公开(公告)号：US07619061B2

公开(公告)日：2009-11-17

申请号：US11008351

申请日：2004-12-09

Applicant: John R. Hackett, Jr. ,  Julie Yamaguchi ,  Alan M. Golden ,  Catherine A. Brennan ,  Robert K. Hickman ,  Sushil G. Devare

Inventor： John R. Hackett, Jr. ,  Julie Yamaguchi ,  Alan M. Golden ,  Catherine A. Brennan ,  Robert K. Hickman ,  Sushil G. Devare

IPC: C07K14/16

CPC classification number: C07K14/005 ,  A61K39/00 ,  C07K2319/00 ,  C12N2740/16122 ,  G01N2333/162

Abstract: Isolated HIV-1 Group O env polypeptides obtained from the HIV-1 isolate HAM112 are claimed, as well as (a) antigen constructs comprising fusions of one or more of each of HIV-1 Group O env polypeptides and HIV-1 Group M env polypeptide and (b) further antigen constructs containing additional Group O sequences and especially the gp41 IDR of isolate HAM112. Also claimed are polynucleotide sequences encoding the above, expression vectors comprising the same, host cells transformed thereby, and immunoassay methods and kits utilizing the antigen constructs of the invention.

Abstract translation: 要求保护从HIV-1分离株HAM112获得的分离的HIV-1组O env多肽，以及（a）包含HIV-1组O型多肽和HIV-1组M env中的一种或多种的融合物的抗原构建体 多肽和（b）另外的含有其他组O序列的抗原构建物，特别是分离株HAM112的gp41 IDR。 还要求保护的是编码上述的多核苷酸序列，包含其的表达载体，由此转化的宿主细胞，以及利用本发明的抗原构建体的免疫测定方法和试剂盒。

公开(公告)号：US20090280474A1

公开(公告)日：2009-11-12

申请号：US12117550

申请日：2008-05-08

Applicant: Lynn A. Martin ,  Sushil G. Devare ,  Mary C. Kuhns

Inventor： Lynn A. Martin ,  Sushil G. Devare ,  Mary C. Kuhns

IPC: C12Q1/70

CPC classification number: G01N33/576 ,  G01N33/5306 ,  G01N2333/02 ,  G01N2333/186

Abstract: This invention is related a method for increasing the sensitivity of detecting a viral target in a sample. The sensitivity may be increased by disrupting a complex comprising the target or by measuring the level of the target from a larger volume of the sample.

Abstract translation: 本发明涉及增加样品中病毒靶标检测灵敏度的方法。 可以通过破坏包含靶的复合物或通过从较大体积的样品测量靶标的水平来增加灵敏度。

公开(公告)号：US20100035237A1

公开(公告)日：2010-02-11

申请号：US12574407

申请日：2009-10-06

Applicant: John R. Hackett, JR. ,  Julie Yamaguchi ,  Alan M. Golden ,  Catherine A. Brennan ,  Robert K. Hickman ,  Sushil G. Devare

Inventor： John R. Hackett, JR. ,  Julie Yamaguchi ,  Alan M. Golden ,  Catherine A. Brennan ,  Robert K. Hickman ,  Sushil G. Devare

IPC: C12Q1/70

CPC classification number: C07K14/005 ,  A61K39/00 ,  C07K2319/00 ,  C12N2740/16122 ,  G01N2333/162

Abstract: Isolated HIV-1 Group O env polypeptides obtained from the HIV-1 isolate HAM112 are claimed, as well as (a) antigen constructs comprising fusions of one or more of each of HIV-1 Group O env polypeptides and HIV-1 Group M env polypeptide and (b) further antigen constructs containing additional Group O sequences and especially the gp41 IDR of isolate HAM112. Also claimed are polynucleotide sequences encoding the above, expression vectors comprising the same, host cells transformed thereby, and immunoassay methods and kits utilizing the antigen constructs of the invention.

Abstract translation: 要求保护从HIV-1分离株HAM112获得的分离的HIV-1组O env多肽，以及（a）包含HIV-1组O型多肽和HIV-1组M env中的一种或多种的融合物的抗原构建体 多肽和（b）另外的含有其他组O序列的抗原构建物，特别是分离株HAM112的gp41 IDR。 还要求保护的是编码上述的多核苷酸序列，包含其的表达载体，由此转化的宿主细胞，以及利用本发明的抗原构建体的免疫测定方法和试剂盒。

公开(公告)号：US5922533A

公开(公告)日：1999-07-13

申请号：US912129

申请日：1997-08-15

Applicant: Anadruzela S. Vallari ,  John R. Hackett, Jr. ,  Robert K. Hickman ,  Vincent A. Varitek, Jr. ,  Elizabeth C. Necklaws ,  Alan M. Golden ,  Catherine A. Brennan ,  Sushil G. Devare

Inventor： Anadruzela S. Vallari ,  John R. Hackett, Jr. ,  Robert K. Hickman ,  Vincent A. Varitek, Jr. ,  Elizabeth C. Necklaws ,  Alan M. Golden ,  Catherine A. Brennan ,  Sushil G. Devare

IPC: C12Q1/68 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/70

CPC classification number: G01N33/56988 ,  G01N33/54386 ,  Y10S435/948 ,  Y10S435/974 ,  Y10S435/975 ,  Y10S530/826

Abstract: A method of performing a rapid assay for the simultaneous detection and differentiation of the analytes HIV-1 group M. HIV-1 group O and HIV-2 utilizing a sequence specific polypeptide of each analyte as capture reagents. An analytical. device also is provided for performing the method which includes these capture reagents. Also provided is a test kit which includes the analytical device which further can include a positive and negative control.

Abstract translation: 使用每种分析物的序列特异性多肽同时检测和分化HIV-1组M-HIV-1组O和HIV-2的快速测定方法作为捕获试剂。 还提供了用于执行包括这些捕获试剂的方法的分析装置。 还提供了一种测试试剂盒，其包括进一步可以包括阳性和阴性对照的分析装置。

公开(公告)号：US5859193A

公开(公告)日：1999-01-12

申请号：US314570

申请日：1994-09-29

Applicant: Sushil G. Devare ,  James M. Casey ,  Suresh M. Desai

Inventor： Sushil G. Devare ,  James M. Casey ,  Suresh M. Desai

IPC: G01N33/569 ,  A61K39/21 ,  C07K1/00 ,  C07K14/00 ,  C07K14/155 ,  C07K14/16 ,  C07K14/195 ,  C07K14/41 ,  C07K14/705 ,  C07K19/00 ,  C12N9/00 ,  C12N15/09 ,  C12N15/48 ,  C12P21/02 ,  C12R1/19 ,  C12N1/20 ,  G01N33/53

CPC classification number: C07K14/005 ,  C07K2319/00 ,  C07K2319/31 ,  C07K2319/40 ,  C12N2740/16122

Abstract: The present invention provides a method of synthesizing genes encoding unique HIV-1 and HIV-2 envelope proteins and their fragments, thereby allowing overexpression of these proteins in E. coli. The HIV envelope proteins and their fragments have been expressed at high levels as individual proteins or in fusion with other proteins. The HIV envelope proteins thus expressed in E. coli can be effectively used for the detection of exposure to HIV as well as the discrimination of HIV-1 and HIV-2.

公开(公告)号：US06538127B1

公开(公告)日：2003-03-25

申请号：US08443530

申请日：1995-05-18

Applicant: Sushil G. Devare ,  James M. Casey ,  Suresh M. Desai

Inventor： Sushil G. Devare ,  James M. Casey ,  Suresh M. Desai

IPC: C07H2104

CPC classification number: C07K14/005 ,  C07K2319/00 ,  C07K2319/31 ,  C07K2319/40 ,  C12N2740/16122

Abstract: The present invention provides a method of synthesizing genes encoding unique HIV-1 and HIV-2 envelope proteins and their fragments, thereby allowing overexpression of these proteins in E. coli. The HIV envelope proteins and their fragments have been expressed at high levels as individual proteins or in fusion with other proteins. The HIV envelope proteins thus expressed in E. coli can be effectively used for the detection of exposure to HIV as well as the discrimination of HIV-1 and HIV-2.

Abstract translation: 本发明提供了合成编码唯一HIV-1和HIV-2包膜蛋白及其片段的基因的方法，由此允许这些蛋白质在大肠杆菌中的过表达。 HIV包膜蛋白及其片段已被高度表达为单个蛋白质或与其他蛋白质融合。 因此在大肠杆菌中表达的HIV包膜蛋白可以有效地用于检测HIV的暴露以及HIV-1和HIV-2的区别。

公开(公告)号：US6153377A

公开(公告)日：2000-11-28

申请号：US443961

申请日：1995-05-18

Applicant: Sushil G. Devare ,  James M. Casey ,  Suresh M. Desai

Inventor： Sushil G. Devare ,  James M. Casey ,  Suresh M. Desai

IPC: G01N33/569 ,  A61K39/21 ,  C07K1/00 ,  C07K14/00 ,  C07K14/155 ,  C07K14/16 ,  C07K14/195 ,  C07K14/41 ,  C07K14/705 ,  C07K19/00 ,  C12N9/00 ,  C12N15/09 ,  C12N15/48 ,  C12P21/02 ,  C12R1/19 ,  C12Q1/70

CPC classification number: C07K14/005 ,  C07K2319/00 ,  C07K2319/31 ,  C07K2319/40 ,  C12N2740/16122

Abstract: The present invention provides a method of synthesizing genes encoding unique HIV-1 and HIV-2 envelope proteins and their fragments, thereby allowing overexpression of these proteins in E. coli. The HIV envelope proteins and their fragments have been expressed at high levels as individual proteins or in fusion with other proteins. The HIV envelope proteins thus expressed in E. coli can be effectively used for the detection of exposure to HIV as well as the discrimination of HIV-1 and HIV-2.

Abstract translation: 本发明提供了合成编码唯一HIV-1和HIV-2包膜蛋白及其片段的基因的方法，由此允许这些蛋白质在大肠杆菌中的过表达。 HIV包膜蛋白及其片段已被高度表达为单个蛋白质或与其他蛋白质融合。 因此在大肠杆菌中表达的HIV包膜蛋白可以有效地用于检测HIV的暴露以及HIV-1和HIV-2的区别。

公开(公告)号：US5667992A

公开(公告)日：1997-09-16

申请号：US453552

申请日：1995-05-30

Applicant: James M. Casey ,  Suzanne L. Bode ,  Billy J. Zeck ,  Julie Yamaguchi ,  Donald E. Frail ,  Suresh M. Desai ,  Sushil G. Devare

Inventor： James M. Casey ,  Suzanne L. Bode ,  Billy J. Zeck ,  Julie Yamaguchi ,  Donald E. Frail ,  Suresh M. Desai ,  Sushil G. Devare

IPC: C12N15/09 ,  A61K38/00 ,  C07K14/18 ,  C07K16/00 ,  C07K16/10 ,  C07K19/00 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/70 ,  C12R1/91 ,  C12P21/00

CPC classification number: C07K14/005 ,  A61K38/00 ,  C12N2770/24222

Abstract: Mammalian expression systems for the production of HCV proteins. Such expression systems provide high yields of HCV proteins, and enable the development of diagnostic and therapeutic reagents which contain glycosylated structural antigens and also allow for the isolation of the HCV etiological agent.

Abstract translation: 用于生产HCV蛋白的哺乳动物表达系统。 这种表达系统提供高产量的HCV蛋白质，并且能够开发含有糖基化结构抗原的诊断和治疗试剂，并且还允许分离HCV病原体。

公开(公告)号：US5610009A

公开(公告)日：1997-03-11

申请号：US188281

申请日：1994-01-28

Applicant: Shinichi Watanabe ,  Julie Yamaguchi ,  Suresh M. Desai ,  Sushil G. Devare

Inventor： Shinichi Watanabe ,  Julie Yamaguchi ,  Suresh M. Desai ,  Sushil G. Devare

IPC: G01N33/576 ,  A61K39/00 ,  A61K39/29 ,  A61K39/395 ,  A61P31/12 ,  C07H21/04 ,  C07K14/18 ,  C07K14/47 ,  C07K16/08 ,  C07K19/00 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/70

CPC classification number: C07K14/005 ,  C07K14/4711 ,  A61K39/00 ,  C07K2319/00 ,  C12N2770/24222 ,  Y10S436/82

Abstract: Mammalian expression systems for the production of HCV E1-E2 fusion proteins. Such expression systems provide high yields of HCV proteins extracelluarly, and enable the development of diagnostic, vaccine and therapeutic reagents which contain glycosylated structural antigens and also allow for the isolation of the HCV etiological agent.

Abstract translation: 用于生产HCV E1-E2融合蛋白的哺乳动物表达系统。 这种表达系统提供高产量的HCV蛋白质，并且能够开发含有糖基化结构抗原的诊断，疫苗和治疗试剂，并且还允许HCV病原体的分离。

公开(公告)号：US5308750A

公开(公告)日：1994-05-03

申请号：US748292

申请日：1991-08-21

Applicant: Smriti U. Mehta ,  Jill E. Johnson ,  Stephen H. Dailey ,  Suresh M. Desai ,  Sushil G. Devare

Inventor： Smriti U. Mehta ,  Jill E. Johnson ,  Stephen H. Dailey ,  Suresh M. Desai ,  Sushil G. Devare

IPC: C12Q1/70 ,  C07K7/00 ,  C07K14/02 ,  C07K14/10 ,  C07K14/18 ,  C07K16/00 ,  C07K16/10 ,  C12N5/10 ,  C12N5/20 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/53 ,  G01N33/569 ,  G01N33/576 ,  G01N33/577 ,  C12N5/00

CPC classification number: C07K14/005 ,  C07K16/109 ,  G01N33/5767 ,  C07K2317/34 ,  C12N2770/24222 ,  Y10S436/82

Abstract: Monoclonal antibodies which specifically bind to Hepatitis C Virus (HCV) E2/NS1 antigen. Also provided are hybridoma cell lines which secrete these monoclonal antibodies, methods for using these monoclonal antibodies, and assay kits for assays which contain these monoclonal antibodies.

Abstract translation: 特异性结合丙型肝炎病毒（HCV）E2 / NS1抗原的单克隆抗体。 还提供了分泌这些单克隆抗体的杂交瘤细胞系，使用这些单克隆抗体的方法和用于含有这些单克隆抗体的测定的测定试剂盒。