公开(公告)号：WO2004048408A3

公开(公告)日：2004-07-29

申请号：PCT/NL0300831

申请日：2003-11-25

Applicant: AZ UNIV AMSTERDAM ,  PEPPELENBOSCH MAIKEL PETRUS ,  ZIVKOVIC DANICA ,  DIKS SANDER ,  BINK ROBERT JOZEF

Inventor： PEPPELENBOSCH MAIKEL PETRUS ,  ZIVKOVIC DANICA ,  DIKS SANDER ,  BINK ROBERT JOZEF

IPC: C07K14/47 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/0797 ,  C12N5/06 ,  C07K14/435 ,  C07K14/46

CPC classification number: C12N5/0623 ,  C07K14/47 ,  C12N2501/40 ,  C12N2501/41

Abstract: The present invention relates to methods for expansion of stem or progenitor cells. These methods rely on Asb-a polypeptides or nucleic acids to temporarily suppress differentiation of the cells, thus allowing proliferation and self-renewal of the stem or progenitor cells. Abs-a polypeptides and coding sequences define a class of polypeptides and nucleic acids that are both structurally and functionally highly conserved among vertebrates. Asb-a polypeptides contain 6 ankyrin repeats and a SOCS box that mediate the effect of the polypeptide on the regulation of specific subsets of genes involved in differentiation. The invention discloses various methods to increase the intracellular concentration of an Asb-a polypeptide for suppression of terminal differentiation of the stem or progenitor cells. The invention further relates to Asb-a polypeptides and nucleic acids, vectors and host cells for use in methods for their production and for use in the method for expansion of stem or progenitor cells, as well as to stem or progenitor cells containing exogenous Asb-a polypeptides and nucleic acids.

Abstract translation: 本发明涉及用于扩增干细胞或祖细胞的方法。 这些方法依赖于Asb-a多肽或核酸来暂时抑制细胞的分化，从而允许干细胞或祖细胞的增殖和自我更新。 Abs-多肽和编码序列限定了在脊椎动物中在结构和功能上高度保守的一类多肽和核酸。 Asb-a多肽含有6个锚蛋白重复序列​​和SOCS盒，其介导多肽对参与分化的基因特异性亚基的调控的影响。 本发明公开了增加用于抑制干细胞或祖细胞末端分化的Asb-a多肽的细胞内浓度的各种方法。 本发明进一步涉及Asb-a多肽和核酸，载体和宿主细胞，用于其生产方法和用于扩增干细胞或祖细胞的方法，以及含有外源Asb- 多肽和核酸。

公开(公告)号：WO2004048408A2

公开(公告)日：2004-06-10

申请号：PCT/NL2003/000831

申请日：2003-11-25

Applicant: ACADEMISCH ZIEKENHUIS BIJ DE UNIVERSITEIT VAN AMSTERDAM ,  PEPPELENBOSCH, Maikel, Petrus ,  ZIVKOVIC, Danica ,  DIKS, Sander ,  BINK, Robert, Jozef

Inventor： PEPPELENBOSCH, Maikel, Petrus ,  ZIVKOVIC, Danica ,  DIKS, Sander ,  BINK, Robert, Jozef

IPC: C07K14/46

CPC classification number: C12N5/0623 ,  C07K14/47 ,  C12N2501/40 ,  C12N2501/41

Abstract: The present invention relates to methods for expansion of stem or progenitor cells. These methods rely on Asb-a polypeptides or nucleic acids to temporarily suppress differentiation of the cells, thus allowing proliferation and self-renewal of the stem or progenitor cells. Abs-a polypeptides and coding sequences define a class of polypeptides and nucleic acids that are both structurally and functionally highly conserved among vertebrates. Asb-a polypeptides contain 6 ankyrin repeats and a SOCS box that mediate the effect of the polypeptide on the regulation of specific subsets of genes involved in differentiation. The invention discloses various methods to increase the intracellular concentration of an Asb-a polypeptide for suppression of terminal differentiation of the stem or progenitor cells. The invention further relates to Asb-a polypeptides and nucleic acids, vectors and host cells for use in methods for their production and for use in the method for expansion of stem or progenitor cells, as well as to stem or progenitor cells containing exogenous Asb-a polypeptides and nucleic acids.

Abstract translation: 本发明涉及用于扩增干细胞或祖细胞的方法。 这些方法依赖于Asb-a多肽或核酸来暂时抑制细胞的分化，从而允许干细胞或祖细胞的增殖和自我更新。 Abs-多肽和编码序列限定了在脊椎动物中在结构和功能上高度保守的一类多肽和核酸。 Asb-a多肽含有6个锚蛋白重复序列​​和SOCS盒，其介导多肽对参与分化的基因的特定亚群的调节的影响。 本发明公开了增加用于抑制干细胞或祖细胞末端分化的Asb-a多肽的细胞内浓度的各种方法。 本发明进一步涉及Asb-a多肽和核酸，载体和宿主细胞，用于其生产方法和用于扩增干细胞或祖细胞的方法，以及含有外源Asb- 多肽和核酸。

公开(公告)号：WO2004048408A8

公开(公告)日：2005-08-04

申请号：PCT/NL0300831

申请日：2003-11-25

Applicant: AZ UNIV AMSTERDAM ,  PEPPELENBOSCH MAIKEL PETRUS ,  ZIVKOVIC DANICA ,  DIKS SANDER ,  BINK ROBERT JOZEF

Inventor： PEPPELENBOSCH MAIKEL PETRUS ,  ZIVKOVIC DANICA ,  DIKS SANDER ,  BINK ROBERT JOZEF

IPC: C07K14/47 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/0797 ,  C12N5/06 ,  C07K14/435 ,  C07K14/46

CPC classification number: C12N5/0623 ,  C07K14/47 ,  C12N2501/40 ,  C12N2501/41

Abstract: The present invention relates to methods for expansion of stem or progenitor cells. These methods rely on Asb-a polypeptides or nucleic acids to temporarily suppress differentiation of the cells, thus allowing proliferation and self-renewal of the stem or progenitor cells. Abs-a polypeptides and coding sequences define a class of polypeptides and nucleic acids that are both structurally and functionally highly conserved among vertebrates. Asb-a polypeptides contain 6 ankyrin repeats and a SOCS box that mediate the effect of the polypeptide on the regulation of specific subsets of genes involved in differentiation. The invention discloses various methods to increase the intracellular concentration of an Asb-a polypeptide for suppression of terminal differentiation of the stem or progenitor cells. The invention further relates to Asb-a polypeptides and nucleic acids, vectors and host cells for use in methods for their production and for use in the method for expansion of stem or progenitor cells, as well as to stem or progenitor cells containing exogenous Asb-a polypeptides and nucleic acids.

Abstract translation: 本发明涉及用于扩增干细胞或祖细胞的方法。 这些方法依赖于Asb-a多肽或核酸来暂时抑制细胞的分化，从而允许干细胞或祖细胞的增殖和自我更新。 Abs-多肽和编码序列限定了在脊椎动物中在结构和功能上高度保守的一类多肽和核酸。 Asb-a多肽含有6个锚蛋白重复序列​​和SOCS盒，其介导多肽对参与分化的基因的特定亚群的调节的影响。 本发明公开了增加用于抑制干细胞或祖细胞末端分化的Asb-a多肽的细胞内浓度的各种方法。 本发明进一步涉及Asb-a多肽和核酸，载体和宿主细胞，用于其生产方法和用于扩增干细胞或祖细胞的方法，以及含有外源Asb- 多肽和核酸。