公开(公告)号：KR1020080008802A

公开(公告)日：2008-01-24

申请号：KR1020060068539

申请日：2006-07-21

Applicant: 경상대학교산학협력단

Inventor： 정태성 ,  김영림 ,  신기욱

IPC: C07K16/18 ,  C07K16/00

CPC classification number: C07K16/42 ,  C07K1/22 ,  C07K2317/20 ,  G01N33/532 ,  G01N33/543

Abstract: An immunoglobulin isolated and purified from Sebastes schlegeli is provided to be used as a diagnosing kit including a monoclonal antibody against the immunoglobulin, thereby providing useful information for studying the humoral immunity of the Sebastes schlegeli against pathogens and developing a diagnostic marker and a vaccine. A method for preparing a monoclonal antibody against an immunoglobulin of Sebastes schlegeli comprises the steps of: (a) after immunizing the Sebastes schlegeli with an immunoglobulin of an animal, isolating serum of the Sebastes schlegeli; (b) purifying immunoglobulin from the serum using protein A, mannan binding protein(MBP), or immunoaffinity column; and (c) after collecting spleens from mouse immunized by the immunoglobulin, fusing them with myeloma cells, wherein the purified immunoglobulin consists of a heavy chain having a molecular weight of 70 kDa and a light chain having a molecular weight of 25 kDa. A kit for detecting immunoglobulin of Sebastes schlegeli comprises a solid support where a monoclonal antibody regarding the immunoglobulin of the Sebastes schlegeli is fixed by a covalent bond or a physical absorption, at least one solution including detectable color developing agent, a buffered washing solution and a sample required for quality management. To diagnose the humoral immunity of the Sebastes schlegeli, the detecting kit is used.

Abstract translation: 提供从Sebastes schlegeli分离和纯化的免疫球蛋白，用作诊断试剂盒，其包括针对免疫球蛋白的单克隆抗体，从而提供用于研究Sebastes schlegeli针对病原体的体液免疫性并开发诊断标记物和疫苗的有用信息。 制备针对Sebastes schlegeli免疫球蛋白的单克隆抗体的方法包括以下步骤：（a）用动物免疫球蛋白免疫Sebastes schlegeli，分离Sebastes schlegeli的血清; （b）使用蛋白A，甘露聚糖结合蛋白（MBP）或免疫亲和柱从血清纯化免疫球蛋白; 和（c）从用免疫球蛋白免疫的小鼠收集脾后，将其与骨髓瘤细胞融合，其中纯化的免疫球蛋白由分子量为70kDa的重链和分子量为25kDa的轻链组成。 用于检测Sebastes schlegeli免疫球蛋白的试剂盒包括固体支持物，其中关于Sebastes schlegeli免疫球蛋白的单克隆抗体通过共价键或物理吸收固定，至少一种溶液，包括可检测的显色剂，缓冲的洗涤溶液和 样品需要质量管理。 为了诊断Sebastes schlegeli的体液免疫，使用检测试剂盒。

公开(公告)号：KR1020080008799A

公开(公告)日：2008-01-24

申请号：KR1020060068534

申请日：2006-07-21

Applicant: 경상대학교산학협력단

Inventor： 정태성 ,  김영림 ,  신기욱

IPC: C07K16/18 ,  C07K16/00

CPC classification number: C07K16/18 ,  C07K1/22 ,  G01N33/532 ,  G01N33/543

Abstract: An immunoglobulin purified from Paralichthys olivaceus is provided to be used for studying about L.garvieae through further study utilized by the same and provide useful information required for developing a diagnostic marker and a vaccine. An immunoglobulin is characterized in that it is isolated and purified from Paralichthys olivaceus serum and consists of a heavy chain having an isoelectric point of 5.0-6.4 and a molecular weight of 66-68 kDa and a light chain having an isoelectric point of 4-7 and a molecular weight of 25-27 kDa. A method for preparing a monoclonal antibody against the immunoglobulin of Paralichthys olivaceus comprises the steps of: (a) after immunizing the Paralichthys olivaceus with an immunoglobulin of an animal, isolating serum of the Paralichthys olivaceus; (b) purifying immunoglobulin from the serum using protein A, mannan binding protein, or immunoaffinity column; and (c) after collecting spleens from mouse immunized by the immunoglobulin, fusing them with myeloma cells. A kit for detecting immunoglobulin of Paralichthys olivaceus comprises a solid support where a monoclonal antibody regarding the immunoglobulin of the Paralichthys olivaceus is fixed by a covalent bond or a physical absorption, at least one solution including detectable color developing agent, a buffered washing solution and a sample required for quality management.

Abstract translation: 提供从Paralichthys olivaceus纯化的免疫球蛋白，用于通过进一步研究L.garvieae进行研究，并提供开发诊断标记和疫苗所需的有用信息。 免疫球蛋白的特征在于其从Paralichthys olivaceus血清中分离和纯化，并且由等电点为5.0-6.4，分子量为66-68kDa的重链和等电点为4-7的轻链组成 分子量为25-27 kDa。 制备针对Paralichthys olivaceus免疫球蛋白的单克隆抗体的方法包括以下步骤：（a）用动物免疫球蛋白免疫Paralichthys olivaceus后，分离橄榄属Paralichthys olivaceus的血清; （b）使用蛋白A，甘露聚糖结合蛋白或免疫亲和柱从血清纯化免疫球蛋白; 和（c）收集由免疫球蛋白免疫的小鼠的脾脏，将其与骨髓瘤细胞融合。 用于检测枸杞子的免疫球蛋白的试剂盒包括固体支持物，其中关于通过共价键或物理吸收固定缬氨酸Paralichthys的免疫球蛋白的单克隆抗体，至少一种溶液，包括可检测的显色剂，缓冲的洗涤溶液和 样品需要质量管理。