公开(公告)号：KR100485056B1

公开(公告)日：2005-04-22

申请号：KR1020040003621

申请日：2004-01-19

Applicant: (주)네추럴에프앤피

Inventor： 문원국 ,  김동우 ,  정봉현 ,  박정훈

IPC: A61K31/716

Abstract: Provided is an anti-H1N2 type viral composition comprising a soluble glucan oligomer of Saccharomyces cerevisiae. The soluble glucan oligomer of Saccharomyces cerevisiae is useful for inhibition of porcine influenza virus having gene fragments of bird influenza virus. The anti-H1N2 type viral composition comprises soluble glucan oligomer of Saccharomyces cerevisiae having molecular weight of 50,000 dalton or less as an effective component. The method for preparing the soluble glucan oligomer of Saccharomyces cerevisiae comprises the steps of: (a) culturing a Saccharomyces cerevisiae mutant IS2(KCTC 0959BP) in a broth medium for inoculation; (b) inoculating the cultured medium of Saccharomyces cerevisiae mutant IS2(KCTC 0959BP) into the broth medium, fed-batch culturing the microorganism and centrifuging the cultured medium to obtain Saccharomyces cerevisiae mutant IS2(KCTC 0959BP); (c) adding NaOH into the Saccharomyces cerevisiae mutant IS2(KCTC 0959BP) obtained to extract beta-glucan from the cellular membrane of the Saccharomyces cerevisiae mutant IS2(KCTC 0959BP); (d) reacting the extracted beta-glucan with a beta-glucanase, and filtering the reaction product to prepare the soluble glucan oligomer; and (e) freeze-drying the soluble glucan oligomer to obtain soluble glucan oligomer powder.