is cartilage, such as elastic, fibro, hyalin or articular hyalin cartilage. The cells obtained are suitable for use in autologous implantation/transplantation methods. In a specific embodiment, the cell obtained are chondroytes, especially for use in autologous chondrocyte implantation (ACI) methods.

    AN IMPROVED IN VITRO METHOD OF CULTURING MAMMALIAN CELLS FOR AUTOLOGOUS CELL IMPLANTATION/TRANSPLANTATION METHODS
    3.
    发明申请
    AN IMPROVED IN VITRO METHOD OF CULTURING MAMMALIAN CELLS FOR AUTOLOGOUS CELL IMPLANTATION/TRANSPLANTATION METHODS 审中-公开
    改进用于自体细胞植入/移植方法的培养MAMMALIAN细胞的体外培养方法

    公开(公告)号：WO2002061052A2

    公开(公告)日：2002-08-08

    申请号：PCT/DK2002/000065

    申请日：2002-01-29

    Applicant: INTERFACE BIOTECH A/S ,  STORGAARD, Peter ,  OSTHER, Kurt

    Inventor： STORGAARD, Peter ,  OSTHER, Kurt

    IPC: C12N5/00

    CPC classification number: C12N5/0655 ,  C12N5/0668 ,  C12N2500/60 ,  C12N2509/00 ,  C12N2523/00

    Abstract: A production method for producing cell colony forming units in vitro from a mammalian tissue explant, the method comprises the steps of a) growing a piece of the mammalian tissue explant in a growth medium to obtain cell colony forming units from immature cells from the piece of explant, and b) harvesting cells from one or more of the cell colony forming units for use in Autologous Cell Implantation/transplantation methods. The mammalian tissue explant is selected from the group consisting of cartilage; bone such as, e.g., bone marrow; connective tissue; muscle tissue such as, e.g., smooth muscle tissue, heart tissue, liver tissue and skeletal muscle tissue; skin tissue such as, e.g., periosteum; mucosal tissue; brain tissue, pancreas tissue and blood vessels. In particularly, the mammalian tissue explant is cartilage, such as elastic, fibro, hyalin or articular hyalin cartilage. The cells obtained are suitable for use in autologous implantation/transplantation methods. In a specific embodiment, the cell obtained are chondroytes, especially for use in autologous chondrocyte implantation (ACI) methods.

    Abstract translation: 一种用于从哺乳动物组织外植体体外产生细胞集落形成单元的生产方法，所述方法包括以下步骤：a）在生长培养基中培养一片哺乳动物组织外植体以获得细胞集落形成单位 来自所述外植体的未成熟细胞，以及b）从用于自体细胞植入/移植方法的一个或多个细胞集落形成单位收获细胞。 哺乳动物组织外植体选自软骨; 骨，例如骨髓; 结缔组织; 肌肉组织，例如平滑肌组织，心脏组织，肝组织和骨骼肌组织; 皮肤组织，例如骨膜; 粘膜组织; 脑组织，胰腺组织和血管。 特别地，哺乳动物组织外植体是软骨，例如弹性，纤维，透明质或关节透明质软骨。 获得的细胞适合用于自体植入/移植方法。 在具体实施方案中，所获得的细胞是软骨细胞，特别是用于自体软骨细胞植入（ACI）方法。

    ARTHROSCOPIC METHOD FOR CELL IMPLANTATION IN MAMMALS
    6.
    发明申请
    ARTHROSCOPIC METHOD FOR CELL IMPLANTATION IN MAMMALS 审中-公开
    用于细胞植入的神经元方法

    公开(公告)号：WO2005079881A1

    公开(公告)日：2005-09-01

    申请号：PCT/DK2005/000107

    申请日：2005-02-18

    Applicant: INTERFACE BIOTECH A/S ,  STORGAARD, Peter

    Inventor： STORGAARD, Peter

    IPC: A61L27/38

    CPC classification number: A61L27/3886 ,  A61B1/317 ,  A61B17/3468 ,  A61K35/32 ,  A61L27/3817 ,  A61L27/3821 ,  A61L27/3847 ,  A61L27/3852 ,  A61L27/3895 ,  A61L27/54 ,  A61L2300/252 ,  A61L2300/442 ,  C12N5/0655 ,  C12N2501/70

    Abstract: The present invention relates to an endoscopic method for treating cartilage or bone defects in a mammal, said method comprising the steps of: i.-dentifying the position of the defect, ii.-applying a cell suspension comprising cells selected from the group consisting of chondrocytes, chondroblasts, osteocytes and osteoblasts and combinations thereof into the cartilage or bone defect, the defect being positioned in such a manner that the cell suspension substantially does not flow out of the defect upon application.

    Abstract translation: 本发明涉及一种用于治疗哺乳动物软骨或骨缺损的内窥镜检查方法，所述方法包括以下步骤：i。 - 修复缺损位置，ii。 - 施用包含选自下组的细胞的细胞悬液： 软骨细胞，软骨细胞，骨细胞和成骨细胞及其组合进入软骨或骨缺损，所述缺陷以使得细胞悬浮液在施用时基本上不会流出缺陷的方式定位。

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