Abstract:
PURPOSE: Separation and production methods of caffeine, epigallocatechin, epicatechin, epigallocatechingallate, and epicatechingallate from tea leaves are provided, thereby simultaneously separating and producing the five kinds of materials. CONSTITUTION: Separation and production methods of caffeine, epigallocatechin, epicatechin, epigallocatechingallate, and epicatechingallate from tea leaves comprises the steps of: steaming tea leaves and drying them; heat-extracting the dried tea leaves or extracting the extract thereof with EtOH and freeze-drying it; subjecting the powdery extract to the sephadex column to obtain fractions of caffeine, epigallocatechin, epicatechin, epigallocatechingallate, and epicatechingallate; and subjecting the fractions to HPLC, removing an organic solvent and freeze-drying the HPLC fractions.
Abstract:
PURPOSE: Provided are a Rubus coreanum fruit juice composition which has antioxidative function and contains, as natural antioxidants, 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, and 3,4-dihydroxycinnamic acid and a preparation method thereof. CONSTITUTION: A Rubus coreanum fruit juice composition having antioxidative function is characterized by containing, as natural antioxidative matters, 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, and 3,4-dihydroxycinnamic acid which have radical scavenging activity and antioxidative function.