公开(公告)号：WO2006138261A2

公开(公告)日：2006-12-28

申请号：PCT/US2006/022908

申请日：2006-06-12

Applicant: STRATAGENE CALIFORNIA ,  REID, Taylor, A. ,  TAYLOR, Roger, H. ,  ZAJAC, Kenneth, J.

Inventor： REID, Taylor, A. ,  TAYLOR, Roger, H. ,  ZAJAC, Kenneth, J.

IPC: E03D11/00

CPC classification number: G01J3/10 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6484 ,  G01N2201/0446 ,  G01N2201/0612 ,  G01N2201/062

Abstract: A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source(40) that emits an excitation light (48) into an illumination tube (44); a plurality of collection optics (39) located around an aperture (47) in the illumination tube for collecting fluorescence; and a detector (53) for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light (48) from a light source (40) into an illumination tube (44); directing the excitation light to an excitation filter (62); illuminating a sample with the excitation light (48) to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics (39) located around the illumination tube (44).

Abstract translation: 公开了具有不同光路的用于荧光激发和检测的系统和方法。 用于检测荧光的系统包括将激发光（48）发射到照明管（44）中的光源（40）; 位于所述照明管中用于收集荧光的孔（47）周围的多个收集光学器件（39） 和用于确定荧光量的检测器（53）。 检测荧光的方法包括将光源（40）的激发光（48）发射到照明管（44）中; 将激发光引导到激发过滤器（62）; 用激发光（48）照射样品以产生发射光; 以及使用位于所述照明管（44）周围的多个收集光学器件（39）来检测所述发射光的光学特性。

公开(公告)号：WO2006138261A3

公开(公告)日：2007-09-07

申请号：PCT/US2006022908

申请日：2006-06-12

Applicant: STRATAGENE CALIFORNIA ,  REID TAYLOR A ,  TAYLOR ROGER H ,  ZAJAC KENNETH J

Inventor： REID TAYLOR A ,  TAYLOR ROGER H ,  ZAJAC KENNETH J

IPC: G01N21/01

CPC classification number: G01J3/10 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6484 ,  G01N2201/0446 ,  G01N2201/0612 ,  G01N2201/062

Abstract: A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source(40) that emits an excitation light (48) into an illumination tube (44); a plurality of collection optics (39) located around an aperture (47) in the illumination tube for collecting fluorescence; and a detector (53) for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light (48) from a light source (40) into an illumination tube (44); directing the excitation light to an excitation filter (62); illuminating a sample with the excitation light (48) to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics (39) located around the illumination tube (44).

Abstract translation: 公开了具有不同光路的用于荧光激发和检测的系统和方法。 用于检测荧光的系统包括将激发光（48）发射到照明管（44）中的光源（40）; 位于所述照明管中用于收集荧光的孔（47）周围的多个收集光学器件（39） 和用于确定荧光量的检测器（53）。 检测荧光的方法包括将光源（40）的激发光（48）发射到照明管（44）中; 将激发光引导到激发过滤器（62）; 用激发光（48）照射样品以产生发射光; 以及使用位于所述照明管（44）周围的多个收集光学器件（39）来检测所述发射光的光学特性。

公开(公告)号：WO2004031342A1

公开(公告)日：2004-04-15

申请号：PCT/US2003/031194

申请日：2003-10-01

Applicant: STRATAGENE ,  BROWN, Larry, R. ,  BRUMLEY, William, D. ,  ZAJAC, Kenneth, J.

Inventor： BROWN, Larry, R. ,  BRUMLEY, William, D. ,  ZAJAC, Kenneth, J.

IPC: C12M1/00

CPC classification number: B01L3/50851 ,  B01L3/50853 ,  B01L7/52 ,  B01L7/54 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844

Abstract: A flexible heating cover assembly (200) for an apparatus for heating samples of biological material with substantial temperature uniformity includes a housing having a plurality of engageable enclosure components; a resistive heater (300) having a plurality of heater element areas; a heater backing plate (350) providing stability to the resistive heater (300); a force distribution system that distributes a force over the heater backing plate (350); and a support plate (500) providing stiffness for the force distribution system, wherein the arrangement of the resistive heater (300), the heater backing plate (350), the force distribution system and the support plate (500) provide substantial temperature uniformity among a plurality of sample tubes (140) for receiving samples of biological material. The flexible heating cover assembly (200) improves the uniformity, efficiency, quality, reliability and controllability of the thermal response during thermal cycling of the biological material.

Abstract translation: 一种用于加热具有显着温度均匀性的生物材料样品的装置的柔性加热盖组件（200）包括具有多个可接合外壳部件的壳体; 具有多个加热元件区域的电阻加热器（300）; 加热器背板（350），其向所述电阻加热器（300）提供稳定性; 力分布系统，其在所述加热器背板（350）上分布力; 以及为力分配系统提供刚度的支撑板（500），其中电阻加热器（300），加热器背板（350），力分配系统和支撑板（500）的布置在 用于接收生物材料样品的多个样品管（140）。 柔性加热盖组件（200）改善了生物材料热循环期间的热响应的均匀性，效率，质量，可靠性和可控性。