公开(公告)号：KR101106061B1

公开(公告)日：2012-01-18

申请号：KR1020090049985

申请日：2009-06-05

Applicant: 고려대학교 산학협력단

Inventor： 한성옥 ,  전유진 ,  현정은

IPC: C12N15/81 ,  C12N15/52 ,  C12P7/06 ,  C12R1/145

CPC classification number: Y02E50/17

Abstract: 본 발명은 클로스트리디움 속 (
Clostridium sp.) 미생물 유래의 엔도-베타-1,4-글루칸아제-이 (endo-β-1,4-glucanaseE, 1,4-β-D-glucan glucanohydrolase, carboxymethylcellulase, CMCase; EC 3.2.1.4) 와 사카로마이콥시스 속(
Saccharomycopsis sp.) 미생물 유래의 베타-글루코시다제 (β-glucosidase; EC 3.2.1.21) 유전자가 도입된 재조합 벡터 및 그 형질 전환체에 관한 것이다.
베타-글루코시다아제, 재조합 벡터, 형질전환체

公开(公告)号：KR1020100131208A

公开(公告)日：2010-12-15

申请号：KR1020090049985

申请日：2009-06-05

Applicant: 고려대학교 산학협력단

Inventor： 한성옥 ,  전유진 ,  현정은

IPC: C12N15/81 ,  C12N15/52 ,  C12P7/06 ,  C12R1/145

CPC classification number: Y02E50/17

Abstract: PURPOSE: A yeast transformant with improved bio-ethanol productivity is provided to use in SSF(simulateous saccharification and fermentation) or consolidated bioprocessing. CONSTITUTION: A recombinant vector, p-alpha-EgE-alpha-BG1 comprises a gene encoding Clostridium sp-derived endoglucanase and gene encoding Saccharomycopsis sp.-derived betaglucosidase. A transformant transformed by the recombinant vector, p-alpha-EgE-alpha-BG1. A bio-ethanol is produced by culturing transformed yeast under the presence of substrate. The substrate is starch or fiber.

Abstract translation: 目的：提供具有改善生物乙醇生产力的酵母转化体，用于SSF（模拟糖化和发酵）或固结生物处理。 构成：重组载体p-α-EgE-α-BG1包含编码梭菌属sp-衍生内切葡聚糖酶的基因和编码Saccharomycopsis sp.衍生的β-葡糖苷酶的基因。 由重组载体p-α-EgE-α-BG1转化的转化体。 通过在底物存在下培养转化的酵母生产生物乙醇。 底物是淀粉或纤维。

公开(公告)号：KR100969328B1

公开(公告)日：2010-07-09

申请号：KR1020090061643

申请日：2009-07-07

Applicant: (주) 나노톡스텍 ,  고려대학교 산학협력단

Inventor： 한성옥 ,  전유진

IPC: C12N15/63 ,  C12N15/56 ,  C12N1/21 ,  C12R1/145

Abstract: PURPOSE: A method for preparing yeast transformant with improved bioethanol productivity by introducing Clostridium-derived endoglucanase gene to yeast. CONSTITUTION: A recombinant vector contains a nucleic acid sequence encoding endo-beta-1,4-glucanase of sequence number 1 which is derived from Clostridium cellulovorans and a nucleic acid encoding beta-glucosidae of sequence number 2 which is derived from Saccharomycopsis fibuligera. A transformant(deposit number: KCCM11005P) is prepared by transforming Saccharomyces cerevisiae with the recombinant vector. The transformant is used for producing bioethanol by culture with biomass. The biomass is a cellulose biomass.

Abstract translation: 目的：通过将酵母菌来源的内切葡聚糖酶基因引入酵母转化体，提高生物乙醇生产力的方法。 构成：重组载体含有编码序列号1的内切-β-1,4-葡聚糖酶的核酸序列，其衍生自Clostridium cellulovorans和编码序列号2的β-葡糖苷酶的核酸，所述核酸编码源自糖酵母菌（Saccharomycopsis fibuligera）。 通过用重组载体转化酿酒酵母制备转化体（保藏号：KCCM11005P）。 转化体用于通过生物质培养生产生物乙醇。 生物质是一种纤维素生物质。