公开(公告)号：KR101566818B1

公开(公告)日：2015-11-09

申请号：KR1020080117680

申请日：2008-11-25

Applicant: (주)아모레퍼시픽 ,  고려대학교 산학협력단

Inventor： 최현 ,  여현주 ,  노민수 ,  신화영 ,  김한곤 ,  도성재 ,  김원년

IPC: A61K31/195 ,  C12Q1/68 ,  A61K31/352

Abstract: 본발명은각질형성세포에황사를처리하여발현량이유의적으로증가한물질을스크리닝함으로써황사에의한피부자극을유도하는데관여하는물질을스크리닝하는방법, 각질형성세포내에서 AhR 또는 CYP 단백질의발현량을증가시켜황사에의한피부자극을유도하는방법, 포유류에서 AhR 또는 CYP의발현을억제시킴으로써황사로인한피부자극을완화시키는방법, 상기황사에의한피부자극인자의발현정도를측정함으로써황사에의한피부자극을완화시키는물질을스크리닝하는방법, 및상기방법으로스크리닝된물질, 즉황사에의한피부자극을완화시키는물질을유효성분으로함유하는피부외용제조성물을제공한다.

公开(公告)号：KR1020100059042A

公开(公告)日：2010-06-04

申请号：KR1020080117680

申请日：2008-11-25

Applicant: (주)아모레퍼시픽 ,  고려대학교 산학협력단

Inventor： 최현 ,  여현주 ,  노민수 ,  신화영 ,  김한곤 ,  도성재 ,  김원년

IPC: A61K31/195 ,  C12Q1/68 ,  A61K31/352

CPC classification number: A61K31/195 ,  A61K31/352 ,  C12Q1/6844 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/15 ,  G01N33/5023

Abstract: PURPOSE: A method for screening a material which induces skin irritation by yellow dust is provided to reduce skin irritative factor expression and to reduce skin irritation by yellow dust. CONSTITUTION: A method for screening a material which induces skin irritation by yellow dust comprises: a step of treating yellow dust to keratinocytes; and a step of screening a gene of which expression level is significantly changed comparing with the expression of normal keratinocytes through RT-PCR. The material which induces skin irritation by yellow dust is AhR or CYP. A method for screening a material which relieves skin irritation by yellow dust comprises: a step of treating yellow dust to test cells to induce overexpression of an irritative factor; a step of treating a candidate material to the overexpressed cells; a step of measuring expression level of the irritative factor by RT-PCR. The material which relieves skin irritation by yellow dust is Trichosanthis semen. An external use skin composition for relieving skin irritation contains 0.001-1 weight% of Trichosanthis semen.

Abstract translation: 目的：提供一种筛选黄色粉尘引起皮肤刺激的材料的方法，以减少皮肤刺激因子的表达，并减少黄色粉尘的皮肤刺激。 构成：用于筛选黄色粉尘引起皮肤刺激的材料的方法包括：将黄色粉末处理成角质形成细胞的步骤; 与通过RT-PCR的正常角质化细胞的表达相比，筛选其表达水平显着变化的基因的步骤。 黄色粉尘引起皮肤刺激的物质是AhR或CYP。 用于筛选减轻黄色粉尘对皮肤刺激的材料的方法包括：处理黄色粉尘以测试细胞以诱导刺激性因子的过度表达的步骤; 将候选材料处理到过表达细胞的步骤; 通过RT-PCR测定刺激因子的表达水平的步骤。 减轻黄色粉尘的皮肤刺激性的物质是Trichosanthis精液。 用于缓解皮肤刺激的外用皮肤组合物含有0.001-1重量％的Trichosanthis精液。