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公开(公告)号：KR100931863B1
公开(公告)日：2009-12-15
申请号：KR1020070089378
申请日：2007-09-04
Applicant: 가톨릭대학교 산학협력단
Abstract: 본 발명에서는 콕사키바이러스의 단백질 코딩 영역 중에서 세포내 신호 전달과 관련이 있는 특정 아미노산 서열을 암호화하는 뉴클레오티드를 변형시켜 약독화시킨 콕사키바이러스의 게놈을 이용하여 항체 또는 다른 생물학적 활성 물질을 생산할 수 있는 외부 유전자가 삽입되어 있는 발현 시스템 및 이 발현 시스템을 이용한 조성물을 제공한다. 본 발명에 따른 발현 시스템을 통하여 다양한 바이러스 질환의 백신 개발을 위한 항체 생산 및 생물학적 활성을 가진 분자생물학적인 의학적 또는 약제 조성물의 발현 시스템으로 활용될 수 있을 것으로 기대된다.
콕사키바이러스(Coxsackievirus), ITAM, 약독화(attenuation), 외래 유전자(heterogeneous gene), 벡터(vector), 발현 시스템(expression system)Abstract translation: 提供了一种使用减毒柯萨奇病毒基因组的异源基因的表达系统，用于疫苗或基因治疗的组合物。 具有柯萨奇病毒基因组的表达载体包含减毒柯萨奇病毒基因组，其中编码至少一个氨基酸的核苷酸被编码所述柯萨奇病毒的肽编码区中含有ITAM的氨基酸中的其他氨基酸的核苷酸转化。 ITAM序列存在于柯萨奇病毒的肽编码区的VP2区中。 至少一个可以揭示的异源基因被插入到减毒的柯萨奇病毒基因组中。
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公开(公告)号：KR1020090024387A
公开(公告)日：2009-03-09
申请号：KR1020070089378
申请日：2007-09-04
Applicant: 가톨릭대학교 산학협력단
Abstract: An expression system of a heterologous gene using an attenuated coxsackievirus genome is provided to be used for compositions for vaccine or gene therapy. An expression vector with a coxsackievirus genome comprises an attenuated coxsackievirus genome wherein a nucleotide coding at least one amino acid is transformed by the nucleotide coding the other amino acid among the amino acids containing ITAM in a peptide coding region of the coxsackievirus. The ITAM sequence exists in a VP2 region of a peptide coding region of the coxsackievirus. At least one heterologous gene which can be revealed is inserted in the attenuated coxsackievirus genome.
Abstract translation: 提供使用减毒的柯萨奇病毒基因组的异源基因的表达系统用于疫苗或基因治疗的组合物。 具有柯萨奇病毒基因组的表达载体包含减毒的柯萨奇病毒基因组，其中编码至少一个氨基酸的核苷酸通过在柯萨奇病毒的肽编码区中含有ITAM的氨基酸中的其它氨基酸编码的核苷酸转化。 ITAM序列存在于柯萨奇病毒的肽编码区的VP2区域中。 将至少一个可以揭示的异源基因插入减毒的柯萨奇病毒基因组中。
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3.发明授权백신으로 이용 가능하도록 변형된 콕사키바이러스의 게놈,이 게놈이 삽입된 바이러스 벡터 및 바이러스 벡터를포함한 백신 조성물 有权用作灭活疫苗的改良的同种异体病毒基因组，包含病毒载体的同种异体基因组和疫苗组合物的病毒载体
公开(公告)号：KR100757739B1
公开(公告)日：2007-09-11
申请号：KR1020060094819
申请日：2006-09-28
Applicant: 가톨릭대학교 산학협력단
Abstract: A modified coxsackievirus genome used as an attenuated vaccine is provided to reduce toxicity of coxsackievirus compared to the wild type by modifying some nucleotides, and produce the amount of antibodies similar as that of the wild type, so that disease caused by coxsackievirus is prevented. A modified coxsackievirus genome used as an attenuated vaccine in which the nucleotide encoding at least one tyrosine of ITAM(Immunoreceptor Tyrosine-based Activation sequence Motif) sequence of capsid protein expressed from the coxsackievirus genome is modified by the nucleotide encoding other amino acid is provided, wherein the ITAM sequence is present in VP2 region of coxsackievirus; the nucleotide encoding tyrosine is substituted by the nucleotide encoding phenylalanine; and the modified coxsackievirus genome is derived from CVB1, CVB2, CVB3, CVB4 and CVB5 and comprises the nucleotide sequence encoding modified VP2 peptide having the amino acid sequences of SEQ ID NO:13, 15 or 17 or the nucleotide sequence of SEQ ID NO:14, 16 or 18. The recombinant viral vector contains the modified coxsackievirus genome, at least one cloning site, at least one antibiotic-resistant gene and at least one replication origin. The vaccine composition comprises the recombinant viral vector and pharmaceutically acceptable carriers.
Abstract translation: 提供用作减毒疫苗的改良柯萨奇病毒基因组，以通过修饰一些核苷酸来减少柯萨奇病毒与野生型相比的毒性，并产生与野生型相似的抗体量，从而防止由柯萨奇病毒引起的疾病。 用作减毒疫苗的修饰的柯萨奇病毒基因组，其中编码由柯萨奇病毒基因组表达的衣壳蛋白的ITAM（基于免疫受体酪氨酸的激活序列基序）的至少一个酪氨酸的核苷酸被编码其他氨基酸的核苷酸修饰， 其中所述ITAM序列存在于柯萨奇病毒的VP2区域中; 编码酪氨酸的核苷酸被编码苯丙氨酸的核苷酸取代; 并且修饰的柯萨奇病毒基因组衍生自CVB1，CVB2，CVB3，CVB4和CVB5，并且包含编码具有SEQ ID NO：13,15或17的氨基酸序列的修饰的VP2肽的核苷酸序列或SEQ ID NO： 重组病毒载体含有修饰的柯萨奇病毒基因组，至少一个克隆位点，至少一个抗生素抗性基因和至少一个复制起点。 疫苗组合物包含重组病毒载体和药学上可接受的载体。
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