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公开(公告)号：KR100307669B1
公开(公告)日：2001-09-24
申请号：KR1019990005703
申请日：1999-02-20
Applicant: 대한민국(농촌진흥청장)
IPC: C12N7/00
Abstract: 본발명은백합잠재바이러스(Lily symptomless virus, LSV)검정용단크론항체를분비하는융합세포인하이브리도마(Hybridoma) 와하이브리도마에의해분비되는단크론항체에관한것이다. 백합잠재바이러스는백합을재배하고있는세계어느지역에서나보편적으로감염되어있으며, 이러한바이러스감염이고품질절화백합생산은물론, 백합생산을위한우량종구생산의제한요인이되고있다. 본발명은세포융합기술을이용하여백합잠재바이러스에대해특이적으로반응하는단크론항체를개발하여대량의시료에대한바이러스의감염여부를검정할수 있도록하고, 종래의항체시약의수입에따른비경제성과수입시수송도중에야기되는변질, 적기공급등의제반문제들을해결할수 있다.
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公开(公告)号：KR1020000056410A
公开(公告)日：2000-09-15
申请号：KR1019990005703
申请日：1999-02-20
Applicant: 대한민국(농촌진흥청장)
IPC: C12N7/00
Abstract: PURPOSE: A hybridoma and a monoclonal antibody secreted by the hybridoma for assaying Lily symptomless virus (LSV) is provided. The monoclonal antibody is designed to specifically react with the LSV using the cell fusion technique and can be used in assaying the virus infection in a large scale of sample. CONSTITUTION: A hybridoma secreting a monoclonal antibody to the LSV is prepared by comprising the following steps of: purifying the LSV; designing, screening and cloning the hybridoma exhibiting genetic properties of a lymphocyte (BALB/c) and a tumor cell (NS-1) by fusing the lymphocyte which is obtained in an animal immune reaction and the tumor cell; purifying the monoclonal antibody for assaying the LSV; identifying the isotype of the antibody by ELISA; identifying that the monoclonal antibody can recognize the epitope of the LSV by dot immunobinding assay (DIBA); assaying the LSV infection. The prepared hybridoma 1G6-B1 line is deposited in Korean Cell Line Bank, under the deposit number KCLRF-BP-00021.
Abstract translation: 目的：提供杂交瘤和用于测定百合无症状病毒（LSV）的杂交瘤分泌的单克隆抗体。 单克隆抗体被设计为使用细胞融合技术与LSV特异性反应，并且可用于测定大规模样品中的病毒感染。 构成：通过以下步骤制备分泌LSV的单克隆抗体的杂交瘤：纯化LSV; 通过融合在动物免疫反应中获得的淋巴细胞和肿瘤细胞，设计，筛选和克隆显示淋巴细胞（BALB / c）和肿瘤细胞（NS-1）的遗传性质的杂交瘤; 纯化用于测定LSV的单克隆抗体; 通过ELISA鉴定抗体的同种型; 识别单克隆抗体可以通过点免疫结合测定（DIBA）识别LSV的表位; 测定LSV感染。 制备的杂交瘤1G6-B1系保藏在韩国细胞系银行，保藏号为KCLRF-BP-00021。
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