公开(公告)号：KR100424137B1

公开(公告)日：2004-03-24

申请号：KR1020000068693

申请日：2000-11-18

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 허문 ,  조성근 ,  조동희 ,  정병열

IPC: C07K14/23 ,  G01N33/50

Abstract: PURPOSE: Provided are 8kDa antigen derived from Brucella abortus RB51, its purification method and a detection method of antibody for RB51 using the same antigen. CONSTITUTION: 8kDa antigen is derived from the surface of Brucella abortus RB51 and purified by the steps of: treating Brucella abortus RB51 with sarcosine to extract antigens from its surface; concentrating the extract with ammonium sulfate; dissolving the concentrate in triton X-100 then passing it through Superose 12HR 10/30 column; then passing the fractions through Sephacryl S-100.

Abstract translation: 用途：提供源自布鲁杆菌流产RB51的8kDa抗原，其纯化方法和使用相同抗原的RB51抗体的检测方法。 构成：8kDa抗原源自布鲁氏菌流产RB51的表面，并通过以下步骤纯化：用肌氨酸处理布鲁氏菌流产RB51以从其表面提取抗原; 用硫酸铵浓缩提取物; 将浓缩物溶解在Triton X-100中，然后将其通过Superose 12HR 10/30柱; 然后将馏分通过Sephacryl S-100。

公开(公告)号：KR1020020038844A

公开(公告)日：2002-05-24

申请号：KR1020000068693

申请日：2000-11-18

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 허문 ,  조성근 ,  조동희 ,  정병열

IPC: C07K14/23 ,  G01N33/50

CPC classification number: C07K14/23 ,  C07K1/145 ,  C07K1/16 ,  G01N33/536

Abstract: PURPOSE: Provided are 8kDa antigen derived from Brucella abortus RB51, its purification method and a detection method of antibody for RB51 using the same antigen. CONSTITUTION: 8kDa antigen is derived from the surface of Brucella abortus RB51 and purified by the steps of: treating Brucella abortus RB51 with sarcosine to extract antigens from its surface; concentrating the extract with ammonium sulfate; dissolving the concentrate in triton X-100 then passing it through Superose 12HR 10/30 column; then passing the fractions through Sephacryl S-100.

Abstract translation: 目的：提供来源于流产布鲁杆菌RB51的8kDa抗原，其纯化方法和使用相同抗原的RB51抗体检测方法。 构成：8kDa抗原来源于流产布鲁杆菌RB51的表面，并通过以下步骤纯化：用肌氨酸处理布鲁氏菌流产RB51，从其表面提取抗原; 用硫酸铵浓缩提取物; 将浓缩物溶解在triton X-100中，然后将其通过Superose 12HR 10/30柱; 然后将级分通过Sephacryl S-100。