公开(公告)号：KR1020130093370A

公开(公告)日：2013-08-22

申请号：KR1020120014926

申请日：2012-02-14

Applicant: 대구가톨릭대학교산학협력단 ,  최상원

Inventor： 최상원 ,  김은옥 ,  이유진 ,  손우림

IPC: A23L19/00 ,  A23L1/30 ,  A61K8/97

CPC classification number: A23L19/03 ,  A23L33/105 ,  A23V2002/00 ,  A23V2200/302 ,  A23V2200/3262 ,  A23V2200/328 ,  A61K8/97 ,  A61K2800/85 ,  C12G3/02

Abstract: PURPOSE: A production method of anti-liver toxicity, ant-diabetic, anti-aging, and anti-oxidative dihydroflavonol is provided to mass produce the dihydroflavonol by fermenting mulberry wine. CONSTITUTION: Mulberries are fermented and aged to obtain mulberry wine. The mulberry wine is mixed with ionized water with the same amount. The mixed solution is mixed with ethyl acetate for fractioning and separating supernatant. The ethyl acetate fraction is dissolved in a 20% ethane solution, and settled for one day to obtain supernatant. The supernatant is passed through a Diaion HP-20 ion resin for absorbing a flavonol compound, eluted by using a 50% ethanol solution, and concentrated to obtain a mulberry wine extract. ODS-A and Sephadex LH-20 column chromatography are successively performed to the mulberry wine extract.

Abstract translation: 目的：提供抗肝毒性，抗糖尿病，抗衰老和抗氧化二氢黄酮醇的生产方法，通过发酵桑树酒大量生产二氢黄酮醇。 构成：桑树发酵和老化以获得桑berry酒。 桑叶酒与相同量的电离水混合。 将混合溶液与乙酸乙酯混合以分级和分离上清液。 将乙酸乙酯级分溶于20％乙烷溶液中，沉降1天，得到上清液。 将上清液通过Diaion HP-20离子树脂吸收黄酮醇化合物，使用50％乙醇溶液洗脱，浓缩得到桑树提取物。 依次对桑树提取物进行ODS-A和Sephadex LH-20柱层析。