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公开(公告)号：KR1020120117190A
公开(公告)日：2012-10-24
申请号：KR1020110034791
申请日：2011-04-14
Applicant: 고려대학교 산학협력단
CPC classification number: C12Q1/02 , G01N33/5008 , G01N33/532 , G01N33/56966 , G01N2333/705
Abstract: PURPOSE: A bio-sensing method using animal cells and a bio kit for infection diagnosis using the same are provided to monitor the source of infection in real time. CONSTITUTION: A bio-sensing method using animal cells comprises the following steps: inoculating source of infection in a culture medium in which the animal cells are cultured; and reacting probes(15) in which marker is attached with biomarkers(13) of the animal cells. The source of infection is alive pathogenic microorganism. The animal cell is in cell starvation state. The animal cells(14) are adhesive type cell or stray type cell. The biomarker is cell receptor protein, cell attached protein, glycolipid, or glycoprotein. The cell adhesive protein is integrin, selectin or cadherin. The probe is antibody which specifically binds with the bio markers, binding protein, nucleic acid, enzyme, aptamer or peptide. A bio kit for infection diagnosis using the animal cells comprises wells which contain the animal cells and probes which detect bio markers of the animal cells.
Abstract translation: 目的：提供使用动物细胞的生物感测方法和使用其的感染诊断的生物试剂盒来实时监测感染源。 构成：使用动物细胞的生物感测方法包括以下步骤：在培养动物细胞的培养基中接种感染源; 和将标记物与动物细胞的生物标志物（13）连接的探针（15）。 感染源是活的致病微生物。 动物细胞处于细胞饥饿状态。 动物细胞（14）是粘附型细胞或杂散型细胞。 生物标志物是细胞受体蛋白，细胞附着蛋白，糖脂或糖蛋白。 细胞粘附蛋白是整联蛋白，选择蛋白或钙粘蛋白。 探针是与生物标记，结合蛋白，核酸，酶，适体或肽特异性结合的抗体。 用于使用动物细胞进行感染诊断的生物试剂盒包括含有检测动物细胞的生物标志物的动物细胞和探针的孔。
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公开(公告)号：KR1020130093050A
公开(公告)日：2013-08-21
申请号：KR1020130076543
申请日：2013-07-01
Applicant: 고려대학교 산학협력단
CPC classification number: C12Q1/02 , G01N33/5005
Abstract: PURPOSE: A biosensing method is provided to simply analyze source of infection, and to simplify the process by comprising a stage of injecting the resource of infection to a culture medium in which animal cell is cultivated, and reacting a biomarker of the animal cell with a probe attached with a marker. CONSTITUTION: A biosensing method using animal cell (14) comprises the steps of: expressing toll-similar acceptor by injecting and culturing injection resource of separated and concentrated Escherichia coli or Shigella sonnei into a culture medium in which at least one animal cell is cultured among lung cell, endocervical cell, and macrophage; and reacting the expressed toll-similar acceptor with a probe (15) adhered with a marker. In the first stage, if the pathogenic microorganism is Escherichia coli, the animal cell is the lung cell, and the toll-similar acceptor is toll-similar acceptor 1, if the pathogenic microorganism is Shigella sonnei, the animal cell is the endocervical cell or macrophage, and the toll-similar acceptor is toll-similar acceptor 2.
Abstract translation: 目的：提供一种简单分析感染源的生物传感方法，并通过将感染资源注入培养动物细胞的培养基中并使动物细胞的生物标志物与 探头附有标记。 构成：使用动物细胞的生物传感方法（14）包括以下步骤：通过将分离浓缩的大肠杆菌或志贺氏菌志贺氏菌的注射资源注射培养至培养至少一种动物细胞的培养基中来表达同样的受体 肺细胞，子宫颈癌细胞和巨噬细胞; 并使所述表达的长期相似受体与粘附有标记的探针（15）反应。 在第一阶段，如果致病微生物是大肠杆菌，则动物细胞是肺细胞，并且同样的受体是同样的受体1，如果病原微生物是志贺氏菌（Shigella sonnei），则动物细胞是子宫颈细胞或 巨噬细胞，长途相似的受体是长途相似的受体2。
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公开(公告)号：KR101503830B1
公开(公告)日：2015-03-18
申请号：KR1020110034791
申请日：2011-04-14
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 동물 세포를 이용한 바이오센싱 방법 및 동물 세포를 이용한 감염 진단 바이오 키트에 관한 것으로서, 보다 상세하게는 감염 및 오염 등에 의한 외부환경의 변화 시, 동물 세포 표면에 발현되어 감염원과 반응하는 세포 수용체, 당단백, 당지질 등의 바이오 마커를 탐지체와 결합시킴으로써, 신속하고 간편하게 감염원에 대한 감지를 실시간으로 모니터링 할 수 있는 동물 세포를 이용한 바이오센싱 방법 및 동물 세포를 이용한 감염 진단 바이오 키트에 관한 것이다.
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