公开(公告)号：KR100995835B1

公开(公告)日：2010-11-23

申请号：KR1020070133314

申请日：2007-12-18

Applicant: 동국대학교 산학협력단

Inventor： 박정극 ,  윤희훈 ,  정보영

IPC: C12N5/071 ,  C12N5/02 ,  C12N5/00

Abstract: 본 발명은 (1) 중간엽 줄기세포를 세포증식 배지에서 배양하는 단계; (2) 단계 (1)에서 배양된 세포를 세포 성장인자가 첨가된 혈청 배지에서 간세포로 유도시키는 단계; 및 (3) 후속하여 단계 (2)에서 유도된 세포를 온코스타틴 엠(Oncostatin M), 글루카곤, 니코틴 아마이드 및 다이메틸 설폭사이드(dimethyl sulfoxide, DMSO)로부터 선택된 하나 이상의 간세포 성숙 유도 물질이 첨가된 혈청 배지에서 간세포를 성숙시키는 단계를 포함하는, 탯줄 유래의 중간엽 줄기세포 유래 간세포의 제조방법에 관한 것으로, 본 발명에 따른 방법으로 제조한 간세포는 정상적인 간세포와 유사한 알부민 합성능, 암모니아 제거능, 우레아 합성능, 싸이토케라틴 18(CK 18) 합성능, 싸이토크롬 P450 1B1 합성능 및 엑스비피-1(XBP-1) 합성능을 가짐으로써, 다양한 간세포 치료제, 체외순환형 생인공간 시스템 및 체외 독성평가 시스템의 세포원으로 이용할 수 있다.

公开(公告)号：KR1020090067601A

公开(公告)日：2009-06-25

申请号：KR1020070135308

申请日：2007-12-21

Applicant: 동국대학교 산학협력단

Inventor： 박정극 ,  윤희훈 ,  정보영

IPC: C12N5/02 ,  C12N5/00

Abstract: A method for producing a hepatocyte which is derived from an amnion epidermal cell is provided to treat liver diseases through the transplantation of cell and be used as a hepatocyte source for toxicity evaluation. A method for producing a hepatocyte which is derived from an amnion epidermal cell having the functions of albumin synthesis, urea synthesis, and ammonia removement comprises: a step of preparing amnion epidermal cell; a step of culturing the amnion epidermal cell in medium; and a step of differentiating the amnion epidermal cell to hepatocyte in serum medium in which a HGF (hepatocyte growth factor) is added. The serum medium contains mixture medium having DMEM(Dulbecco's Modified Eagle's Medium) and F-12 in a ratio of 3:1, fetal bovine serum, L-glutamine, hydrocortisone, insulin, transferring, epidermal growth factor, cholera toxin, triiodotyronine, and glucagon.

Abstract translation: 提供从羊膜表皮细胞产生肝细胞的方法，通过移植细胞来治疗肝脏疾病，并用作肝细胞来进行毒性评估。 一种产生具有白蛋白合成，尿素合成和氨移除功能的羊膜表皮细胞的肝细胞的制造方法，其特征在于，包括：制备羊膜表皮细胞的步骤; 在培养基中培养羊膜表皮细胞的步骤; 以及在其中加入HGF（肝细胞生长因子）的血清培养基中将羊膜表皮细胞分化为肝细胞的步骤。 血清培养基含有具有DMEM（Dulbecco's Modified Eagle's Medium）和3:1比例F-12，胎牛血清，L-谷氨酰胺，氢化可的松，胰岛素，转移，表皮生长因子，霍乱毒素，三碘青ron碱和 胰高血糖素。

公开(公告)号：KR1020090065814A

公开(公告)日：2009-06-23

申请号：KR1020070133314

申请日：2007-12-18

Applicant: 동국대학교 산학협력단

Inventor： 박정극 ,  윤희훈 ,  정보영

IPC: C12N5/071 ,  C12N5/02 ,  C12N5/00

Abstract: A method for manufacturing mesenchymal stem cell derived hepatocyte is provided to differentiate a mesenchymal stem cell which is derived from a wharton's jelly. A method for manufacturing mesenchymal stem cell derived hepatocyte comprises: a step of culturing the mesenchymal stem cell in cell proliferation medium; a step of inducing cells to hepatocyte in serum medium in which cell growth factor is added; and a step of maturing hepatocyte in serum medium in which a hepatocyte maturing induction material is added. The hepatocyte maturing induction material is selected from an oncostatin M, glucagon, nicotin amide and dimethyl sulfoxid(DMSO).

Abstract translation: 提供了间充质干细胞来源的肝细胞的制备方法，以区分来源于wharton果冻的间充质干细胞。 一种间充质干细胞衍生肝细胞的制备方法，包括：培养细胞增殖培养基中间充质干细胞的步骤; 在其中添加细胞生长因子的血清培养基中诱导细胞到肝细胞的步骤; 以及在添加了肝细胞成熟诱导材料的血清培养基中成熟肝细胞的步骤。 肝细胞成熟诱导材料选自制瘤素M，胰高血糖素，烟酰胺和二甲基亚砜（DMSO）。