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公开(公告)号：KR100930973B1
公开(公告)日：2009-12-10
申请号：KR1020070097352
申请日：2007-09-27
Applicant: 고려대학교 산학협력단
Inventor： 구만복 , 콜칼모하메드제이브드후세인니아지 , 김연석 , 김윤진 , 이수진
IPC: C12Q1/68
CPC classification number: C12Q1/6876
Abstract: 본 발명은 옥시테트라사이클린에 특이적으로 결합하는 DNA 앱타머에 관한 것으로, 더욱 구체적으로 랜덤 DNA 라이브러리로부터 선택된 옥시테트라사이클린에 특이적으로 결합하는 DNA 앱타머 및 그 제조방법에 관한 것이다.
본 발명에 따르면, 랜덤 DNA pool로부터 항생물의 한 종류인 옥시테트라사이클린에 대해 특이적으로 높은 친화도를 보이는 DNA 앱타머를 SELEX process의 변형된 방법인 FluMag-SELEX process를 이용하여 선별하고, 선별된 DNA 앱타머들의 염기서열, 특성 및 옥시테트라사이클린에 대한 결합력을 분석하였다. 따라서 본 발명의 DNA 앱타머를 이용하여 상하수원, 식뭄, 인체 등으로부터 잔류 항생물질의 검출을 보다 효과적 수행할 수 있다.
옥시테트라사이클린, 항생물질, SELEX, DNA 앱타머, 생물농축Abstract translation: 本发明涉及的是，更具体地，应用DNA特异性结合从随机DNA文库中选择驯氧基四环素，和特异性结合到氧 - 四环素的DNA适体的制造方法。
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Patent Agency Ranking
公开(公告)号：KR100930974B1
公开(公告)日：2009-12-10
申请号：KR1020070097366
申请日：2007-09-27
Applicant: 고려대학교 산학협력단
IPC: C12Q1/68
Abstract: 본 발명은 RBP4(Retinol Binding Protein 4)에 특이적으로 결합하는 DNA 앱타머에 관한 것으로, 더욱 구체적으로 랜덤 DNA 라이브러리로부터 선택된 RBP4에 특이적으로 결합하는 DNA 앱타머 및 그 제조방법에 관한 것이다.
본 발명에 따르면, 랜덤 DNA pool로부터 제 2형 당뇨병의 바이오마커인 RBP4에 대해 특이적으로 높은 친화도를 보이는 DNA 앱타머를 SELEX process의 변형된 방법인 FluMag-SELEX process를 이용하여 선별하고, 선별된 DNA 앱타머들의 염기서열, 특성 및 RBP4에 대한 결합력을 분석하였다. 따라서 본 발명의 DNA 앱타머를 이용하여 제 2형 당뇨병을 보다 효과적으로 진단할 수 있다.
RBP4, DNA 앱타머, 제 2형 당뇨병, 바이오마커, 친화도, SELEXAbstract translation: 本发明涉及涉及结合到RBP4特定DNA适体（视黄醇结合蛋白4），更具体地，应用DNA特异性结合从随机DNA文库中选择驯RBP4及其制造的方法。
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公开(公告)号：KR1020090032276A
公开(公告)日：2009-04-01
申请号：KR1020070097352
申请日：2007-09-27
Applicant: 고려대학교 산학협력단
Inventor： 구만복 , 콜칼모하메드제이브드후세인니아지 , 김연석 , 김윤진 , 이수진
IPC: C12Q1/68
CPC classification number: C12Q1/6876 , C12N15/115 , C12Q1/686
Abstract: Provided is a DNA aptamer, which is specifically bound to oxytetracycline selected from random DNA library in order to detect residue antibiotic from foods, human bodies, or water supply and drainage facilities. A DNA aptamer has one base sequence among SEQ ID NO:1 to 15. A method for preparing the DNA aptamer comprises the following steps of: reacting the oxytetracycline and magnetic beads to a borate buffer solution to induce covalent bonds; mixing the oxytetracycline-magnetic beads to a DNA pool having 30-50 numbers of bases in a center and a primer region for PCR(polymerase chain reaction) in an end so as to induce bonding at a room temperature; and separating DNA from the oxytetracycline-magnetic beads using a magnet.
Abstract translation: 提供了一种DNA适体，其特异性结合于选自随机DNA文库的土霉素，以便从食物，人体或给排水设施中检测残留抗生素。 DNA适配体在SEQ ID NO：1〜15中具有一个碱基序列。一种制备DNA适体的方法包括以下步骤：将土霉素和磁珠与硼酸盐缓冲溶液反应以诱导共价键; 将土霉素 - 磁珠与中心的30-50个碱基的DNA库和末端的PCR引物区域（聚合酶链式反应）混合，以在室温下诱导结合; 并使用磁铁从土霉素 - 磁珠分离DNA。
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公开(公告)号：KR100801983B1
公开(公告)日：2008-02-12
申请号：KR1020070038282
申请日：2007-04-19
Applicant: 고려대학교 산학협력단
CPC classification number: C02F3/34 , C02F1/28 , C02F1/66 , C02F2303/02
Abstract: A liposome-aptamer complex is provided to remove endocrine disrupting chemicals effectively by positioning the hexane aptamer in a submicron-sized liposome consisted of a bilayer lipid membrane so that a hexane aptamer peculiarly bonded to endocrine disrupting chemicals can be used in an actual underwater environment, thereby bonding well the hexane aptamer to the endocrine disrupting chemicals, and a method for removing endocrine disrupting chemicals in an underwater environment by using the liposome-aptamer complex is provided. A liposome-aptamer complex for removing endocrine disrupting chemicals is characterized in that a hexane aptamer peculiarly bondable with endocrine disrupting chemicals is introduced into a liposome consisted of a bilayer lipid membrane. A preparation method of a liposome-aptamer complex for removing endocrine disrupting chemicals comprises the steps of: developing an aptamer perculiar to target endocrine disrupting chemicals by an SELEX(Systematic Evolution of Ligands by Exponential enrichment) method; dissolving a single lipid material into chloroform, evaporating the dissolved solution to form a dilayer lipid membrane, injecting a buffer capable of being bonded optimally to the endocrine disrupting chemicals into the dilayer lipid membrane, and forming a liposome from the mixture using a sonicator; mixing the aptamer and the liposome in a tris bubber; mixing an ethanol/calcium chloride solution with the mixed solution, and dialyzing the mixed solution with a PBS solution; and purifying a liposome-aptamer comple using a high speed centrifuge.
Abstract translation: 提供脂质体 - 适体复合物以通过将己烷适体定位在由双层脂质膜组成的亚微米尺寸脂质体中以有效地去除内分泌干扰化学物质，使得在实际的水下环境中可以使用特异性结合内分泌干扰物质的己烷适体， 从而使己烷适体与内分泌干扰物质结合良好，并且提供了通过使用脂质体 - 适体复合物在水下环境中去除内分泌干扰物质的方法。 用于去除内分泌干扰物质的脂质体 - 适体复合物的特征在于将由内分泌干扰物质特异性结合的己烷适体引入由双层脂质膜组成的脂质体中。 用于去除内分泌干扰物质的脂质体 - 适体复合物的制备方法包括以下步骤：通过SELEX（通过指数富集的配体系统演化）方法开发靶向内分泌干扰化学物质的适体; 将单一脂质材料溶解在氯仿中，蒸发溶解的溶液以形成二层脂质膜，将能够最佳地结合到内分泌干扰物质的缓冲液注入到二层脂质膜中，并使用超声波仪从该混合物形成脂质体; 将适体和脂质体混合在三泡液中; 将乙醇/氯化钙溶液与混合溶液混合，并用PBS溶液透析混合溶液; 并使用高速离心机纯化脂质体 - 适体。
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公开(公告)号：KR100828481B1
公开(公告)日：2008-05-13
申请号：KR1020060133082
申请日：2006-12-22
Applicant: 고려대학교 산학협력단
IPC: C12Q1/68
CPC classification number: G01N33/5308 , C12N15/115 , C12Q1/6834 , C12Q2563/131
Abstract: A method for removing endocrine disrupting chemicals is provided to improve selectivity of toxic materials endocrine disrupting chemicals by using a DNA aptamer specifically binding to the endocrine disrupting chemicals, instead of multi-process adsorbent or nano-filter. A method for removing endocrine disrupting chemicals comprises the steps of: selecting a DNA aptamer specifically binding to the endocrine disrupting chemicals, having the nucleotide sequence of SEQ ID NO:1 from the random ssDNA(single-stranded DNA) library by using SELEX(systematic evolution of ligands by exponential enrichment) process; modifying one end of the selected DNA aptamer with biotin for fixing to the surface of beads; fixing the modified DNA aptamer to the bead surface; and inducing the binding between the fixed DNA aptamer and the endocrine disrupting chemicals, wherein the endocrine disrupting chemicals include 17beta-estradiol, bisphenol A, nonyl phenol and PHB(poly-hydroxybutyrate). Further, the beads are coated with a streptavidin.
Abstract translation: 提供了一种去除内分泌干扰物质的方法，通过使用特异性结合内分泌干扰物质的DNA适配体代替多工艺吸附剂或纳米过滤器来提高毒性物质内分泌干扰物质的选择性。 一种去除内分泌干扰物质的方法包括以下步骤：通过使用SELEX（系统的）从随机ssDNA（单链DNA）文库中选择具有与内分泌干扰物质特异性结合的DNA适体，具有SEQ ID NO：1的核苷酸序列 配体进化指数富集）过程; 用生物素修饰选定的DNA适体的一端，固定在珠的表面上; 将修饰的DNA适配体固定到珠粒表面; 诱导固定DNA适体和内分泌干扰物质之间的结合，其中内分泌干扰物质包括17β-雌二醇，双酚A，壬基苯酚和PHB（聚羟基丁酸酯）。 此外，珠子用链霉亲和素包被。
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公开(公告)号：KR1020090032285A
公开(公告)日：2009-04-01
申请号：KR1020070097366
申请日：2007-09-27
Applicant: 고려대학교 산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/6876 , C12N15/115 , C12Q1/686
Abstract: A DNA aptamer, which is specifically bound to a rentinol-binding protein(RBP4), is provided to effectively diagnose the type 2 diabetes. A DNA aptamer which can be specifically combined with a rentinol-binding protein(RBP4) has one base sequence among SEQ ID NO:1 to 10. A composition for diagnosing the type 2 diabetes comprises the DNA aptamer specifically combined with a rentinol-binding protein. The composition for diagnosing the type 2 diabetes measures a level of blood RBP4. The RBP4 indicates a biomarker of the type 2 diabetes. A method for preparing the DNA aptamer comprises the following steps of: reacting the RBP4 and magnetic beads to a borate buffer solution to induce covalent bonds; mixing the RBP4-magnetic beads to a DNA pool having 30-50 numbers of bases in a center and a primer region for PCR(polymerase chain reaction) in an end so as to induce bonding at a room temperature.
Abstract translation: 提供特异性结合于蛋白质结合蛋白（RBP4）的DNA适体，以有效诊断2型糖尿病。 可以特异性结合二醇结合蛋白（RBP4）的DNA适配体在SEQ ID NO：1〜10中具有一个碱基序列。用于诊断2型糖尿病的组合物包括特异性结合了含有二醇结合蛋白的DNA适体 。 用于诊断2型糖尿病的组合物测量血液RBP4水平。 RBP4表示2型糖尿病的生物标志物。 制备DNA适体的方法包括以下步骤：将RBP4和磁珠与硼酸盐缓冲溶液反应以诱导共价键; 将RBP4-磁珠混合到具有中心的30-50个碱基的DNA库和用于PCR的聚合酶链反应的引物区域（聚合酶链式反应），以在室温下诱导结合。
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