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公开(公告)号：KR100827324B1
公开(公告)日：2008-05-07
申请号：KR1020060100639
申请日：2006-10-17
Applicant: 경남과학기술대학교 산학협력단 , (주)엠젠
Abstract: 본 발명은 hGM-CSF 유전자로 형질전환된 산양 체세포의 핵과 산양 유래의 탈핵된 난자가 융합된 산양의 복제 수정란 및 (1) 산양의 체세포를 hGM-CSF 유전자로 형질전환시켜 공핵 세포를 준비하는 단계; (2) 산양으로부터 난자를 회수하여 수핵 난자를 준비하는 단계; (3) 상기 수핵 난자를 탈핵하고 공핵 세포를 이식한 후 융합시켜 수정란을 제조하는 단계를 포함한 산양 복제 수정란의 제조방법에 관한 것이다.
hGM-CSF, 공핵 세포, 수핵 난자, 산양 복제 수정란-
公开(公告)号：KR1020080034556A
公开(公告)日：2008-04-22
申请号：KR1020060100639
申请日：2006-10-17
Applicant: 경남과학기술대학교 산학협력단 , (주)엠젠
Abstract: A production method of a transgenic cloned embryo having a goat somatic cell nucleus transfected with hGM-CSF(human granulocyte macrophage-colony stimulating factor) gene is provided to produce a cloned goat expressing the hGM-CSF from the mammary gland, thereby improving the production yield of hGM-CSF. A production method of a transgenic cloned goat embryo comprises the steps of: (1) transforming a somatic cell of goat with a recombinant expression vector containing an hGM-CSF gene to prepare a nucleus donor cell; (2) collecting an ovum from the goat to prepare a nucleus recipient ovum; and (3) denucleating the recipient ovum, transplanting the nucleus donor cell into the recipient ovum and fusing them to prepare the transgenic cloned embryo.
Abstract translation: 提供了具有用hGM-CSF（人粒细胞巨噬细胞集落刺激因子）基因转染的山羊体细胞核的转基因克隆胚胎的制备方法，以从乳腺产生表达hGM-CSF的克隆山羊，从而提高产量 hGM-CSF产量。 转基因克隆山羊胚胎的制备方法包括以下步骤：（1）用含有hGM-CSF基因的重组表达载体转化山羊的体细胞以制备核供体细胞; （2）从山羊收集卵，制备受精卵; （3）使受体卵脱核，将核供体细胞移植到受体卵中并使其融合以制备转基因克隆的胚胎。
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公开(公告)号：KR100679151B1
公开(公告)日：2007-02-05
申请号：KR1020050088544
申请日：2005-09-23
Applicant: 경남과학기술대학교 산학협력단
Abstract: A method for performing animal embryo biopsy using laser is provided to be able to rapidly and conveniently select and transplant only desired fertilized eggs with decreasing damage of the fertilized eggs, thereby increasing production efficiency of domestic animal. The method comprises the steps of: (a) washing fertilized eggs of a cow at the development stage such as 8-cell stage, blastocyst stage and gemmule stage with a buffer solution such as D-PBS and locating it at a buffer solution containing serum; (b) after fixing the fertilized eggs with a compensation pipette where the end portion is inclinedly worked, applying laser having a strength of 40-60 microsec 2-3 times to zone pellucida of the fertilized eggs so as to perforate the zone pellucida; and (c) after locating an enucleation pipette, where the end portion is inclinedly worked, to be opposite to the compensation pipette along the center of the fertilized eggs where the zone pellucida is perforated, applying pressure thereto using two pipettes to emit blastomere.
Abstract translation: 提供一种利用激光进行动物胚胎活组织检查的方法，能够快速方便地选择和移植所需的受精卵，同时减少受精卵的损伤，从而提高家畜的生产效率。 该方法包括以下步骤：（a）用缓冲液如D-PBS洗涤处于发育阶段如8细胞期，胚泡期和gemmule阶段的母牛的受精卵，并将其置于含有血清的缓冲液 ; （b）用补偿移液管固定受精卵，其中端部倾斜加工，将强度为40-60微秒2-3倍的激光施加到受精卵的区域透明带上，以穿透该区域的透明带; 和（c）在将端部倾斜加工的去核移液管定位在补偿移液管的沿着受精卵的中心的地方，在所述受精卵的中心穿孔区域，使用两个移液管施加压力以发射卵裂球。
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