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公开(公告)号：KR100832522B1
公开(公告)日：2008-05-27
申请号：KR1020060053631
申请日：2006-06-14
Applicant: 대한민국(농촌진흥청장) , 케이맥(주)
Abstract: 본 발명에 따른 카바메이트계 및 유기인계 농약 성분의 검출방법은 i) 금속박막 칩의 표면을 활성화시켜 주는 단계, ii) pH 3.5 내지 5.0의 pH 버퍼 용액으로 항체를 희석하여 항체 희석액을 준비하는 단계, iii)상기 항체 희석액을 상기 표면에 흘려주어 상기 금속박막 표면에 항체를 고정화시키는 단계 및 iv) 상기 표면에 아민계 화합물을 제공하여 상기 항체를 안정화시키는 단계를 포함하는 항체 고정화 단계; 검출 대상 시료를 상기 항체가 고정화된 금속박막 칩 상에 제공하는 단계; 상기 검출 대상 시료를 분석하는 시료 분석 단계; 및 상기 검출 대상 시료를 상기 금속박막 칩으로부터 해리시키는 해리 단계를 포함한다.
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公开(公告)号：KR1020110006334A
公开(公告)日：2011-01-20
申请号：KR1020090063918
申请日：2009-07-14
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , G01N33/52 , C12Q1/06
Abstract: PURPOSE: A method for measuring the amount of bacteria using a nano biosensor is provided to quickly measure the number of bacteria with high sensitivity. CONSTITUTION: A method for measuring the number of bacteria comprises: a step of isolating bacteria group in a sample; a step of inputting antibody-fixed nano biosensor to the bacteria group to label only bacteria; a step of removing unlabeled bacteria; a step of measuring the number of bacteria in the sample using fluorescence intensity generated in the nano biosensor. The step of isolating bacteria group comprises: a step of inputting magnetic particles having antibodies on the surface; and a step of separating bacteria using a magnet.
Abstract translation: 目的：提供一种使用纳米生物传感器测量细菌量的方法，以快速测量细菌数量，具有高灵敏度。 构成：用于测量细菌数量的方法包括：分离样品中的细菌组的步骤; 向细菌群体输入抗体固定的纳米生物传感器以仅标记细菌的步骤; 去除未标记细菌的步骤; 使用在纳米生物传感器中产生的荧光强度来测量样品中细菌数量的步骤。 分离菌群的步骤包括：在表面上输入具有抗体的磁性颗粒的步骤; 以及使用磁铁分离细菌的步骤。
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公开(公告)号：KR101149418B1
公开(公告)日：2012-06-27
申请号：KR1020090063918
申请日：2009-07-14
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , G01N33/52 , C12Q1/06
Abstract: PURPOSE: A method for measuring the amount of bacteria using a nano biosensor is provided to quickly measure the number of bacteria with high sensitivity. CONSTITUTION: A method for measuring the number of bacteria comprises: a step of isolating bacteria group in a sample; a step of inputting antibody-fixed nano biosensor to the bacteria group to label only bacteria; a step of removing unlabeled bacteria; a step of measuring the number of bacteria in the sample using fluorescence intensity generated in the nano biosensor. The step of isolating bacteria group comprises: a step of inputting magnetic particles having antibodies on the surface; and a step of separating bacteria using a magnet.
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公开(公告)号：KR1020070119251A
公开(公告)日：2007-12-20
申请号：KR1020060053631
申请日：2006-06-14
Applicant: 대한민국(농촌진흥청장) , 케이맥(주)
Abstract: A method for detecting carbamate-based and organic phosphate-based agricultural chemicals is provided to secure excellent detection sensitivity and shorten the detection time, thereby significantly improving the detecting efficiency. A method for detecting carbamate-based and organic phosphate-based agricultural chemicals comprises the steps of: (a) activating the surface of a metallic thin film chip by allowing N-hydroxysuccinimide and [N-ethyl-N'(dimethylaminopropyl)carbodiimide] to flow on the surface thereof; (b) diluting an antibody using a pH buffer solution with a pH of 3.5-5.0 to prepare a diluted solution of the antibody; (c) allowing the diluted solution to flow on the surface of the chip to fix the antibody on the surface of the chip; (d) providing an amine-based compound to the surface to stabilize the antibody; (e) providing a sample to be detected to the antibody fixed metallic thin film chip; (f) analyzing the sample; and (g) dissociating the sample from the metallic thin film chip, wherein the metallic thin film chip is made of Au and the antibody is glutathione s-transferase(GST) or acetylcholinesterase(ACHE).
Abstract translation: 提供了一种检测氨基甲酸酯类和有机磷酸酯类农药的方法，以确保优异的检测灵敏度并缩短检测时间，从而显着提高检测效率。 检测氨基甲酸酯类和有机磷酸酯类农药的方法包括以下步骤：（a）通过使N-羟基琥珀酰亚胺和[N-乙基-N'（二甲基氨基丙基）碳二亚胺]活化金属薄膜芯片的表面 在其表面上流动; （b）使用pH为3.5-5.0的pH缓冲溶液稀释抗体以制备抗体的稀释溶液; （c）使稀释溶液流过芯片表面以将抗体固定在芯片的表面上; （d）向表面提供胺类化合物以稳定抗体; （e）向所述抗体固定金属薄膜芯片提供要检测的样品; （f）分析样品; 和（g）从金属薄膜芯片解离样品，其中金属薄膜芯片由Au制成，抗体是谷胱甘肽转移酶（GST）或乙酰胆碱酯酶（ACHE）。
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