公开(公告)号：KR1020130093372A

公开(公告)日：2013-08-22

申请号：KR1020120014928

申请日：2012-02-14

Applicant: 대구가톨릭대학교산학협력단 ,  최상원

Inventor： 최상원 ,  김은옥 ,  임현희

IPC: A61K36/605 ,  A61K36/60 ,  A61K31/045 ,  A61K31/05

CPC classification number: A61K8/97 ,  A61K36/605 ,  A61K2236/10 ,  A61K2236/19 ,  A61K2236/33 ,  A61Q19/02 ,  A61Q19/08 ,  Y10S514/844 ,  Y10S514/857

Abstract: PURPOSE: A method for preparing a mulberry branch extract is provided to produce a large amount of purified powder containing oxyresveratrol with anti-inflammatory and antiaging functions and to use trans-rasveratrol and moracin as a novel material for functional foods and cosmetic products. CONSTITUTION: A method for preparing a mulberry branch extract containing oxyresveratrol comprises the steps of: steaming mulberry branches for one hour and drying; adding 650 kg of a 75-95% ethanol solution to 100 kg of the dried mulberry branches and extracting by ultrasonic wave at 40-50°C for 12 hours; concentrating the extract by reduced pressure; dissolving the concentrate in a 80% ethanol solution and incubating at room temperature; removing precipitate, adding normal-hexane, and fractioning; removing an upper layer oil and concentrating the lower layer; adding a 20% ethanol solution to the defatted ethanol extract of mulberry branches and suspending; treating the defatted ethanol extract with 10% pectinase and hydrolyzing at 30-40°C for 12 hours to obtain a hydrolysate; fractioning the hydrolysate with an ethyl acetate solvent and concentrating the upper layer by reduced pressure; dissolving the concentrate using the 20% ethanol solution and adsorbing onto diaion HP-20 adsorption ion exchange column; washing the resultant with the 20% ethanol solution; and eluting the resultant with a 40% ethanol solution and concentrating. [Reference numerals] (AA) Dried mulberry branches(100kg); (BB) CNCNF(650kg, 80% ethanol solution, ultrasonic extractor, 50°C, 12 hours, twice); (CC) Filter; (DD) Filtered liquid; (EE, LL) Concentrate by reduced pressure; (FF) Ethanol extract of the mulberry branches(10kg); (GG) Re-dissolve(50kg, ethanol solution); (HH) Incubate day and night; (II) Supernatant; (JJ) Defatting(100kg, normal-hexane); (KK) Lower layer; (MM) Defatted ethanol extract of mulberry branches(8.5kg); (NN) Suspend(50kg, 20% ethanol solution); (OO) Enzyme treatment(4L, pectinex 5 XL, 30~40°C, 12 hours); (PP) Cooling; (QQ) Fraction with a solvent(100kg, ethyl acetate, twice); (RR) Dehydrate(10kg, sodium sulfate anhydrous); (SS) Filter and concentrate by reduced pressure; (TT) Ethyl acetate extract(2.1kg); (UU) Diaion HP-20 column chromatography; (VV) Fraction with 40% ethanol solution; (WW) Fraction with 60% ethanol solution; (XX) Mulberry branch extract I(1.2kg); (YY) Mulberry branch extract II(110g)

Abstract translation: 目的：提供一种桑树枝提取物的制备方法，以产生大量具有抗炎和抗衰老作用的含有羟基维生素的纯化粉末，并使用反式西伐他汀和莫拉辛作为功能性食品和化妆品的新型材料。 构成：含有羟基维生素的桑树分枝提取物的制备方法，包括：蒸桑桑干1小时，干燥; 向100公斤干桑树中加入650公斤75-95％乙醇溶液，超声波在40-50℃下萃取12小时; 减压浓缩提取物; 将浓缩物溶解在80％乙醇溶液中并在室温下孵育; 除去沉淀，加正己烷，分馏; 去除上层油并浓缩下层; 向桑树脱脂乙醇提取物中加入20％乙醇溶液悬浮; 用10％果胶酶处理脱脂乙醇提取物，并在30-40℃下水解12小时，得到水解产物; 用乙酸乙酯溶剂分级水解产物，减压浓缩上层; 使用20％乙醇溶液溶解浓缩物，并吸附在悬浮HP-20吸附离子交换柱上; 用20％乙醇溶液洗涤所得物; 并用40％乙醇溶液洗脱所得物并浓缩。 （附图标记）（AA）干桑树枝（100kg）; （BB）CNCNF（650kg，80％乙醇溶液，超声波提取器，50℃，12小时，两次）; （CC）过滤器; （DD）过滤液体; （EE，LL）减压浓缩; （FF）桑树分支乙醇提取物（10kg）; （GG）再溶解（50kg，乙醇溶液）; （HH）昼夜孵化; （二）上清液; （JJ）脱脂（100kg，正己烷）; （KK）下层; （MM）桑叶脱乙醇提取物（8.5kg）; （NN）悬浮液（50kg，20％乙醇溶液）; （OO）酶处理（4L，pectinex 5 XL，30〜40℃，12小时）; （PP）冷却; （QQ）用溶剂（100kg，乙酸乙酯，2次）分级; （RR）脱水（10kg，无水硫酸钠）; （SS）通过减压过滤和浓缩; （TT）乙酸乙酯提取物（2.1kg）; （UU）Diaion HP-20柱色谱; （VV）用40％乙醇溶液分级; （WW）用60％乙醇溶液分级; （XX）桑树枝提取物I（1.2kg）; （YY）桑树枝提取物II（110g）

公开(公告)号：KR101438543B1

公开(公告)日：2014-09-18

申请号：KR1020120014928

申请日：2012-02-14

Applicant: 대구가톨릭대학교산학협력단 ,  최상원

Inventor： 최상원 ,  김은옥 ,  임현희

IPC: A61K36/605 ,  A61K36/60 ,  A61K31/045 ,  A61K31/05

Abstract: 본 발명은 항염증 및 항노화성 옥시레스베라트롤, 트란스-레스베라트롤 및 모라신 함유 뽕나무가지(상지)추출물의 제조방법에 관한 것으로, 상지를 찜처리한 후 건조하고 80% 에탄올수용액으로 추출하여 얻은 에탄올추출물을 pectinex 효소처리한 후 에틸아세테이트로 분획하고 농축한 후 최종 다이아이온 HP-20 흡착이온수지 칼럼 크로마토그래피를 실시하여 항염증성 및 항노화성 옥시레스베라트롤 (oxyresveratrol), 트란스-레스베라트롤(
trans -resveratrol) 및 모라신(moracin)을 함유하는 상지추출물을 제조하고, 이의 피부 염증 치료제 및 피부미백 기능성화장품 신소재용도를 개시한다.

公开(公告)号：KR1020110121487A

公开(公告)日：2011-11-07

申请号：KR1020100041094

申请日：2010-04-30

Applicant: 대구가톨릭대학교산학협력단 ,  서미자

Inventor： 최상원 ,  김은옥 ,  임현희 ,  서미자

IPC: A61K36/14 ,  A61K36/264 ,  A61K8/97 ,  A61Q19/08

CPC classification number: A61K36/14

Abstract: PURPOSE: An oriental medicine material extract containing eugenol derivatives and a method for preparing the same are provided to ensure antioxidation, anti-aging, and anti-inflammation. CONSTITUTION: A method for preparing steam distillation extract of oriental medicine materials comprises: a step of drying herb medicines and adding distilled water; a step of adding ether and isolating with a steam distillation device; and a step of decompressing. A functional composition for antioxidation or antiaging contains the extract as an active ingredient. A functional composition for anti-inflammation contains the extract as an active ingredient. The extract contains eugenol derivatives as an active ingredient. The eugenol derivative is methyleugenol or acetyleugenol.

Abstract translation: 目的：提供含有丁子香酚衍生物的东方药物提取物及其制备方法，以确保抗氧化，抗衰老和抗炎。 构成：东方药材蒸汽蒸馏提取物的制备方法，包括：草药干燥，加入蒸馏水的步骤; 加入乙醚并用蒸汽蒸馏装置分离的步骤; 和解压缩的一个步骤。 用于抗氧化或抗衰老的功能组合物含有提取物作为活性成分。 用于抗炎的功能组合物含有提取物作为活性成分。 提取物含有丁子香酚衍生物作为活性成分。 丁子香酚衍生物是甲基丁烯醇或乙酰丁烯醇。

公开(公告)号：KR1020130000114A

公开(公告)日：2013-01-02

申请号：KR1020110060606

申请日：2011-06-22

Applicant: 서미자 ,  대구가톨릭대학교산학협력단

Inventor： 서미자 ,  최상원 ,  김은옥 ,  임현희

IPC: A61K36/234 ,  A61K36/232 ,  A61P29/00

Abstract: PURPOSE: An herbal mist for treating inflammation using a steam distilled extract of various medicinal herbs is provided to ensure excellent antioxidative and anti-inflammatory effects. CONSTITUTION: A cosmetic composition for preventing and treating skin inflammatory diseases contains a volatile perfume ingredient as an active ingredient, contained in a steam distilled extract of Cnidium officinale Makino and Angelica gigas Nakai. The volatile perfume ingredient is eugenol and acetyl eugenol. A health functional food and a cosmetic composition for preventing or treating skin inflammatory diseases contain the extract as an active ingredient. A functional cosmetic composition for antioxidation or anti-aging contains the extract as an active ingredient. [Reference numerals] (AA) Naturally drying medicinal herbs; (BB) Maturing in a traditional pot; (CC) Heating in a heating tank; (DD) Distillation in a distillation facility; (EE) Cooling in a cooling tank; (FF) Storing in a storage tank; (GG) Packaging in a container; (HH) Products

Abstract translation: 目的：提供使用各种药草蒸汽蒸馏提取物治疗炎症的草药雾，以确保优异的抗氧化和抗炎作用。 构成：用于预防和治疗皮肤炎性疾病的化妆品组合物含有作为活性成分的挥发性香料成分，其包含在C ale Mak a a a a a distilled distilled distilled。。。。。 挥发性香料成分是丁子香酚和乙酰丁香酚。 用于预防或治疗皮肤炎性疾病的健康功能食品和化妆品组合物含有提取物作为活性成分。 用于抗氧化或抗衰老的功能性化妆品组合物含有提取物作为活性成分。 [参考号]（AA）天然干草药; （BB）成熟在传统的锅; （CC）在加热罐中加热; （DD）蒸馏设备中的蒸馏; （EE）冷却箱中的冷却; （FF）储存在储罐中; （GG）包装在容器中; （HH）产品