公开(公告)号：KR100801960B1

公开(公告)日：2008-02-12

申请号：KR1020060111930

申请日：2006-11-14

Applicant: 건국대학교 산학협력단

Inventor： 조쌍구 ,  최용진 ,  정미영

IPC: C07K14/00

Abstract: An mRNA binding protein XaiF(xylanase activity increasing factor) is provided to increase the activity of xylanase by being expressed simultaneously with an xyn A, increase the transcription of exogenous genes when being used with a stem-loop structure where it is attached and a strong promoter, and inhibit the decomposition of transcript, thereby being usefully used for mass-producing exogenous proteins. A stem-loop structure where XaiF as an mRNA binding protein is attached consists of a sequence of SEQ ID : NO. 3 and is used for controlling the expression of a foreign gene. A cloning vector comprises the stem-loop structure. To over-express an exogenous protein, the XaiF as the mRNA binding protein and the stem-loop structure are used, wherein the mRNA binding protein combines the stem-loop structure with a strong gene promoter including a T7 promoter.

Abstract translation: 提供mRNA结合蛋白XaiF（木聚糖酶活性增加因子），以通过与xyn A同时表达来增加木聚糖酶的活性，当与其连接的茎 - 环结构一起使用时，增加外源基因的转录，并且强壮 启动子，并抑制转录物的分解，从而有效地用于批量生产外源蛋白质。 XaiF作为mRNA结合蛋白的茎环结构由序列SEQ ID NO： 3，用于控制外源基因的表达。 克隆载体包含茎 - 环结构。 为了过表达外源蛋白，使用XaiF作为mRNA结合蛋白和茎 - 环结构，其中mRNA结合蛋白将茎 - 环结构与包含T7启动子的强基因启动子结合。