Abstract:
본 발명은 토바모바이러스 속 (Genus Tobamovirus) 바이러스에 특이적인 61개의 올리고뉴클레오티드 및 이의 상보적 올리고뉴클레오티드로 구성되는 군으로부터 선택되는 하나 이상의 올리고뉴클레오티드를 포함하는 토바모바이러스 속 바이러스 올리고뉴클레오티드 프로브 세트, 상기 올리고뉴클레오티드 프로브 세트를 포함하는 토바모바이러스 속 바이러스 진단 또는 검출용 유전자 칩, 토바모바이러스 속 바이러스에 감염된 것으로 추정되는 시료로부터 RNA를 분리하는 단계, 상기 분리된 RNA를 주형으로 하여 cDNA를 합성하는 단계, 상기 합성된 cDNA를 상기 올리고뉴클레오티드 프로브 세트와 접촉시켜 상기 cDNA와 프로브 세트를 혼성화시키는 단계 및 상기 프로브와 cDNA 사이의 혼성화 정도를 검출하는 단계를 포함하는 토바모바이러스 속 바이러스를 진단 또는 검출하는 방법 및 상기 올리고뉴클레오티드 프로브 세트 및 혼성화 용액을 포함하는 토바모바이러스 속 바이러스는 검출용 키트에 관한 것이다.
Abstract:
본 발명은 서열번호 1 내지 3882의 올리고뉴클레오티드 및 이의 상보적 올리고뉴클레오티드로 구성되는 군으로부터 선택되는 하나 이상의 올리고뉴클레오티드를 포함하는 식물 바이러스 진단 또는 검출용 올리고뉴클레오티드 프로브 세트, 상기 올리고뉴클레오티드 프로브 세트를 포함하는 식물 바이러스 진단 또는 검출용 유전자 칩, 식물 바이러스에 감염된 것으로 추정되는 시료로부터 RNA를 분리하는 단계, 상기 분리된 RNA를 주형으로 하여 cDNA를 합성하는 단계, 상기 합성된 cDNA를 올리고뉴클레오티드 프로브 세트와 접촉시켜 상기 cDNA와 프로브 세트를 혼성화시키는 단계 및 상기 프로브와 cDNA 사이의 혼성화 정도를 검출하는 단계를 포함하는 식물 바이러스를 진단 또는 검출하는 방법 및 상기 올리고뉴클레오티드 프로브 세트 및 혼성화 용액을 포함하는 식물 바이러스 진단 또는 검출용 키트에 관한 것이다.
Abstract:
PURPOSE: A probe set for diagnosing or detecting PLRV(potato leaf roll virus) is provided to minimize damage due to viral diseases in agricultural products and to enhance production. CONSTITUTION: A probe set for diagnosing or detecting PLRV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 2025-2027, 2031-2035, and 2048-2056, and complementary oligonucleotides thereof. A method for diagnosing or detecting PLRV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA; a step of hybridizing the cDNA with the probe set; and a step of detecting the hybridization. [Reference numerals] (AA) Potato; (BB) Host: Potato; (CC) Host RNA con.: None
Abstract:
PURPOSE: A probe set for diagnosing or detecting TRSV(tobacco ringspot virus) is provided to minimize damage by viral diseases in agricultural products and to enhance production. CONSTITUTION: An oligonucleotide for diagnosing or detecting TRSV contains one or more oligonucleotides selected from a group consisting of oligonucleotides of sequence numbers 800, 1781-1786, and 1809-1821 and complementary oligonucleotides thereof. A method for diagnosing or detecting TRSV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA from RNA; a step of hybridizing the synthesized cDNA with the probe set; and a step of detecting hybridization of the cDNA and the probe set. [Reference numerals] (AA) Jangyeobkong; (BB) Host: Jangyeobkong
Abstract:
PURPOSE: A probe set for diagnosing or detecting SBMV(Southern bean mosaic virus) is provided to minimize damage to crops due to viral diseases and to enhance the yield of crops. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting SBMV contains one or more oligonucleotides selected from a group consisting of oligonucleotides of sequence numbers 3024-3028 and 3057-3060 and complementary oligonucleotides thereof. A method for diagnosing or detecting SBMV comprises: a step of isolating RNS and synthesizing cDNA; a step of hybridizing the cDNA and the probe set; and a step of detecting the hybridization of the cDNA and the probe set. [Reference numerals] (AA) Jangwonkong; (BB) Host: Jangwonkong
Abstract:
PURPOSE: A probe set for diagnosing or detecting PSTVd(Potato spindle tuber viroid) is provided to minimize damage due to viral diseases in crops and to enhance production. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting PSTVd(Potato spindle tuber viroid) contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 2122-2125 and 2131 or complementary oligonucleotides thereof. A method for diagnosing or detecting PSTVd comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA; a step of hybridizing the cDNA and the probe set; and a step of detecting hybridization of the probe and cDNA. [Reference numerals] (AA) Potato; (BB) Host: Potato; (CC) Host RNA con.: None
Abstract:
PURPOSE: A Probe set for diagnosing or detecting Squash mosaic virus is provided to increase production amount by minimizing influence on crops by viruses. CONSTITUTION: A Probe set for diagnosing or detecting SqMV(Squash mosaic virus) comprises one or more oligonucleotids selected from oligonucleotid with a sequence number 894-909 or complementary oligonucleotid thereof. A method for diagnosing or detecting the SqMV comprises a step of separating RNA from a specimen; a step of synthesizing cDNA from the RNA; a step of contacting the cDNA to the probe set to mix the cDNA and probe set; and a step of detecting hybridization degree between the cDNA and probe set. An SqMV diagnosing or detecting kit comprises the oligonucleotid probe set and hybridization solution. [Reference numerals] (AA) Green pumpkin; (BB) Host: Green pumpkin
Abstract:
PURPOSE: A probe set for diagnosing Genus Potyvirus is provided to minimize damage to crops and to improve product yields. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting Genus Potyvirus contains one or more oligonucleotides selected from a group consisting of oligonucleotides of sequence numbers 2309, 2332, 2350-2355, 2361-2365, 2375-2377, 2382-2387, 2392, 2441-2445, 2450-2454, 2455-2468, 2480-2481, 2484-2492, 2495-2499, 2616-2624, 2699-2708, 2729-2734, 2765-2772, 2791-2800, 2848-2856, 2858, 2877, and 2915-2923; and a complementary oligonucleotide thereof. Genus Potyvirus includes TuMV(Turnip mosaic virus), WMV2(Watermelon mosaic virus 2), SMV(Soybean mosaic virus), ZYMV(Zucchini yellow mosaic virus), PRSV(Papaya ringspot virus), PepMoV(Pepper mottle virus), LMV(Lettuce mosaic virus), or PVY(Potato virus Y). A gene chip for diagnosing or detecting Genus Potyvirus contains the oligonucleotide probe set. [Reference numerals] (AA) Radish; (BB) Host: Giljomoo
Abstract:
PURPOSE: A novel cynanchum wilfordii hemsley mosaic virus discovered from a cynanchum plant, a primer for detecting the same and a detecting method of the cynanchum wilfordii hemsley mosaic virus using the same are provided to enhance the detection efficiency of the novel virus by using a primer pair. CONSTITUTION: A cynanchum wilfordii hemsley mosaic virus gene includes base sequence describe in the sequence number 1(SEQ ID NO:1). A primer pair for detecting cynanchum wilfordii hemsley mosaic virus comprises a forward primer selected from primers described in the sequence number 2-4 and a reverse primer selected from pimers described in the sequence number 5-7. The Cynanchum wilfordii Hemsley mosaic virus includes the base sequence described in the sequence number 1. A detecting method of the Cynanchum wilfordii Hemsley mosaic virus comprises the following steps: separating RNA from a sample; performing RT-PCR by using the primer pair as a mold; and detecting the virus from the sample by analyzing the PCR product. The Cynanchum wilfordii Hemsley mosaic virus detecting kit includes the primer pair.
Abstract translation:目的:提供一种从猕猴桃植物发现的一种新型猕猴桃il ley ley ley mosaic mosaic mosaic mosaic,,,,,,a primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer primer 对。 构成:猕猴桃ms ii花叶病毒基因包括序列号1(SEQ ID NO:1)中描述的碱基序列。 用于检测枸杞子叶片花叶病毒的引物对包括选自序列号2-4中所述的引物的正向引物和选自序列号5-7中描述的引物的反向引物。 Cynanchum wilfordii Hemsley花叶病毒包括序列号1中描述的碱基序列。Cynanchum wilfordii Hemsley花叶病毒的检测方法包括以下步骤:从样品中分离RNA; 通过使用引物对作为模具进行RT-PCR; 并通过分析PCR产物从样品中检测病毒。 Cynanchum wilfordii Hemsley花叶病毒检测试剂盒包括引物对。