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公开(公告)号：KR1020160058222A
公开(公告)日：2016-05-25
申请号：KR1020140153005
申请日：2014-11-05
Applicant: 을지대학교 산학협력단 , 대한민국(농촌진흥청장)
IPC: A23L33/00 , A61K36/899 , C07D311/62
CPC classification number: A23L33/00 , A23V2200/3262 , A23V2250/2104 , A23V2250/308 , A61K36/899 , C07D311/62
Abstract: 본발명은흑미호분세포층으로부터안토시아닌추출방법에관한것이다.
Abstract translation: 本发明涉及从黑米糊粉细胞层提取花青素的方法。 从黑米提取花青素的方法包括使用50％乙醇提取黑米粉糊粉细胞层的步骤。 功能性物质如从黑米糊粉细胞层获得的花青素可用作肥胖和骨骼健康的功能食品材料。
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2.发明授权애멸구 유래 재조합 그로이엘단백질 유전자 클론 및 이를이용한 벼줄무늬 잎마름병 바이러스의 면역포획역전사효소연쇄중합반응 검정방법 有权来自小褐斑病病人的重组体基因克隆和使用蛋白质蛋白质的米糠病毒免疫测定RT-PCR的检测方法
公开(公告)号：KR100822742B1
公开(公告)日：2008-04-17
申请号：KR1020070026577
申请日：2007-03-19
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6888 , C12Q1/701 , C12Q2531/113
Abstract: A recombinant protein derived from small brown planthopper is provided to be specifically coupled to thread-shaped rice stripe virus, thereby forecasting precise RSV outbreak, developing a transgenic plant having RSV resistance and being applied as various tools for detecting the RSV. A recombinant protein described in SEQ ID : NO. 2 is obtained by expressing a recombinant protein in a gene clone(deposition no. KACC-95060P) described in SEQ ID : NO. 1, which is constructed by inserting a GroEL gene isolated from small brown planthopper into an expression vector, and then purifying the protein using His-taq Ni-NTA agarose column and is characterized in that it is specifically coupled to thread-shaped RSV. A method for detecting an RSV gene comprises the steps of: (a) coating a reaction container which is PolySorp treated with the GroEL recombinant protein derived from the small brown planthopper or an antibody thereof with a coating solution; (b) treating the reaction solution with a detecting sample through coupling reaction with the with the RSV to immuno-capturing RSV particles; and (c) after isolating genes from the immuno-captured RSV particles, subjecting the isolated genes to RT-PCR to detect genes of the RSV.
Abstract translation: 提供了一种来源于小型褐飞虱的重组蛋白，特异性地与丝状水稻条纹病毒偶联，从而预测了精确的RSV疫情，开发了具有RSV抗性的转基因植物，并被用作检测RSV的各种工具。 SEQ ID NO： 2是通过在SEQ ID NO：1中描述的基因克隆（沉积编号KACC-95060P）中表达重组蛋白获得的。 1，其通过将从小褐飞虱分离的GroEL基因插入表达载体中构建，然后使用His-taq Ni-NTA琼脂糖柱纯化蛋白质，其特征在于其特异性结合于螺纹状RSV。 检测RSV基因的方法包括以下步骤：（a）用来自小褐飞虱或其抗体的GroEL重组蛋白用涂布溶液涂覆PolySorp处理的反应容器; （b）用检测样品通过与RSV的偶联反应处理反应溶液以免疫捕获RSV颗粒; 和（c）从免疫捕获的RSV颗粒中分离出基因后，将分离的基因进行RT-PCR检测RSV的基因。
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3.发明公开버티실리움 레카니 씨에스626 국내 토착균주 및 이를이용한 시설해충의 방제방법 有权MICROORGANISM VERTICILLIUM LECANII CS626及使用该方法控制有害物质的方法
公开(公告)号：KR1020020076646A
公开(公告)日：2002-10-11
申请号：KR1020010016649
申请日：2001-03-29
Applicant: 대한민국(농촌진흥청장) , 윤철식
IPC: C12N1/20
Abstract: PURPOSE: A microorganism Verticillium lecanii CS626 and a method for the control of harmful insects using the same microorganism are provided, thereby effectively preventing the growth of the harmful insects living in agricultural equipments. CONSTITUTION: The microorganism Verticillium lecanii CS626(KFCC-11277) has an improved insecticidal effect on the harmful insects living in agricultural equipments, wherein it is isolated by collecting a mycelium from the surface of an insect infected by Verticillium lecanni; inoculating the mycelium on a potato agar medium containing tetracycline; culturing it at 22 to 28 deg. C; isolating spores and mycelia from the cultured medium; suspending the isolated spores in 0.01% tween 80 solution; spraying the suspension on a leaf containing insects; and selecting a microorganism showing excellent insecticidal activity.
Abstract translation: 目的：提供微生物轮枝孢菌CS626和使用相同微生物控制害虫的方法，从而有效防止生活在农业设备中的有害昆虫的生长。 构成：微生物轮枝孢属植物CS626（KFCC-11277）对农业设备中的有害昆虫具有改善的杀虫作用，其通过从轮枝堇病毒感染的昆虫表面收集菌丝来分离， 将菌丝体接种在含有四环素的马铃薯琼脂培养基上; 在22〜28度培养 C; 从培养基中分离孢子和菌丝体; 将分离的孢子悬浮在0.01％吐温80溶液中; 将悬浮液喷洒在含有昆虫的叶上; 并选择显示出优异的杀虫活性的微生物。
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公开(公告)号：KR1020130015726A
公开(公告)日：2013-02-14
申请号：KR1020110077891
申请日：2011-08-04
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A microbial agent containing Bacillus subtilis R2-1 or a culture medium thereof for preventing powdery mildew, and a prevention method using the same are provided to effectively prevent powdery mildew with an eco-friendly method. CONSTITUTION: A microbial agent for preventing powdery mildew contains Bacillus subtilis R2-1(deposit number: KACC 91542P) or a culture medium thereof. The culture medium contains rice or barley. The culture medium also contains: a liquid fertilizer of fish amino acid, humic acid, and molasses in a ratio of 1:8-12:8-12, or a liquid fertilizer of fish amino acid, glucose, and molasses in a ratio of 1:3-7:8-12; 1-3 wt% of molasses, 0.1-3 wt% of glucose, and 0.01-0.5 wt% of soytone; and an organic fertilizer which contains 4-6 wt% of nitrogen, 1-3 wt% of phosphoric acid, 0.3-2 wt% of potassium(40-50 wt% of caster bean waste, 30-50 wt% of rape seed waste, 8-12 wt% of rice bran, and 3-7 wt% of palm waste). The microbial agent prevents Rhizoctonia solani, Phytophthora capsici, Fusarium oxysporum, or Colletotrichum acutatum. A method for preventing powdery mildew includes a step of treating a plant with the microbial agent. Powdery mildew is caused by Leveillula taurica or Erysiphe cichoracearum. [Reference numerals] (AA) Non-treated; (BB) Weight(g) per week; (CC) Number(piece) per week
Abstract translation: 目的：提供含有枯草芽孢杆菌R2-1或其培养基的用于预防白粉病的微生物剂及其预防方法，以通过环保方法有效预防白粉病。 构成：用于预防白粉病的微生物剂含有枯草芽孢杆菌R2-1（保藏号：KACC 91542P）或其培养基。 培养基含有大米或大麦。 培养基还含有：以氨基酸，葡萄糖和糖蜜的比例为1：8-12：8-12的鱼氨基酸，腐植酸和糖蜜的液体肥料， 1：3-7：8-12; 1-3重量％的糖蜜，0.1-3重量％的葡萄糖和0.01-0.5重量％的大豆黄酮; 和含有4-6重量％氮，1-3重量％磷酸，0.3-2重量％钾（40-50重量％的脚轮豆废弃物，30-50重量％的油菜籽废物）的有机肥料 ，8-12重量％的米糠和3-7重量％的棕榈废料）。 微生物剂防止茄子，丝状疫霉，尖孢镰刀菌或or螨。 预防白粉病的方法包括用微生物剂处理植物的步骤。 白粉病是由金银花（Leveillula taurica）或白粉病（Erysiphe cichoracearum）引起的。 （附图标记）（AA）未处理; （BB）每周重量（g） （CC）每周数量（件）
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公开(公告)号：KR101293640B1
公开(公告)日：2013-08-07
申请号：KR1020110077891
申请日：2011-08-04
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 바실러스 서브틸리스 균주 R2-1 (수탁번호: KACC91542P) 또는 그의 배양액을 포함하는 흰가루병 방제용 미생물 제제, 및 이를 이용하여 흰가루병을 방제하는 방법에 관한 것이다. 본 발명에 따르면, 대량생산된 바실러스 서브틸리스 균주 R2-1을 이용하여 환경친화적이고 효과적으로 흰가루병을 방제할 수 있다.
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Patent Agency Ranking
公开(公告)号：KR100475346B1
公开(公告)日：2005-03-10
申请号：KR1020020040749
申请日：2002-07-12
Applicant: 대한민국(농촌진흥청장) , 학교법인고려중앙학원
IPC: C12N1/14
Abstract: 본 발명은 국내 토착 균주
버티실리움 레카니 CS625(
Verticillium lecanii CS625) 및 이를 이용한 진딧물 방제 방법에 관한 것이다. 보다 구체적으로는, 진딧물에 대하여 살충력이 우수하고 시설 재배지 환경조건에서도 잘 적응된 국내 토착 균주
버티실리움 레카니 CS625 균주, 상기 균주 및 균주의 포자 현탁액을 함유하는 진딧물 방제용 조성물 및 상기 조성물을 농작물에 살포하여 진딧물, 특히 목화진딧물 및 복숭아혹진딧물을 생물학적으로 방제하는 방법에 관한 것이다. 본 발명 균주는 복숭아혹진딧물로부터 분리한 국내 토착 균주로, 시설 재배지의 환경조건에서도 생장률이 우수할 뿐만 아니라, 상기 균주 및 균주로부터 생성된 포자현탁액을 시설 재배재의 농작물에 처리 시 목화진딧물을 효과적으로 방제할 수 있는 뛰어난 효과가 있다.-
7.发明公开
公开(公告)号：KR1020040006457A
公开(公告)日：2004-01-24
申请号：KR1020020040749
申请日：2002-07-12
Applicant: 대한민국(농촌진흥청장) , 학교법인고려중앙학원
IPC: C12N1/14
Abstract: PURPOSE: A microorganism Verticillium lecanii CS625 and a method for the control of aphids using the same are provided. The microorganism has improved growth rate in protected cultivation and effectively controls aphids in crops being grown by the protected cultivation. CONSTITUTION: A microorganism Verticillium lecanii CS625(KACC 93002) is provided. A composition for the control of aphids comprises the spore suspension produced from Verticillium lecanii CS625(KACC 93002) as an effective component, wherein the spore suspension comprises conidium or blastopore produced from Verticillium lecanii CS625(KACC 93002); and the spore suspension contains 10(6) to 10(10) cfu/ml of spores. A method for the control of aphids comprises spraying the aphid controlling composition to crops.
Abstract translation: 目的：提供微生物轮枝孢菌CS625和使用其的蚜虫控制方法。 微生物在受保护的栽培中具有改善的生长速度，并有效控制受保护栽培种植的作物中的蚜虫。 构成：提供了一种微生物轮枝孢菌TC625（KACC 93002）。 用于控制蚜虫的组合物包含作为有效成分的由轮枝孢霉属植物轮枝孢菌CS625（KACC 93002）产生的孢子悬浮液，其中孢子悬浮液包含由轮枝孢虱CS625（KACC 93002）产生的分生孢子或孢子虫; 孢子悬浮液含有10（6）至10（10）cfu / ml孢子。 用于控制蚜虫的方法包括将蚜虫控制组合物喷洒到作物上。
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公开(公告)号：KR101706295B1
公开(公告)日：2017-02-13
申请号：KR1020140153004
申请日：2014-11-05
Applicant: 을지대학교 산학협력단 , 대한민국(농촌진흥청장)
IPC: A23L33/00 , A61K36/899 , C07D311/62
Abstract: 본발명은흑미로부터안토시아닌추출방법에관한것이다.
Abstract translation: 本发明涉及一种从黑米中提取花青素的方法，其中通过使用日本杏提取物和发酵精液的混合提取溶剂，该方法可以提高黑米花青素的提取效率。 此外，从黑米提取花青素的方法包括使用50％乙醇和热水的混合萃取溶剂萃取黑米的步骤。
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公开(公告)号：KR1020160058221A
公开(公告)日：2016-05-25
申请号：KR1020140153004
申请日：2014-11-05
Applicant: 을지대학교 산학협력단 , 대한민국(농촌진흥청장)
IPC: A23L33/00 , A61K36/899 , C07D311/62
CPC classification number: A23L33/00 , A23V2200/308 , A23V2200/3262 , A23V2250/2104 , A61K36/899 , C07D311/62
Abstract: 본발명은흑미로부터안토시아닌추출방법에관한것이다.
Abstract translation: 本发明涉及一种从黑米中提取花青素的方法，其中通过使用日本杏提取物和发酵精液的混合提取溶剂，该方法可以提高黑米花青素的提取效率。 此外，从黑米提取花青素的方法包括使用50％乙醇和热水的混合萃取溶剂萃取黑米的步骤。
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公开(公告)号：KR100404767B1
公开(公告)日：2003-11-07
申请号：KR1020010016649
申请日：2001-03-29
Applicant: 대한민국(농촌진흥청장) , 윤철식
IPC: C12N1/20
Abstract: PURPOSE: A microorganism Verticillium lecanii CS626 and a method for the control of harmful insects using the same microorganism are provided, thereby effectively preventing the growth of the harmful insects living in agricultural equipments. CONSTITUTION: The microorganism Verticillium lecanii CS626(KFCC-11277) has an improved insecticidal effect on the harmful insects living in agricultural equipments, wherein it is isolated by collecting a mycelium from the surface of an insect infected by Verticillium lecanni; inoculating the mycelium on a potato agar medium containing tetracycline; culturing it at 22 to 28 deg. C; isolating spores and mycelia from the cultured medium; suspending the isolated spores in 0.01% tween 80 solution; spraying the suspension on a leaf containing insects; and selecting a microorganism showing excellent insecticidal activity.
Abstract translation: 目的：提供一种微生物蜡蚧轮枝菌（Verticillium lecanii）CS626和使用相同微生物控制有害昆虫的方法，从而有效地防止住在农业设备中的有害昆虫的生长。 组成：微生物蜡蚧轮枝菌Verticillium lecanii CS626（KFCC-11277）对农业设备中的有害昆虫具有改善的杀虫效果，其中通过从被轮枝孢菌感染的昆虫表面收集菌丝体来分离它; 将菌丝体接种在含有四环素的马铃薯琼脂培养基上; 在22至28度培养它。 C; 从培养基中分离孢子和菌丝体; 将分离的孢子悬浮在0.01％吐温80溶液中; 将悬浮液喷洒在含有昆虫的叶上; 并选择显示优异的杀虫活性的微生物。
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