公开(公告)号：KR101329316B1

公开(公告)日：2013-11-15

申请号：KR1020130105485

申请日：2013-09-03

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 신윤경 ,  명정인 ,  전제천 ,  홍연철 ,  송수민 ,  이용석 ,  정지은

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12N15/11 ,  C12Q1/6844

Abstract: The present invention provides a test method for quickly identifying infection by a small amount of Azumiobodo hoyamushi using loop-mediated isothermal gene amplification (LAMP). In order to develop a high sensitive test method using an LAMP reaction, the sensitivity and the specificity in Azumiobodo hoyamushi and Halocynthia roretzi with soft tunic syndrome are analyzed by preparing a primer set for LAMP containing six primers to target kinetoplast 18S ribosome genes of Azumiobodo hoyamushi and amplify a reactant with a specific loop type; and establishing an optimal reaction condition. Thus, Azumiobodo hoyamushi which is known for causing soft tunic syndrome is quickly detected from sea water or Halocynthia roretzi with the initial stage soft tunic syndrome using the LAMP reaction and damage spread is early detected by applying the method to a system for predicting soft tunic syndrome in farmed Halocynthia roretzi.

Abstract translation: 本发明提供了使用环介导的等温基因扩增（LAMP）快速鉴定少量Azumiobodo hoyamushi的感染的测试方法。 为了开发使用LAMP反应的高灵敏度试验方法，通过制备含有6个引物的LAMP引物，分析了Azumiobodo hoyamushi和Halocynthia roretzi与柔软上皮综合征的敏感性和特异性，以靶向Azumiobodo hoyamushi的细胞质18S核糖体基因 并扩增具有特定环类型的反应物; 并建立最佳反应条件。 因此，使用LAMP反应，使用LAMP反应从海水或Halocynthia roretzi快速检测到已知引起软性综合征的Azumiobodo hoyamushi，并通过将该方法应用于预测软性综合征的系统来早期检测到 在养殖的Halocynthia roretzi。