公开(公告)号：KR1020070079198A

公开(公告)日：2007-08-06

申请号：KR1020060009731

申请日：2006-02-01

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 정상희 ,  박수정 ,  구현옥 ,  강환구 ,  조준형

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158 ,  C12Q1/6837

Abstract: A DNA chip is provided to evaluate the harm to human body caused by changing of human flora, which is prepared by identifying and selecting 90 kinds of genes which significantly react to the change of the human flora in colon crypt cell of human flora-associated mice and amplifying and purifying the genes. The DNA chip for detecting specific reaction of human flora comprises at least one gene selected from a gene table(A) attached to a substrate, wherein the gene table(A) consists of Dap3, Rpa1, Ccnd2, Cdc45l, Gmnn, Cul4b, Tacc2, Bnip3l, Slc26a1, Ddx1, Cacnb3, Rpl27, Slc22a1l, Cav, Tuba4, Lrp10, Mt-1, Tiam2, Zdhhc3, Rhcg, Ipo4, 2610529I12Rik, Igj, Daf1, Il18, Tnfsf13b, Prkiri, Serping1, Eif2ak3, Mpp1, Lnx1, 4732481h14Rik, Rgs12, Dcamkl1, Mllt1, Per1, Keap1, Hdac5, Ncoa6, Crem, Crsp7, Rnf12, Alas1, Cotl11, Gstm2, Siat9, 5430437P03Rik, Purb, Col3a1, Il16, Mut, 6330590F17Rik, Srpk2, Klf3, Cited1, D230019K24Rik,, Ugcg, Au043625, Rw1-pending, Hsd17b2, 5031404N19, Tccr, Npm1, Sh3glb2, Aldh6a1, Plp, Ptgs1, Fads3, Parva, C130039O16, Epc1, CPd, Mtmr7, 4930455F23Rik, Rab6ip1, Mcpt4, Fn3k, 1110037F02Rik, 1110038M16Rik, 170012H12H17Rik, Thsd1, 98301480O20Rik, Ptgs2, Tcp11, Guca1a, Usp15, Ybx2, Tcl1, Cldn8 and Ckap2. The method comprises the steps of: (a) reacting the DNA chip with RNA which is collected from colon crypt cells and amplified; and (b) investigating the gene expression degree of the DNA chip.

Abstract translation: 提供DNA芯片以评估由人类菌群变化引起的对人体的伤害，其通过鉴定和选择与人类菌群相关小鼠的结肠隐窝细胞中的人类菌群的变化显着反应的90种基因而制备 并扩增和纯化基因。 用于检测人类菌群特异性反应的DNA芯片包含至少一种选自附着于底物的基因表（A）的基因，其中基因表（A）由Dap3，Rpa1，Ccnd2，Cdc451，Gmnn，Cul4b，Tacc2组成 ，Bnip3I，Slc26a1，Ddx1，Cacnb3，Rpl27，Slc22a1，Cav，Tuba4，Lrp10，Mt-1，Tiam2，Zdhhc3，Rhcg，Ipo4,2610529I12Rik，Igj，Daf1，Il18，Tnfsf13b，Prkiri，Serping1，Eif2ak3，Mpp1，Lnx1 ，4732481h14Rik，Rgs12，Dcamkl1，Mllt1，Per1，Keap1，Hdac5，Ncoa6，Crem，Crsp7，Rnf12，Alas1，Cotl11，Gstm2，Siat9,5430437P03Rik，Purb，Col3a1，Il16，Mut，6330590F17Rik，Srpk2，Klf3，Cited1，D230019K24Rik ，Ugcg，Au043625，Rw1-pending，Hsd17b2,5031404N19，Tccr，Npm1，Sh3glb2，Aldh6a1，Plp，Ptgs1，Fads3，Parva，C130039O16，Epc1，CPd，Mtmr7,4930455F23Rik，Rab6ip1，Mcpt4，Fn3k，1110037F02Rik，1110038M16Rik， 170012H12H17Rik，Thsd1,98301480O20Rik，Ptgs2，Tcp11，Guca1a，Usp15，Ybx2，Tcl1，Cldn8和Ckap2。 该方法包括以下步骤：（a）使DNA芯片与从结肠隐窝细胞收集的RNA进行反应并扩增; 和（b）研究DNA芯片的基因表达程度。

公开(公告)号：KR100809945B1

公开(公告)日：2008-03-05

申请号：KR1020060009731

申请日：2006-02-01

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 정상희 ,  박수정 ,  구현옥 ,  강환구 ,  조준형

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158

Abstract: The present invention relates to a DNA chip showing specific responses to a human intestinal normal bacterial flora and a method for estimating harmness to the human bodies due to the change of the human intestinal normal bacterial flora using the DNA chip.