公开(公告)号：KR100379021B1

公开(公告)日：2003-04-08

申请号：KR1020000009915

申请日：2000-02-28

Applicant: 대한민국(관리부서:농촌진흥청)

Inventor： 정명근 ,  백인열 ,  강성택 ,  송석보 ,  신두철 ,  곽용호 ,  김순철

IPC: A23L1/272

Abstract: PURPOSE: A method for separating natural coloring matter, anthocyanin from the testa of vegetable seed such as black bean is provided to process anthocyanin including delphinidin(formular3) and cyanidin(formular4) in high purity. CONSTITUTION: The method includes the following steps of: (i) soaking the testa of vegetable seed in water containing 0.1-1% hydrochloric acid and getting water soluble crude extract of anthocyanin for 24-72hr 2-4 times; (ii) removing disused materials from the extract by filtrating the crude extract of anthocyanin; (iii) concentrating the crude extract by putting the extract into an inverted column made of silica gel; (iv) isolating the crude extract of anthocyanin with 10-30% alcohol and hydrolyzing the isolated anthocyanin by adding acid thereto; and (v) filtrating the hydrolyzed solution and processing the anthocyanin by using a silica gel inverted cartridge.

Abstract translation: 用途：提供从黑豆等蔬菜种子的种皮中分离天然色素的方法，以高纯度处理花色素，包括花翠素（formular3）和花青素（formular4）。 组成：该方法包括以下步骤：（i）将蔬菜种子的种皮浸泡在含有0.1-1％盐酸的水中，并使花色素苷的水溶性粗提取物浸泡24-72小时2-4次; （ii）通过过滤花色素苷的粗提取物从提取物中除去废物; （iii）通过将提取物放入由硅胶制成的倒置柱中浓缩粗提物; （iv）用10-30％醇分离花青素的粗提取物并通过向其中加入酸水解分离的花色素苷; 和（v）过滤水解的溶液并通过使用硅胶转化柱处理花青素。

公开(公告)号：KR1020010084705A

公开(公告)日：2001-09-06

申请号：KR1020000009915

申请日：2000-02-28

Applicant: 대한민국(관리부서:농촌진흥청)

Inventor： 정명근 ,  백인열 ,  강성택 ,  송석보 ,  신두철 ,  곽용호 ,  김순철

IPC: A23L1/272

CPC classification number: A23L5/41 ,  A23L5/23

Abstract: PURPOSE: A method for separating natural coloring matter, anthocyanin from the testa of vegetable seed such as black bean is provided to process anthocyanin including delphinidin(formular3) and cyanidin(formular4) in high purity. CONSTITUTION: The method includes the following steps of: (i) soaking the testa of vegetable seed in water containing 0.1-1% hydrochloric acid and getting water soluble crude extract of anthocyanin for 24-72hr 2-4 times; (ii) removing disused materials from the extract by filtrating the crude extract of anthocyanin; (iii) concentrating the crude extract by putting the extract into an inverted column made of silica gel; (iv) isolating the crude extract of anthocyanin with 10-30% alcohol and hydrolyzing the isolated anthocyanin by adding acid thereto; and (v) filtrating the hydrolyzed solution and processing the anthocyanin by using a silica gel inverted cartridge.

Abstract translation: 目的：提供一种从黑豆等植物种子的睾丸中分离出天然色素，花色素苷的方法，以高纯度处理花青素，包括花翠素（formular3）和花青素（formular4）。 方法：该方法包括以下步骤：（1）将蔬菜种子浸泡在含有0.1-1％盐酸的水中，得到花青素水溶性粗提物24-72小时2-4次; （ii）通过过滤花青素的粗提取物从提取物中除去废弃的物质; （iii）通过将提取物放入由硅胶制成的倒置柱中来浓缩粗提取物; （iv）用10-30％的醇分离花青素的粗提物，并通过向其中加酸分解花青素分离; 和（v）使用硅胶倒置的滤筒过滤水解的溶液并加工花青素。