公开(公告)号：KR1020070002279A

公开(公告)日：2007-01-05

申请号：KR1020050057720

申请日：2005-06-30

Applicant: 고려대학교 산학협력단

Inventor： 신동훈 ,  조홍연 ,  홍범식 ,  김혜경 ,  김미정 ,  최수정 ,  서영민 ,  허호진

IPC: A61K36/488 ,  A61P25/28

CPC classification number: A61K36/488 ,  A23V2200/322 ,  A61K2236/33

Abstract: The extract of Pueraria thunbergiana or a low molecular weight flavonoid extracted therefrom is provided to activate choline acetyltransferase in brain nerve cells, so that it is useful for preventing or treating brain nerve disease. A composition for activating choline acetyltransferase in brain nerve cells comprises the extract of Pueraria thunbergiana as an effective ingredient, wherein the extract of Pueraria thunbergiana is prepared with methanol or ethanol. The low molecular weight flavonoid extracted from the extract of Pueraria thunbergiana is 4',7-dihydroxyisoflavone(daidzein), wherein low molecular weight flavonoid is purified by (i) extracting dried Pueraria thunbergiana with methanol or ethanol, (ii) fractionating the methanol or ethanol extract with hexane, chloroform and ethylacetate sequentially, (iii) subjecting the ethylacetate fraction to first and second open silica gel column, (iv) subjecting the active fraction to thin layer chromatography, and (v) subjecting the active fraction to high pressure liquid chromatography.

Abstract translation: 提供葛根提取物或从其中提取的低分子量类黄酮，以激活脑神经细胞中的胆碱乙酰转移酶，从而可用于预防或治疗脑神经疾病。 用于激活脑神经细胞中胆碱乙酰转移酶的组合物包含葛根葛根提取物作为有效成分，其中葛根提取物用甲醇或乙醇制备。 从葛根提取物中提取的低分子量类黄酮为4'，7-二羟基异黄酮（黄豆苷原），其中低分子量类黄酮通过（i）用甲醇或乙醇提取干燥的葛根草提纯，（ii）分馏甲醇或 乙醇提取液依次用己烷，氯仿和乙酸乙酯萃取，（iii）使乙酸乙酯部分进行第一和第二开放硅胶柱，（iv）使活性部分进行薄层色谱，和（v）使活性部分经受高压液体 色谱法。