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1.发明公开비단향꽃무 세균마름병균 검출용 ＰＣＲ 프라이머 쌍 및 이를 이용한 비단향꽃무 세균마름병균 검출 방법 无效用于检测XANTHOMONAS CAMPESTRIS PV的聚合酶链反应的聚合物组。 用于检测XANTHOMONAS CAMPESTRIS PV的麻醉剂和方法 使用相同的INCANAE
公开(公告)号：KR1020120053831A
公开(公告)日：2012-05-29
申请号：KR1020100115146
申请日：2010-11-18
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12Q1/04 , C12R1/64
Abstract: PURPOSE: A PCR primer pair for detecting Xanthomonas campestris pv. inc is provided to ensure specific sequence to Xanthomonas campestris pv. inc-specific gene. CONSTITUTION: A PCR primer pair for detecting Xanthomonas campestris pv. inc is denoted by sequence numbers 1 and 2 or 3 and 4. A composition for detecting Xanthomonas campestris pv. inc contains the primer pair. A kit for detecting Xanthomonas campestris pv. inc contains the primer pair. A method for detecting Xanthomonas campestris pv. inc comprises: a step of isolating DNA from a sample; a step of amplifying the DNA by PCR using the primer pair; and a step of detecting the PCR product by electrophoresis.
Abstract translation: 目的：检测野油菜黄单胞菌 提供inc，以确保对黄单胞菌（Xanthomonas campestris）pv。 特异性基因。 构成：用于检测野油菜黄单胞菌的PCR引物对 inc由序列号1和2或3和4表示。用于检测野油菜黄单胞菌（Xanthomonas campestris） inc包含引物对。 用于检测野油菜黄单胞菌的试剂盒 inc包含引物对。 野油菜黄单胞菌检测方法 包括：从样品中分离DNA的步骤; 使用引物对通过PCR扩增DNA的步骤; 以及通过电泳检测PCR产物的步骤。
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2.发明授权카로신 Ｄ 및 이를 생산하여 분비하는 펙토박테리움 카로토보룸 ＰＣＣ２１-Ｍ１５ 有权Carocin D和Carotovorum subsp。 carotovorum pcc21-M15产生和分泌所述的carocin D
公开(公告)号：KR101006084B1
公开(公告)日：2011-01-07
申请号：KR1020080092869
申请日：2008-09-22
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 새롭게 확인한 박테리오신 카로신 D 및 이를 생산하여 분비하는 펙토박테리움 카로토보룸(
Pectobacterium
carotovorum subsp.
carotovorum ) Pcc21-M15에 관한 것으로, 경제적이고 효과적으로 작물의 무름병 방제를 위해 사용될 수 있다.
박테리오신, 펙토박테리움, 무름병-
公开(公告)号：KR1020040047337A
公开(公告)日：2004-06-05
申请号：KR1020020075498
申请日：2002-11-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: Specific primers for detecting zucchini yellow mosaic virus are provided, thereby increasing the detection limitation and sensitivity about 1,000 times higher than using antiserum, detecting all kinds of zucchini yellow mosaic virus, and improving the detection accuracy by removing nonspecific reaction with other related viruses. CONSTITUTION: The specific primers for detecting zucchini yellow mosaic virus comprise a nucleic acid fragment selected from the nucleotide sequence set forth in SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO: 5 which species specifically binds with zucchini yellow mosaic virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof; and another nucleic acid fragment selected from the nucleotide sequence set forth in SEQ ID NO: 7 or SEQ ID NO: 8 which is combined with the selected nucleic acid and species specifically binds with zucchini yellow mosaic virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof, wherein the mutation is selected from deletion of base, addition of base, substitution of base or the combination thereof.
Abstract translation: 目的：提供用于检测西葫芦黄花叶病毒的特异性引物，从而提高检测限度和灵敏度，比使用抗血清高约1000倍，检测各种西葫芦黄花叶病毒，并通过去除与其他相关病毒的非特异性反应来提高检测精度 。 构成：用于检测西葫芦黄花叶病毒的特异性引物包含选自SEQ ID NO：2，SEQ ID NO：4或SEQ ID NO：5所示核苷酸序列的核酸片段，该核苷酸序列特异性结合西葫芦黄花叶病毒 ，其互补核苷酸序列及其突变核苷酸序列; 和选自SEQ ID NO：7或SEQ ID NO：8所示核苷酸序列的另一核酸片段，其与选择的核酸和物种组合特异性结合西葫芦黄花叶病毒，其互补核苷酸序列和突变核苷酸 序列，其中突变选自碱基的缺失，碱的加入，碱的取代或其组合。
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4.发明公开참깨 세균잎마름병균 검출용 ＰＣＲ 프라이머 쌍 및 이를 이용한 참깨 세균잎마름병균 검출 방법 有权用于检测XANTHOMONAS CAMPESTRIS PV的聚合酶链反应的聚合物组。 SESAMI和检测XANTHOMONAS CAMPESTRIS PV的方法。 SESAMI使用它
公开(公告)号：KR1020120053834A
公开(公告)日：2012-05-29
申请号：KR1020100115156
申请日：2010-11-18
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12Q1/04 , C12R1/64
Abstract: PURPOSE: A PCR primer pair for detecting Xanthomonas campestris pv. sesame is provided to diagnose and detect Xanthomonas campestris pv. sesame. CONSTITUTION: A primer pair for detecting Xanthomonas campestris pv. sesame is denoted by sequence numbers 1 and 2 or 3 and 4. A composition for detecting Xanthomonas campestris pv. sesame contains the primer pair. A kit for detecting Xanthomonas campestris pv. sesame contains the pair.
Abstract translation: 目的：检测野油菜黄单胞菌 提供芝麻诊断和检测野油菜黄单孢菌 芝麻。 构成：用于检测野油菜黄单胞菌的引物对 芝麻由序列号1和2或3和4表示。用于检测野油菜黄单胞菌（Xanthomonas campestris pv。）的组合物。 芝麻含有引物对。 用于检测野油菜黄单胞菌的试剂盒 芝麻包含这对。
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公开(公告)号：KR100496018B1
公开(公告)日：2005-06-16
申请号：KR1020020075498
申请日：2002-11-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본 발명은 쥬키니황화모자이크바이러스와 종 특이적으로 반응하는 핵산단편에 관한 것으로, 보다 상세하게는 서열번호 2,4,5,7,8로 표시되는 염기서열, 그의 상보적인 염기서열 또는 이들 염기서열에 의거한 변이가 실시된 변형서열의 어느 하나의 핵산단편 또는 이들 핵산 단편의 특정 조합으로 구성되는 프라이머를 개시한다.
본 발명에 의하면 항혈청을 이용한 진단방법보다 1,000배 이상 검출한계가 높으며, 지금까지 알려진 쥬키니항화모자이크바이러스의 모든 계통에 대한 검출이 가능하다. 또한 다른 관련 바이러스와의 비특이적 반응이 없어 훨씬 정밀하게 쥬키니황화모자이크바이러스의 감염여부를 진단할 수 있다.-
Patent Agency Ranking
公开(公告)号：KR1020040047338A
公开(公告)日：2004-06-05
申请号：KR1020020075499
申请日：2002-11-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/33
Abstract: PURPOSE: Specific primers for detecting pepper mild mottle virus are provided, thereby increasing the detection limitation and sensitivity about 1,000 times higher than using antiserum, detecting all kinds of pepper mild mottle virus, and improving the detection accuracy by removing nonspecific reaction with other related viruses. CONSTITUTION: The specific primers for detecting pepper mild mottle virus comprise a nucleic acid fragment selected from the nucleotide sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 4 which species specifically binds with pepper mild mottle virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof; and another nucleic acid fragment selected from the nucleotide sequence set forth in SEQ ID NO: 5 or SEQ ID NO: 6 which is combined with the selected nucleic acid and species specifically binds with pepper mild mottle virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof, wherein the mutation is selected from deletion of base, addition of base, substitution of base or the combination thereof.
Abstract translation: 目的：提供用于检测辣椒轻度斑驳病毒的特异性引物，从而提高检测限度和灵敏度，比使用抗血清高约1000倍，检测各种胡椒柔和斑驳病毒，并通过去除与其他相关病毒的非特异性反应来提高检测准确度 。 构成：用于检测辣椒柔和斑驳病毒的特异性引物包含选自SEQ ID NO：1或SEQ ID NO：4所示核苷酸序列的核酸片段，该核苷酸序列与辣椒柔和斑驳病毒特异性结合，其互补核苷酸序列， 及其突变核苷酸序列; 和选自与选择的核酸和物种组合的SEQ ID NO：5或SEQ ID NO：6所示核苷酸序列的另一个核酸片段与辣椒柔和斑驳病毒，其互补核苷酸序列和突变核苷酸特异性结合 序列，其中突变选自碱基的缺失，碱的加入，碱的取代或其组合。
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7.发明授权
公开(公告)号：KR101273270B1
公开(公告)日：2013-06-11
申请号：KR1020100115156
申请日：2010-11-18
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 참깨 세균잎마름병균(
Xanthomonas
campestris
pv
.
sesami ) 검출용 PCR 프라이머 쌍에 관한 것으로 보다 상세하게는 서열번호 1 및 서열번호 2 또는 서열번호 3 및 서열번호 4로 표시되는 PCR 프라이머 쌍, 상기 프라이머 쌍을 포함하는 참깨 세균잎마름병균 검출용 조성물 및 검출용 키트 및 상기 프라이머 쌍을 이용한 참깨 세균잎마름병균 미생물의 검출 방법에 관한 것이다. 본 발명의 프라이머 쌍은 참깨 세균잎마름병균에 특이적인 유전자에 특이적인 서열을 가지므로, 참깨 세균잎마름병균의 진단 및 검출에 사용될 수 있다.-
8.发明公开잔토모나스 속 미생물 검출용 ＰＣＲ 프라이머 쌍 및 이를 이용한 잔토모나스 속 미생물 검출 방법 无效用于检测基因XANTHOMONAS的聚合酶链反应的聚合物组和使用其的检测基因XANTHOMONAS的方法
公开(公告)号：KR1020120053790A
公开(公告)日：2012-05-29
申请号：KR1020100115089
申请日：2010-11-18
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12Q1/04 , C12R1/64
Abstract: PURPOSE: A PCR primer pair having a sequence which is specific to a specific gene in entire Xanthomonas sp. is provided to detect microorganism belonging to Xanthomonas sp. CONSTITUTION: A PCR primer pair for detecting Xanthomonas sp. microbes is denoted by sequence numbers 1 and 2. The primer pair is denoted by sequence numbers 3 and 4. A composition for detecting Xanthomonas sp. microbes contains the primer pair. A method for detecting Xanthomonas sp. comprises: a step of isolating DNA from a sample; a step of amplifying the DNA using the primer pair; and a step of detecting PCR product by electrophoresis.
Abstract translation: 目的：在整个黄单胞菌属中具有特定基因特异性序列的PCR引物对。 被用于检测属于黄单胞菌属的微生物。 构成：检测黄单胞菌属的PCR引物对 微生物由序列号1和2表示。引物对由序列号3和4表示。用于检测黄单胞菌属的组合物。 微生物含有引物对。 一种检测黄单胞菌属的方法 包括：从样品中分离DNA的步骤; 使用引物对扩增DNA的步骤; 以及通过电泳检测PCR产物的步骤。
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9.发明公开카로신 Ｄ 및 이를 생산하여 분비하는 펙토박테리움 카로토보룸 ＰＣＣ２１-Ｍ１５ 有权CAROCIN D和PUNGOBACTERIUM CAROTOVORUM SUBSP。 CAROTOVORUM PCC21-M15生产和分配SAID卡诺D
公开(公告)号：KR1020100033825A
公开(公告)日：2010-03-31
申请号：KR1020080092869
申请日：2008-09-22
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A Pectobacterium carotovorum Pcc21-M15 producing bacteriocin carocin D is provided to prevent bacterial soft rot and use microorganism agricultural chemical. CONSTITUTION: A Pectobacterium carotovorum Pcc21-M15(KACC91406) has bacteriocin activity. A gene encoding protein of sequence number 2 having bacteriocin activity has sequence of sequence number 1. A formulation for preventing bacterial soft rot contains Pectobacterium carotovorum Pcc21-M15 as an active ingredient. The formulation for preventing bacterial soft rot contains the protein having bacteriocin activity as an active ingredient. The formulation for preventing bacterial soft rot contains transformant transformed with the gene encoding the protein.
Abstract translation: 目的：提供致癌细菌素Caroctovorum Pcc21-M15产生细菌素Carocin D以防止细菌软腐病和使用微生物农药。 构成：Carotovorum Poc21-M15（KACC91406）具有细菌素活性。 编码具有细菌素活性的序列号2的蛋白质的基因具有序列号1的序列。用于防止细菌软腐烂的制剂含有作为活性成分的胡萝卜素Poc21-M15。 用于防止细菌软腐的制剂含有具有细菌素活性的蛋白质作为活性成分。 用于防止细菌软腐的制剂含有用编码蛋白质的基因转化的转化体。
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10.发明公开플라타너스 세균궤양병균 검출용 ＰＣＲ 프라이머 쌍 및 이를 이용한 플라타너스 세균궤양병균 검출 방법 无效用于检测XANTHOMONAS POPULI的聚合酶链反应的聚合物组和使用其的检测XANTHOMONAS POPULI的方法
公开(公告)号：KR1020120053832A
公开(公告)日：2012-05-29
申请号：KR1020100115148
申请日：2010-11-18
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12Q1/04 , C12R1/64
Abstract: PURPOSE: A PCR primer pair for detecting Xanthomonas populi is provided to diagnose and detect Xanthomonas populi. CONSTITUTION: A PCR primer pair for detecting Xanthomonas populi is denoted by sequence numbers 1 and 2 or 3 and 4. A kit for detecting Xanthomonas populi contains the primer pair. A method for detecting Xanthomonas populi comprises: a step of isolating DNA from a sample; a step of amplifying the DNA by PCR using the primer pair; and a step of detecting PCR products by electrophoresis.
Abstract translation: 目的：提供一种检测黄单胞病毒的PCR引物对，用于诊断和检测黄单胞菌（Hanthomonas populi）。 构成：用序列号1和2或3和4表示用于检测黄单胞菌属的PCR引物对。用于检测黄单胞杆菌的试剂盒含有引物对。 检测黄单胞菌的方法包括：从样品中分离DNA的步骤; 使用引物对通过PCR扩增DNA的步骤; 以及通过电泳检测PCR产物的步骤。
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