公开(公告)号：KR101448890B1

公开(公告)日：2014-10-13

申请号：KR1020140061108

申请日：2014-05-21

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 신윤경 ,  명정인 ,  박미선 ,  김응오 ,  전제천 ,  민병화 ,  박경일

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6893 ,  C12Q1/6813

Abstract: The present invention relates to an inspecting kit and an inspecting method using a probe and a primer for inspecting Azumiobodo hoyamushi which are pathogenic protozoa causing a soft tunic syndrome in Halocynthia roretzi. The present invention relates to an inspecting kit (oligonucleotide microarray) and an inspecting method for rapidly detecting a pathogenic organism of a soft tunic syndrome in Halocynthia roretzi by producing a primer through a probe which is specific to a PCR amplification condition in which a 18S rDNA gene of Azumiobodo hoyamushi causing soft tunic syndrome in Halocynthia roretzi can be identified. Consequently, the Halocynthia roretzi of Azumiobodo hoyamushi which is known as the cause of soft tunic syndrome in Halocynthia roretzi is rapidly detected from seawater or Halocynthia roretzi from the initial symptoms of soft tunic syndrome, so that the inspecting kit and inspecting method are applied to an Azumiobodo hoyamushi soft tunic syndrome forecasting system, and damage can be preemptively prevented.

Abstract translation: 本发明涉及一种使用探针和底漆检查的检查试剂盒和检查方法，所述探针和底漆用于检查在Halocynthia roretzi中引起柔软综合征的病原性原虫的Azumiobodo hoyamushi。 本发明涉及一种检测试剂盒（寡核苷酸微阵列）及其检测方法，用于通过针对PCR扩增条件特异性的探针产生引物，从而快速检测出嗜热链球菌中软性综合征的致病生物体，其中将18S rDNA 可以鉴定出Halocynthia roretzi中引起软性综合征的Azumiobodo hoyamushi的基因。 因此，从软海绵综合征的初始症状中，海藻或卤虫属（Halocynthia roretzi）中迅速检出了被称为卤虫属（Halocynthia roretzi）柔软上皮综合征的原因的Azumiobodo hoyamushi的卤虫属（Halocynthia roretzi），从而将检测试剂盒和检查方法应用于 Azumiobodo hoyamushi软套综合征预测系统，并可以抢先防范损伤。