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公开(公告)号：WO2014157825A1
公开(公告)日：2014-10-02
申请号：PCT/KR2014/001097
申请日：2014-02-11
Applicant: (주)진스랩
CPC classification number: C12Q1/6881 , C12Q2600/156
Abstract: 본 발명은 실시간 멀티플렉스 중합효소연쇄반응을 이용하여 HLA-A, B, DR 대립유전자를 동시에 검사할 수 있는 방법 및 키트에 관한 것으로 시료에서 추출한 DNA를 대상으로 HLA 대립유전자에 특이적으로 결합할 수 있는 시발체들과 이들 시발체에 의하여 증폭된 증폭산물을 탐지할 수 있는 형광탐침자들을 이용하여 나타나는 형광값을 가지고 HLA 대립유전자의 형별을 구분하는 방법 및 HLA 대립유전자의 형별을 구분하기 위한 키트에 관한 것이다.
Abstract translation: 本发明涉及一种能够使用实时多重聚合酶链反应同时检测HLA-A，-B和-DR等位基因的方法和试剂盒，更具体地说，涉及一种用于HLA等位基因分型的方法和试剂盒 通过使用能够特异性结合从HLA样品中提取的DNA的HLA等位基因的引物和能够检测由引物扩增的扩增产物的荧光探针所示的荧光值。
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公开(公告)号：KR101320622B1
公开(公告)日：2013-10-23
申请号：KR1020130033330
申请日：2013-03-28
Applicant: (주)진스랩
CPC classification number: C12Q1/6844 , C12N15/11 , C12Q1/686 , C12Q1/6881 , C12Q2561/113 , G01N33/582
Abstract: PURPOSE: A selection kit for HLA allele is provided to distinguish HLA allele through a real-time polymerase chain reaction without electrophoresis by using flurogenic probe and amplifying the gene in multiplex. CONSTITUTION: A selection kit for HLA allele comprises the following: a primer set capable of selective amplification by real-time PCR about the HLA allele of more than two genes selected from a group comprising HLA-A and HLA-B; and a probe set capable of probing the product amplified by the real-time PCR. A selecting method of HLA allele comprises the following steps: A real-time polymerase chain reaction (PCR) by using the kit to the refined DNA; and a selection of HLA allele by reading the result of PCR.
Abstract translation: 目的：提供HLA等位基因选择试剂盒，通过实时聚合酶链反应来区分HLA等位基因，无需使用荧光探针进行电泳，并通过多重扩增扩增基因。 构成：用于HLA等位基因的选择试剂盒包括以下：能够通过实时PCR选择性地从HLA-A和HLA-B组中选出的两种以上基因的HLA等位基因选择性扩增的引物组; 以及能够探测通过实时PCR扩增的产物的探针组。 HLA等位基因的选择方法包括以下步骤：通过使用试剂盒对精制DNA进行实时聚合酶链反应（PCR）; 并通过读取PCR结果选择HLA等位基因。
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3.发明公开
公开(公告)号：KR1020120122302A
公开(公告)日：2012-11-07
申请号：KR1020110040373
申请日：2011-04-28
Applicant: (주)진스랩
CPC classification number: C12Q1/6883 , C12N15/1003 , C12N15/117 , C12Q1/6827 , C12Q1/6858 , C12Q2521/513
Abstract: PURPOSE: A method and a kit for diagnosing fragile-x syndrome are provided to effectively and cheaply determine CGG repeat sequence methylation. CONSTITUTION: A method for diagnosing fragile-x syndrome comprises: a step of purifying genomic DNA from blood; and a step of identifying 30 or less of CGG repeat sequences and (CGG)n with 100-200 methylated CGG repeat sequence. A buffer solution for purifying genomic DNA is RBC lysis buffer, cell lysis buffer, and protein precipitation buffer. The method also comprise a step of DNA amplification for complete methylation of CGG repeat sequences using helicase and single strand binding proteins.
Abstract translation: 目的：提供用于诊断脆性-x综合征的方法和试剂盒，以有效和便宜地确定CGG重复序列甲基化。 构成：诊断脆性-x综合征的方法包括：从血液中纯化基因组DNA的步骤; 以及用100-200个甲基化CGG重复序列鉴定30个或更少个CGG重复序列和（CGG）n的步骤。 用于纯化基因组DNA的缓冲溶液是RBC裂解缓冲液，细胞裂解缓冲液和蛋白质沉淀缓冲液。 该方法还包括使用解旋酶和单链结合蛋白的CGG重复序列的完全甲基化的DNA扩增步骤。
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公开(公告)号：KR101466666B1
公开(公告)日：2014-12-01
申请号：KR1020130129854
申请日：2013-10-30
Applicant: (주)진스랩 , 가천대학교 산학협력단
Abstract: The present invention relates to a method for selectively amplifying a certain DNA fragment where a short fragment is composed of a reciprocal connection of G and C, and the front of the fragment is processed with phosphorothioate; and the short DNA fragment enables a DNA polymerase with thermal stability not to be amplified autonomously at low temperatures but to be melted at high temperatures, leading to activity recovery.
Abstract translation: 本发明涉及一种用于选择性扩增某些DNA片段的方法，其中短片段由G和C的相互连接组成，片段的前端用硫代磷酸酯加工; 并且短DNA片段使得具有热稳定性的DNA聚合酶不能在低温下自主扩增，而是在高温下熔化，导致活性恢复。
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