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Patent Agency Ranking
公开(公告)号：KR100870802B1
公开(公告)日：2008-11-27
申请号：KR1020080057981
申请日：2008-06-19
Applicant: 동국대학교 산학협력단
Abstract: 본 발명은 토양 친화 미생물의 자체 순환 시스템을 이용한 식물성장 촉진방법에 관한 것으로, 구체적으로 1) 경작지로부터 채취한 토양 시료를 해당 경작지의 토양을 함유한 배지에 집적 배양하여 토양 친화 미생물을 분리하는 단계; 2) 분리된 토양 친화 미생물의 복합 배양체를 제조하는 단계; 3) 상기 복합 배양체를 해당 경작지에 살포하여 살포된 토양 친화 미생물을 우점화시키는 단계; 및 4) 상기 단계 1), 2) 및 3)을 순환 반복하는 단계를 포함하는, 식물성장 촉진방법에 관한 것이다. 본 발명에 따른 식물성장 촉진방법은 토양 친화 미생물의 자체 순환 시스템을 이용하기 때문에 환경오염을 유발하지 않으면서 각종 식물의 성장을 촉진할 수 있어 생산성 증대 및 유기농업을 위한 농업기술의 개선, 오염토양 복원 등에 유용하게 사용될 수 있다.
Abstract translation: 本发明涉及一种促进利用土壤亲和力微生物的自循环系统的植物生长的方法，具体而言，1）该方法包括综合培养来自于含有土壤的土地分离土壤亲和力微生物的培养基中培养收集的土壤样品 。 2）制备分离的土壤友好微生物的组合培养物; 3）在耕地上喷洒复合培养物以控制喷洒的土壤微生物; 4）重复上述步骤1），2）和3）。 根据本发明农业技术植物的改进生长促进方法，而不会造成环境污染，因为它使用土壤友好微生物其自身的循环系统，以促进各种植物的生长提高生产率和有机农业那里，污染土壤 恢复等
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公开(公告)号：KR100849407B1
公开(公告)日：2008-07-31
申请号：KR1020070056146
申请日：2007-06-08
Applicant: 동국대학교 산학협력단
Abstract: 본 발명은 토양 친화 미생물의 자체 순환 시스템을 이용한 식물성장 촉진방법에 관한 것으로, 구체적으로 1) 경작지로부터 채취한 토양 시료를 해당 경작지의 토양을 함유한 배지에 집적 배양하여 토양 친화 미생물을 분리하는 단계; 2) 분리된 토양 친화 미생물의 복합 배양체를 제조하는 단계; 3) 상기 복합 배양체를 해당 경작지에 살포하여 살포된 토양 친화 미생물을 우점화시키는 단계; 및 4) 상기 단계 1), 2) 및 3)을 순환 반복하는 단계를 포함하는, 식물성장 촉진방법에 관한 것이다. 본 발명에 따른 식물성장 촉진방법은 토양 친화 미생물의 자체 순환 시스템을 이용하기 때문에 환경오염을 유발하지 않으면서 각종 식물의 성장을 촉진할 수 있어 생산성 증대 및 유기농업을 위한 농업기술의 개선, 오염토양 복원 등에 유용하게 사용될 수 있다.
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公开(公告)号：KR1020080059543A
公开(公告)日：2008-06-30
申请号：KR1020080057981
申请日：2008-06-19
Applicant: 동국대학교 산학협력단
CPC classification number: A01N63/02 , A01N25/02 , A01N25/04 , A01N25/08 , A01N25/14 , C12N1/20 , C12R1/05
Abstract: A method for isolating soil-friendly microorganisms from a cultivated land is provided to isolate the microorganisms with excellent soil-affinity from the cultivated land to increase productivity of an environment-friendly agricultural product by spraying a complex culture of the microorganisms onto the cultivated land, thereby promoting the plant growth. A method for isolating soil-friendly microorganisms from a cultivated land comprises the steps of: (a) mixing a soil sample of a land for cultivating a plant with saline solution; (b) adding a soil sample of the same land to a minimum culture medium to prepare a minimum culture medium for isolating soil-friendly microorganisms; (c) inoculating the mixture obtained from the step(b) into the minimum culture medium prepared from the step(b); (d) culturing an inoculation solution obtained from the step(c) at a temperature of 25-30 deg.C for 15-20 days; (e) after diluting a culture solution obtained from the step(d) with saline solution, smearing a culture medium for isolation with the diluted solution and culturing it at a temperature of 25-30 deg.C for 5-7 days; and (f) isolating colonies formed on the culture medium as the soil friendly microorganisms of the corresponding land, wherein the minimum culture medium for isolation includes 0.1-0.2 weight/volume% of NH4Cl, 0.44-0.54 weight/volume% of K2HPO4, 0.4-0.5 weight/volume% of NaH2PO4.2H2O, 0.05-0.06 weight/volume% of MgSO4.7H2O, 0.0003-0.0004 weight/volume% of CaCl2.2H2O, 0.0001-0.0002 weight/volume% of FeSO4.7H2O, 0.0001-0.0002 weight/volume% of MnCl2.4H2O, 0.00001-0.00002 weight/volume% of CoCl2.6H2O, 0.00001-0.00002 weight/volume% of Na2MoO4.2H2O and 0.00004-0.00005 weight/volume% of monohydrated citric acid and the culture medium for isolation includes 0.4-0.5 wt.% of peptone, 0.2-0.3 wt.% of a beef extract and 1.0-1.5 wt.% of agar.
Abstract translation: 提供一种从耕地分离土壤微生物的方法，通过将微生物的复杂培养物喷洒到耕地上，从耕地中分离具有优良土壤亲和力的微生物，从而提高环境友好型农产品的生产力， 从而促进植物生长。 从耕地分离土壤微生物的方法包括以下步骤：（a）将用于培养植物的土地土壤样品与盐溶液混合; （b）将相同土地的土壤样品添加到最小培养基中，以制备用于分离土壤友好微生物的最小培养基; （c）将从步骤（b）获得的混合物接种到由步骤（b）制备的最小培养基中; （d）在25-30℃的温度下培养由步骤（c）获得的接种溶液15-20天; （e）在用盐水溶液稀释步骤（d）得到的培养液后，用稀释液涂抹培养基进行分离，并在25-30℃的温度下培养5-7天; 和（f）分离培养基上形成的菌落作为相应土地的土壤友好的微生物，其中用于分离的最小培养基包括0.1-0.2重量/体积％的NH 4 Cl，0.44-0.54重量/体积％的K 2 HPO 4，0.4 -0.5重量/体积％的NaH 2 PO 4·2H 2 O，0.05-0.06重量/体积％的MgSO 4·7H 2 O，0.0003-0.0004重量/体积％的CaCl 2·2H 2 O，0.0001-0.0002重量/体积％的FeSO 4·7H 2 O，0.0001-0.0002 重量/体积％的MnCl 2·4H 2 O，0.00001-0.00002重量/体积％的CoCl 2·6H 2 O，0.00001-0.00002重量/体积％的Na 2 MoO 4·2H 2 O和0.0000重量/体积％的一水合柠檬酸和用于分离的培养基 包括0.4-0.5重量％的胨，0.2-0.3重量％的牛肉提取物和1.0-1.5重量％的琼脂。
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公开(公告)号：KR100768478B1
公开(公告)日：2007-10-19
申请号：KR1020060052186
申请日：2006-06-09
Applicant: 동국대학교 산학협력단
IPC: A01N61/00
Abstract: A plant anthracnose controlling composition comprising a natural mineral as an effective ingredient is provided to control the anthracnose in effective and environmental-friendly method, promote the growth of crops and restore contaminated soil. A composition for controlling anthracnose comprises a mixture consisting of 40.0-60.0 wt.% of SiO2, 10.0-25.0 wt.% of Al2O3, 10-25 wt.% of Fe2O3, 0.1-8.0 wt.5 of CaO, 0.1-8.0 wt.% of MgO, 0.5-7.0 wt.% of K2O, 0.1-5.0 wt.5 of Na2O, 0.1-5.0 wt.% of TiO2, and 0.01-2.0 wt.% of P2O5 or a natural mineral including the above ingredients, wherein a particle size of the natural mineral powder is 10-100,000 mesh. The anthracnose is controlled by treating the composition with a plant in need thereof.
Abstract translation: 提供包含天然矿物作为有效成分的植物炭疽病控制组合物，以有效和环保的方法控制炭疽病，促进作物生长并恢复受污染的土壤。 用于控制炭疽病的组合物包括由40.0-60.0重量％的SiO 2，10.0-25.0重量％的Al 2 O 3，10-25重量％的Fe 2 O 3，0.1-8.0重量％的CaO，0.1-8.0重量％ 的MgO，0.5-7.0重量％的K 2 O，0.1-5.0重量％的Na 2 O，0.1-5.0重量％的TiO 2和0.01-2.0重量％的P 2 O 5或包含上述成分的天然矿物， 其中所述天然矿物粉末的粒度为10-100,000目。 通过用需要的植物处理组合物来控制炭疽病。
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公开(公告)号：KR1020070118044A
公开(公告)日：2007-12-13
申请号：KR1020070056146
申请日：2007-06-08
Applicant: 동국대학교 산학협력단
Abstract: A method for improving plant growth is provided to promote growth of various plants without environmental pollution by using the self-circulation system of soil-favorable microorganisms, so that it is useful for increasing productivity, improving agricultural technique for organic agriculture and restoring the polluted soil. A method for improving plant growth comprises the steps of: (1) culturing a soil sample collected from the land under cultivation in a medium containing the corresponding land under cultivation to isolate soil-favorable microorganisms; (2) preparing the complex culture of isolated soil-favorable microorganisms by culturing the isolated soil-favorable microorganisms in a medium containing 0.4-0.5% of peptone and 0.2-0.3% of meat juice at 25-30 deg.C and 150-200 rpm for 48-72 hours; (3) spraying the complex culture to the corresponding land under cultivation, wherein the step(1) is carried out by (i) mixing the soil sample with physiological saline, inoculating the mixture into a minimal medium containing 1-10% of corresponding land under cultivation and culturing it at 25-30 deg.C for 15-20 days, (ii) diluting the cultured medium with physiological saline, spreading the diluted solution on a separation medium and culturing it at 25-30 deg.C for 5-7 days, and (iii) isolating colonies grown on the medium as soil-favorable microorganisms.
Abstract translation: 提供一种提高植物生长的方法，通过利用土壤有利微生物的自循环系统，促进各种植物的生长，无环境污染，有利于提高生产力，改善有机农业的农业技术和恢复污染土壤 。 一种改善植物生长的方法包括以下步骤：（1）培养在栽培土地上收集的土壤样品，在含有相应耕种土地的培养基中分离土壤有利微生物; （2）通过在25-30℃和150-200℃培养含有0.4-0.5％蛋白胨和0.2-0.3％肉汁的培养基中分离的土壤有利微生物来制备分离的有利于土壤的微生物的复杂培养物 转速48-72小时; （3）将复合培养物喷洒到相应的栽培土地上，其中步骤（1）是通过（i）将土壤样品与生理盐水混合进行的，将混合物接种在含有1-10％相应土地的基本培养基中 在25-30℃下培养和培养15-20天，（ii）用生理盐水稀释培养的培养基，将稀释的溶液分散在分离培养基上，并在25-30℃下培养5- 7天，和（iii）分离在培养基上生长的菌落作为土壤有利的微生物。
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