公开(公告)号：WO1997043901A1

公开(公告)日：1997-11-27

申请号：PCT/US1997008558

申请日：1997-05-20

Applicant: VANDERBILT UNIVERSITY ,  MILLER, Geraldine, G. ,  PEEK, Richard, M., Jr. ,  THOMPSON, Stuart, A. ,  BLASER, Martin, J.

Inventor： VANDERBILT UNIVERSITY

IPC: A01N43/04

CPC classification number: C07K14/205 ,  A61K38/00 ,  A61K39/00

Abstract: A purified IceA protein of Helicobacter pylori is provided. The protein is expressed as either an IceA 1 or an IceA 2 variant. A purified polypeptide fragment of the IceA protein is also provided. An antigenic fragment of IceA is provided. An isolated nucleic acid that encodes an IceA protein of H. pylori is provided. A nucleic acid that encodes an IceA 1 variant and a nucleic acid that encodes an IceA 2 variant is also provided. Fragments of the IceA gene are provided. A method of detecting the presence of an antibody against H. pylori in a sample is provided. The method comprises the following steps: a) contacting the sample with a purified IceA protein of H. pylori or a H. pylori-specific fragment thereof; and b) detecting the binding of the antibody in the sample to the protein or fragment, the detection of biding indicating the presence in the sample of antibodies against H. pylori. A method of detecting the presence of an antibody against an ulcerative Helicobacter pylori strain in a sample is also provided.

Abstract translation: 提供幽门螺杆菌纯化的IceA蛋白。 蛋白质表达为IceA 1或IceA 2变体。 还提供了IceA蛋白的纯化多肽片段。 提供了IceA的抗原片段。 提供编码幽门螺杆菌的IceA蛋白的分离的核酸。 还提供了编码IceA 1变体的核酸和编码IceA 2变体的核酸。 提供了IceA基因的片段。 提供了检测样品中幽门螺杆菌抗体的存在的方法。 该方法包括以下步骤：a）将样品与幽门螺杆菌的纯化IceA蛋白或幽门螺杆菌特异性片段接触; 和b）检测样品中的抗体与蛋白质或片段的结合，检测指示在幽门螺杆菌抗体样品中的存在。 还提供了检测样品中针对溃疡性幽门螺杆菌菌株的抗体存在的方法。

公开(公告)号：WO9743901A9

公开(公告)日：2001-06-21

申请号：PCT/US9708558

申请日：1997-05-20

Applicant: UNIV VANDERBILT ,  MILLER GERALDINE G ,  PEEK RICHARD M JR ,  THOMPSON STUART A ,  BLASER MARTIN J

Inventor： MILLER GERALDINE G ,  PEEK RICHARD M JR ,  THOMPSON STUART A ,  BLASER MARTIN J

IPC: G01N33/53 ,  A61K31/00 ,  A61K38/00 ,  A61K39/00 ,  A61K39/106 ,  A61K39/395 ,  A61P31/00 ,  A61P31/04 ,  C07K14/205 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/68 ,  C12R1/01 ,  G01N33/569 ,  A01N43/04 ,  A61K31/70 ,  A61K39/38 ,  C07K1/00 ,  C12P21/06

CPC classification number: C07K14/205 ,  A61K38/00 ,  A61K39/00

Abstract: A purified IceA protein of Helicobacter pylori is provided. The protein is expressed as either an IceA 1 or an IceA 2 variant. A purified polypeptide fragment of the IceA protein is also provided. An antigenic fragment of IceA is provided. An isolated nucleic acid that encodes an IceA protein of H. pylori is provided. A nucleic acid that encodes an IceA 1 variant and a nucleic acid that encodes an IceA 2 variant is also provided. Fragments of the IceA gene are provided. A method of detecting the presence of an antibody against H. pylori in a sample is provided. The method comprises the following steps: a) contacting the sample with a purified IceA protein of H. pylori or a H. pylori-specific fragment thereof; and b) detecting the binding of the antibody in the sample to the protein or fragment, the detection of biding indicating the presence in the sample of antibodies against H. pylori. A method of detecting the presence of an antibody against an ulcerative Helicobacter pylori strain in a sample is also provided.

Abstract translation: 提供幽门螺杆菌纯化的IceA蛋白。 蛋白质表达为IceA 1或IceA 2变体。 还提供了IceA蛋白的纯化多肽片段。 提供了IceA的抗原片段。 提供编码幽门螺杆菌的IceA蛋白的分离的核酸。 还提供了编码IceA 1变体的核酸和编码IceA 2变体的核酸。 提供了IceA基因的片段。 提供了检测样品中幽门螺杆菌抗体的存在的方法。 该方法包括以下步骤：a）将样品与幽门螺杆菌的纯化IceA蛋白或幽门螺杆菌特异性片段接触; 和b）检测样品中的抗体与蛋白质或片段的结合，检测指示在幽门螺杆菌抗体样品中的存在。 还提供了检测样品中针对溃疡性幽门螺杆菌菌株的抗体存在的方法。