公开(公告)号：WO2009074524A2

公开(公告)日：2009-06-18

申请号：PCT/EP2008066977

申请日：2008-12-08

Applicant: BASF SE ,  STUERMER RAINER ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  FABER KURT ,  HALL MELANIE ,  MACHEROUX PETER ,  STUECKLER CLEMENS

Inventor： STUERMER RAINER ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  FABER KURT ,  HALL MELANIE ,  MACHEROUX PETER ,  STUECKLER CLEMENS

IPC: C12P13/04

CPC classification number: C12P13/04

Abstract: The invention relates to a method for production of amino acids of general formula (3) or (4) from alpha-dehydroamino acids of general formula (1) or (2), where R1 and R2 independently = H, C1-C6 alkyl, C2-C6 alkenyl, optionally substituted carbo- or heterocyclic, aromatic or non-aromatic or alkylaryl, or a carboxyl ( -COOR) group, R3 = H, formyl, acetyl, propionyl, benzyl, benzyloxycarbonyl, BOC, or Alloc and R = H, C1-C6 alkyl or aryl, by reduction of a compound of formula (1) or (2) in the presence of a reductase.

Abstract translation: 工艺的通式（3）或（4）通式（1）或由α-脱氢酸（2）其中R1，R2独立地为H，C1-C6烷基，C2-C6链烯基的氨基酸的酶制剂 ，任选取代的碳环或杂环芳族或非芳族基团，或一个Alkylaryrest，或羧基（ - COOR），R 3是H，甲酰基，乙酰基，丙酰基，苄基，苄氧羰基，BOC，的Alloc，R为H，C1 -C6-烷基，芳基，通过还原式（1）或（2）在一个还原酶的存在。

公开(公告)号：WO2010139651A3

公开(公告)日：2011-03-03

申请号：PCT/EP2010057511

申请日：2010-05-31

Applicant: BASF SE ,  MAURER STEFFEN ,  HAUER BERNHARD ,  BONNEKESSEL MELANIE ,  FABER KURT ,  STUECKLER CLEMENS

Inventor： MAURER STEFFEN ,  HAUER BERNHARD ,  BONNEKESSEL MELANIE ,  FABER KURT ,  STUECKLER CLEMENS

IPC: C12P7/24 ,  C12N9/02 ,  C12P7/26 ,  C12P7/40 ,  C12P7/62

CPC classification number: C12P7/24 ,  C12N9/001 ,  C12P7/22 ,  C12P7/26 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/62 ,  C12P17/10 ,  C12Y103/01031

Abstract: A process for the enzymatic reduction of an enoate (1) wherein the C=C bond of the enoate (1) is stereoselectively hydrogenated in the presence of an enoate-reductase and an oxidizable co-substrate (2) in a system which is free of NAD(P)H, a. formula (1); b. in which c. A is a ketone radical (-CRO), an aldehyde radical (-CHO), a carboxyl radical (-COOR), with R = H or optionally substituted C1-C6-alkyl radical, d. R1, R2 and R3 are independently of one another H, -O-C1-C6-alkyl, -O-W with W = a hydroxyl protecting group, C1-C6-alkyl, which can be substituted, C2-C6-alkenyl, carboxyl, or an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or one of R1, R2 and R3 is a -OH radical, or R1 is linked to R3 so as to become part of a 4-8-membered cycle, or R1 is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R1, R2 and R3 may not be identical.

Abstract translation: 在酸酐（1）的酶还原方法中，其中在酸酐还原酶和可氧化共底物（2）的存在下，在无自由基的体系中立体选择性地氢化了enoate（1）的C = C键 的NAD（P）H，a。 公式1）; 湾 其中c。 A是酮基（-CRO），醛基（-CHO），羧基（-COOR），其中R = H或任选取代的C 1 -C 6 - 烷基，d。 R 1，R 2和R 3彼此独立地为H，-O-C 1 -C 6烷基， - W，其中W =羟基保护基，可被取代的C 1 -C 6 - 烷基，C 2 -C 6 - 烯基，羧基， 或任选取代的碳 - 或杂环芳族或非芳族基团，或R 1，R 2和R 3中的一个为-OH基团，或R 1与R 3连接以成为4-8元环的一部分，或R 1 与R连接成为4-8元环的一部分，条件是R1，R2和R3可能不相同。