公开(公告)号：KR1020150126187A

公开(公告)日：2015-11-11

申请号：KR1020140053479

申请日：2014-05-02

Applicant: 대구가톨릭대학교산학협력단

Inventor： 김태완 ,  구본철 ,  권모선

IPC: C12N15/867 ,  C12N5/10 ,  A01K67/027

CPC classification number: C12N15/867

Abstract: 본발명은목적유전자를동물의뇌하수체세포에서특이적으로발현시키는레트로바이러스발현벡터에관한것으로, 보다상세하게는서열번호 1의쥐의성장호르몬프로모터유전자를함유하여, downstream 영역의목적유전자를뇌하수체세포에서특이적으로발현시키는것을특징으로하는레트로바이러스발현벡터에관한것이다.

Abstract translation: 本发明涉及特异性表达动物特异性靶标的逆转录病毒表达载体，更具体地，涉及含有由SEQ ID NO：1表示的大鼠生长激素基因，因此特异性表达靶基因的逆转录病毒表达载体 在pituicytes的下游区域。

公开(公告)号：KR101470595B1

公开(公告)日：2014-12-10

申请号：KR1020120084352

申请日：2012-08-01

Applicant: 대구가톨릭대학교산학협력단

Inventor： 김태완 ,  구본철 ,  권모선

IPC: C07K19/00 ,  C12N15/62 ,  C12N15/867

Abstract: 본발명은 GFP의특성을갖고있어 480nm 파장의광조사하에서고유의형광을방출하며발현시 세포막을통과하여외부로분비되므로,유전자를리포터유전자로사용하면살아있는세포, 조직또는동물에서장파장의자외선을조사하여간단하게그 발현을관찰할수 있는것을특징으로하는 GFP(green fluorescent protein) 또는그 변형단백질과래트의 FSHB 신호단백질의재조합단백질, 이를코딩하는유전자및 이유전자의발현을위한벡터에관한것이다. 본발명에의하면세포를파쇄하지않고세포가살아있는상태에서발현량을정량할수 있기때문에연속적인(time-course) 실험에이용될수 있다.

公开(公告)号：KR1020140017794A

公开(公告)日：2014-02-12

申请号：KR1020120084352

申请日：2012-08-01

Applicant: 대구가톨릭대학교산학협력단

Inventor： 김태완 ,  구본철 ,  권모선

IPC: C07K19/00 ,  C12N15/62 ,  C12N15/867

Abstract: The present invention relates to: a green fluorescent protein (GFP) characterized by having GFP properties so as to release unique fluorescence under light radiation having a wavelength of 480 nm and secret to the outside after passing a cell membrane when expressing, and thereby, simply being able to observe the expression thereof by irradiating ultraviolet rays of a long wavelength in a living cell, tissue, or animal if a sGFP gene is used as a reporter gene, or the transformed protein thereof and a recombinant protein of FSHB signal protein of a rat; a gene encoding the same; and a vector for expressing the gene. According to the present invention, an expression amount can be quantifized in a state in which a cell is alive without crushing the cell, and therefore, the present invention can be used in a time-course experiment.

Abstract translation: 本发明涉及：绿色荧光蛋白（GFP），其特征在于具有GFP特性，以便在波长为480nm的光辐射下释放独特的荧光，并且在表达之后通过细胞膜后对外界是秘密的，因此简单地 如果使用sGFP基因作为报告基因，则能够通过照射活细胞，组织或动物中的长波长的紫外线或其转化蛋白质和FSHB信号蛋白的重组蛋白质来观察其表达 鼠; 编码该基因的基因; 和用于表达该基因的载体。 根据本发明，可以在细胞活着而不破坏细胞的状态下定量表达量，因此本发明可以在时间过程实验中使用。