公开(公告)号：KR100344571B1

公开(公告)日：2002-08-19

申请号：KR1019990033412

申请日：1999-08-13

Applicant: 대한민국(농촌진흥청장)

Inventor： 홍영호 ,  정일정 ,  김태헌 ,  윤두학 ,  김희발 ,  김형선

IPC: C12Q1/68

Abstract: 본발명에의한한우고기판별용에스씨에이알프라이머는, 5'에서 3' 방향으로 CCA GAG CCT CGG GGA CTG AC의염기서열을갖는제1 프라이머와, 5'에서 3' 방향으로 CGC CCC CAG TAC ACC ATG AG의염기서열을갖는제2 프라이머로이루어지며, 상기제1 프라이머는임의의소의 DNA의 5'에부착되고, 상기제2 프라이머는상기 DNA의 3'에부착되는것을특징으로한다. 상기와같은구성에따르면, 한우고기에만특이밴드가나타나도록하되젖소고기에서는특이밴드가나타나지않도록함으로써, 신속정확하게한우고기를판별해낼수 있게된다.

公开(公告)号：KR1020010017747A

公开(公告)日：2001-03-05

申请号：KR1019990033412

申请日：1999-08-13

Applicant: 대한민국(농촌진흥청장)

Inventor： 홍영호 ,  정일정 ,  김태헌 ,  윤두학 ,  김희발 ,  김형선

IPC: C12Q1/68

Abstract: PURPOSE: Provided is an SCR(Sequence Characterized amplified Regions) primer for distinguishing Korean beef meat from milk cow meat, wherein band peculiar to Korean beef meat is appeared not in milk cow meat but in Korean beef meat. The SCR primer distinguishes them quickly and correctly. CONSTITUTION: A SCR primer is composed of the first primer having base sequence of CCA GAG CCT CGG GGA CTG AC in the direction from 5' toward 3' and the second primer having base sequence of CGC CCC CAG TAC ACC ATG AG in the direction from 5' toward 3'. The distinguishing method uses SCR primers, wherein the first primer is attached to 5' end of DNA of cow to be checked and the second primer is attached to 3' end of DNA of cow to be checked.

Abstract translation: 目的：提供一种用于区分韩国牛肉和牛奶肉的SCR（序列特征扩增区）引物，其中韩国牛肉特有的条纹不在牛奶肉中，而是在韩国牛肉中。 SCR引物快速正确地区分它们。 构成：SCR引物由具有CCA GAG CCT CGG GGA CTG AC的碱基序列的第一引物在5'至3'的方向组成，第二引物由CGC CCC CAG TAC ACC ATG AG的碱基序列沿着 5'朝3'。 区分方法使用SCR引物，其中第一引物连接到待检查的母牛的DNA的5'末端，第二引物连接到待检查的母牛的3'末端。