감태 유리배우체의 분리 배양 및 성숙 유도를 통한 감태 대량 생산 방법
    1.
    发明公开
    감태 유리배우체의 분리 배양 및 성숙 유도를 통한 감태 대량 생산 방법 有权
    用于分离和再生ECKLONIA CAVA的游戏性和通过成熟感应大量生产ECKLONIA CAVA的方法

    公开(公告)号:KR1020100093323A

    公开(公告)日:2010-08-25

    申请号:KR1020090012452

    申请日:2009-02-16

    Abstract: PURPOSE: A method for producing Ecklonia cava is provided to massively produce Eckolnia cava under the optimal culture condition and to protect environment for artificial cultivation. CONSTITUTION: A method for massively producing Ecklonia cava gametophytes comprises: a step of cutting Ecklonia cava apothecium part to induce release of zoospores; a step of geminating released zoospores in a liquid medium to form gametophytes; a step of separating gametophytes to male and female; a step of culturing the female and male gametophytes in a liquid medium; and a step of homogenizing the female and male gametophytes to cut in 100-200 um; and a step of culturing the cut gametophytes at 10-20°C, 5 ~ 40u molm^-2s^-1 of lightness, and 14:10(L:D). The culture medium is PESI(Provasoli's enriched seawater media).

    Abstract translation: 目的:提供Ecklonia Cava的生产方法,在最佳培养条件下大量生产Eckolnia cava,保护人工栽培环境。 构成:大规模生产Ecklonia Cava配子体的方法包括:切割Ecklonia cava apothecium部分以诱发游动孢子释放的步骤; 在液体介质中引发释放的游动孢子以形成配子体的步骤; 将配子体分为男性和女性的步骤; 在液体培养基中培养雌性和雄性配子体的步骤; 将雌配子和雄配子的同质化在100-200 um的步骤; 在10-20℃,5〜40u molm ^ -2s ^ -1的亮度和14:10(L:D)下培养切割的配子体的步骤。 培养基是PESI(Provasoli的富含海水介质)。

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