公开(公告)号：KR1020070117294A

公开(公告)日：2007-12-12

申请号：KR1020060051371

申请日：2006-06-08

Applicant: 고려대학교 산학협력단

Inventor： 황병국 ,  안수현 ,  손기훈

IPC: C12N15/29 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/11 ,  C12N15/1017 ,  C12N15/1086 ,  C12N15/63 ,  C12Q1/6876 ,  C12Q1/689

Abstract: A red pepper gene CaPMEI1 encoding a pectin methylesterase inhibitor protein is provided to screen resistance against plant disease and environmental stress rapidly, so that the gene is useful for development of transgenic plants having enhanced resistance against plant disease and environmental stress. A red pepper gene CaPMEI1 encoding a pectin methylesterase inhibitor protein having the amino acid sequence of SEQ ID NO:2 is isolated from Capsicum annuum L. cv. Hanbyul and has the nucleotide sequence of SEQ ID NO:1. A recombinant vector pET32a::CaPMEI1 or pBIN35S::CaPMEI1::GFP contains the red pepper gene CaPMEI1. A method for screening the resistance against plant disease and environmental stress comprises the steps of: isolating RNA from a red pepper plant treated by pathogenic bacterium, chemical or environment stress; subjecting the isolated RNA to electrophoresis and transferring into nylon film(Hybond N+); reacting the nylon film with the CaPMEI1 gene probe labeled with 32P; and confirming discriminative expression of the CaPMEI1 gene.

Abstract translation: 提供了编码果胶甲酯酶抑制蛋白的红辣椒基因CaPMEI1，以快速筛选植物病害和环境胁迫的抗性，使得该基因对于具有增强的抗植物病害和环境胁迫抗性的转基因植物的开发是有用的。 编码具有SEQ ID NO：2的氨基酸序列的果胶甲酯酶抑制剂蛋白质的红辣椒基因CaPMEI1从辣椒（Capsicum annuum L.cv。 并具有SEQ ID NO：1的核苷酸序列。 重组载体pET32a :: CaPMEI1或pBIN35S :: CaPMEI1 :: GFP含有红辣椒基因CaPMEI1。 筛选对植物病害和环境胁迫的抗性的方法包括以下步骤：从病原菌，化学或环境胁迫处理的红辣椒植物中分离RNA; 将分离的RNA进行电泳并转移到尼龙膜（Hybond N +）中; 使尼龙膜与标记有32P的CaPMEI1基因探针反应; 并证实CaPMEI1基因的鉴别表达。

公开(公告)号：KR100794469B1

公开(公告)日：2008-01-17

申请号：KR1020060051371

申请日：2006-06-08

Applicant: 고려대학교 산학협력단

Inventor： 황병국 ,  안수현 ,  손기훈

IPC: C12N15/29 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/68

Abstract: 본 발명은 고추식물의 세균병인 고추 세균성 점무늬병균 (
Xanthomonas
campestris pv.
vesicatoria )의 병원성균과 비병원성균에 감염되었을 경우 특이적으로 유도되는 신규 저항성 유전자인 펙틴 메칠에스터라아제 억제단백질을 코딩하는 CaPMEI1 유전자, 상기 유전자를 이용한 병저항성 및 환경스트레스 저항성 탐색방법 및 저항성 형질전환 식물체 제작에 관한 것이다. 상기한 본 발명에 의하면, 식물체 병원균의 방어반응의 표지 및 환경스트레스 내성 식물의 분자육종을 위한 유전재료를 제공할 수 있고, 식물병 저항성 등의 탐색을 촉진시킬 수 있다.
고추, 식물병, 저항성, 세균성 점무늬병, 펙틴 메칠에스터라아제

公开(公告)号：KR100796164B1

公开(公告)日：2008-01-21

申请号：KR1020060106749

申请日：2006-10-31

Applicant: 고려대학교 산학협력단

Inventor： 황병국 ,  안수현

IPC: C12N15/29 ,  C07K14/415

Abstract: A plant membrane receptor protein gene CaMRP1 derived from pepper is provided to confer resistance against environmental stresses including methyl jasmonate and high salt on plants by overexpressing the gene, so that the gene is useful for producing and screening transgenic plants having environmental stress resistance. A plant membrane receptor protein gene CaMRP1 derived from pepper(Capsicum annuum L. cv. Hanbyul) has the nucleotide sequence of SEQ ID NO:1, wherein the plant membrane receptor protein has the amino acid sequence of SEQ ID NO:2. An environmental stress resistant transgenic plant is produced by inserting the plant membrane receptor protein gene CaMRP1 into pBIN35S to prepare a recombinant vector, and transforming a plant such as Arabidopsis thaliana with the recombinant vector.

Abstract translation: 提供来源于胡椒的植物膜受体蛋白基因CaMRP1以通过过表达该基因来赋予植物上的茉莉酸甲酯和高盐对环境胁迫的抗性，使得该基因可用于产生和筛选具有环境胁迫性的转基因植物。 源自辣椒（Capsicum annuum L.cv.Hanbyul）的植物膜受体蛋白基因CaMRP1具有SEQ ID NO：1的核苷酸序列，其中植物膜受体蛋白具有SEQ ID NO：2的氨基酸序列。 通过将植物膜受体蛋白基因CaMRP1插入pBIN35S中制备重组载体，并用重组载体转化拟南芥属植物，从而产生环境胁迫转基因植物。