公开(公告)号：KR1020120109069A

公开(公告)日：2012-10-08

申请号：KR1020110026512

申请日：2011-03-24

Applicant: 대한민국(농촌진흥청장) ,  경상대학교산학협력단

Inventor： 조인철 ,  김재환 ,  정하연 ,  고문석 ,  이성수 ,  전진태 ,  임현태 ,  유채경 ,  서보영

IPC: C12N15/12 ,  C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2600/124

Abstract: PURPOSE: A marker for detecting swine hair color and a detection method are provided to develop a technique for removing a factor generating hair color. CONSTITUTION: A marker for detecting swine hair color contains SNP or copy number variation(CNV) of swine KIT gene having 123th base of sequence number 5 in which G is substituted with A and a mixture thereof. The pig is Landrace, Yorkshire, or Large White. A kit contains the marker. A method for detecting the swine hair color factor comprises: a step of preparing a nucleic acid sample from a subject; a step of identifying base sequence of SNP and CNV of swine KIT gene; and a step of determining high probability in a pig containing a SNP of swine KIT gene at 123th base of sequence number 5 is G/G without CNV of KIT gene.

Abstract translation: 目的：提供用于检测猪头发颜色的标记和检测方法，以开发用于去除产生头发颜色的因子的技术。 构成：用于检测猪毛发颜色的标记物含有SNP或拷贝数变异（CNV），其具有序列号5的123位的碱基KIT基因，其中G被A取代，其混合物。 猪是兰德拉斯，约克郡或大白人。 套件包含标记。 检测猪毛发色素的方法包括：从受试者制备核酸样品的步骤; 识别猪KIT基因的SNP和CNV的碱基序列; 并且在序列号5的123碱基的猪KIT基因的SNP中测定高概率的步骤是没有KIT基因的CNV的G / G。

公开(公告)号：KR101008941B1

公开(公告)日：2011-01-17

申请号：KR1020070100513

申请日：2007-10-05

Applicant: 대한민국(농촌진흥청장) ,  경상대학교산학협력단

Inventor： 윤두학 ,  전진태 ,  문희주 ,  임현태

IPC: C12Q1/68

Abstract: 본 발명은 여러 소 품종 내에서 세 개의 멀티플렉싱 피씨알 시스템(multiplexing PCR system)을 이용하여 다중의 서로 다른 유전자 좌위를 동시에 다중 증폭시킨 후, 각각의 초위성체 좌위(microsatellite loci)의 대립유전자 형질을 측정하여 한우 및 수입우를 판별하는 방법에 관한 것이다.
한우, 품종식별, 멀티플렉싱 피씨알 시스템, 수입우육

公开(公告)号：KR1020120108524A

公开(公告)日：2012-10-05

申请号：KR1020110026504

申请日：2011-03-24

Applicant: 대한민국(농촌진흥청장) ,  경상대학교산학협력단

Inventor： 조인철 ,  김재환 ,  정하연 ,  고문석 ,  이성수 ,  전진태 ,  임현태 ,  유채경 ,  서보영

IPC: C12N15/12 ,  C12Q1/68 ,  A01K67/027

CPC classification number: C12Q1/6827 ,  A01K67/0275 ,  C12Q2600/124

Abstract: PURPOSE: A method for producing human gray hair model animal using pigs is provided to analyze KIT genotype and to develop novel drugs for preventing gray hair. CONSTITUTION: A marker for detecting pig roan expression factor contains pig KIT gene having G or A at 123th base of sequence number 3 and E locus of MC1R gene. The pig is landrace and Korean native pig. A method for detecting pig roan expression factor comprises: a step of preparing a nucleic acid sample from a subject; a step of identifying SNP of the pig KIT gene and E locus of MC1R gene of pig; and a step of determining the roan pig expression by crossbreeding the landrace and Korean native pig when the landrace is EP, and Korean native pig is ED1/ED1, ED1/ED2, or ED2/ED2.

Abstract translation: 目的：提供使用猪生产人类灰发模型动物的方法，以分析KIT基因型，开发用于预防灰白发的新药。 构成：检测猪an表达因子的标记物含有在序列号123的碱基和MC1R基因的E位点具有G或A的猪KIT基因。 猪是地方品种和韩国本地猪。 检测猪流感表达因子的方法包括：从受试者制备核酸样品的步骤; 识别猪的KIT基因和猪MC1R基因的E位点的SNP的步骤; 韩国本地猪为ED1 / ED1，ED1 / ED2或ED2 / ED2时，通过杂交育种地方品种和韩国本地猪，确定猪猪表达的步骤。

公开(公告)号：KR1020090035302A

公开(公告)日：2009-04-09

申请号：KR1020070100513

申请日：2007-10-05

Applicant: 대한민국(농촌진흥청장) ,  경상대학교산학협력단

Inventor： 윤두학 ,  전진태 ,  문희주 ,  임현태

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2537/143

Abstract: A method for discrimination between Korean beef cattle and imported cattle using a multiplexing PCR and a primer therefore are provided to distinguish species of cattle by analyzing gene loci rapidly and accurately. A method for discrimination between Korean beef cattle and imported cattle using a multiplexing PCR comprises the following steps of: obtaining DNA of cattle having two pairs of primers and a plurality of gene loci; amplifying a base sequence comprising each locus of a DNA sample into a multiplexing PCR; and measuring characteristic form and quality of an allele of each microsatellite locus.

Abstract translation: 提供了使用复用PCR和引物来区分韩国肉牛和进口牛的方法，以通过快速且准确地分析基因位点来区分牛的种类。 使用复用PCR鉴别韩国肉牛和进口牛的方法包括以下步骤：获得具有两对引物和多个基因座的牛的DNA; 将包含DNA样品的每个位点的碱基序列扩增到多重PCR中; 并测量每个微卫星位点等位基因的特征形式和质量。

公开(公告)号：KR1020070066352A

公开(公告)日：2007-06-27

申请号：KR1020050127392

申请日：2005-12-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 윤두학 ,  김종주 ,  전진태 ,  박응우 ,  이승환 ,  오성종

IPC: C12Q1/68 ,  G06F19/28

Abstract: A method for identifying Korean native cattle is provided to distinguish the Korean native cattle from imported beef with excellent accuracy by using microsatellite loci, thereby improving income of a farmhouse raising the Korean native cattle and the reliability of consumers. The method comprises the steps of: (a) preparing a PCR reaction solution for amplifying alleles corresponding to microsatellite loci of a selected cow such as ILSTS005, ILSTS028, ILSTS103, ILSTS006, ILSTS008, ILSTS023, ILSTS050, TGLA122, TGLA126, MGTG4B, TGLA227, BM1824, BM2112, ETH10, ETH225, ETH3, and SPS115; (b) amplifying the alleles by performing the multiple PCR reaction thereon using a PCR reaction solution which is a combination of ETH225, ETH10, ETH3, BM2113, BM1824 and SPS115; (c) isolating each of the amplified alleles and then analyzing the size of the each of the alleles; (d) putting the size of the alleles by individuals and species in order and preparing the number and the frequency distribution of the alleles by the species based thereon; and (e) building an information bank of the alleles corresponding to the microsatellite loci to identify the species according to the statistical analysis.

Abstract translation: 提供了一种识别韩国本地牛的方法，通过使用微卫星位点，以极高的准确度将韩国本地牛与进口牛肉区分开来，从而提高了养殖韩国本地牛的农场收入和消费者的可靠性。 该方法包括以下步骤：（a）制备PCR反应溶液，用于扩增相应于所选择的牛的微卫星位点的等位基因，例如ILSTS005，ILSTS028，ILSTS103，ILSTS006，ILSTS008，ILSTS023，ILSTS050，TGLA122，TGLA126，MGTG4B，TGLA227， BM1824，BM2112，ETH10，ETH225，ETH3和SPS115; （b）通过使用作为ETH225，ETH10，ETH3，BM2113，BM1824和SPS115的组合的PCR反应溶液在其上进行多重PCR反应来扩增等位基因; （c）分离每个扩增的等位基因，然后分析每个等位基因的大小; （d）按个人和物种排列等位基因的大小，并根据物种准备等位基因的数量和频率分布; 和（e）建立与微卫星位点相对应的等位基因的信息库，以根据统计分析鉴定该物种。