公开(公告)号：KR1020010003889A

公开(公告)日：2001-01-15

申请号：KR1019990024378

申请日：1999-06-22

Applicant: 박근형

Inventor： 박근형 ,  임철근 ,  정순택

IPC: A01N65/08

CPC classification number: A01N65/08 ,  A01N31/08 ,  A01N37/10

Abstract: PURPOSE: A preparation method of gardenia fruit chrysophyll extract having inhibition activity of growth of microorganism such as Staphylococcus aureus, E. coli, and containing 3,4-dihydroxybenzoic acid is provided. CONSTITUTION: A preparation method of gardenia fruit chrysophyll extract is characterized by comprising the following steps: (i)grinding the dried gardenia fruit; (ii)washing by dipping in hexane solvent at room temperature for 24 hours so as to remove impurity and fat soluble component; (iii)after dipping residue in water, ethanol, methanol solvent, filtrating to obtain extract; (iv)swelling extract with water or ethanol or methanol solvent by using Sephadex LH-20 and eluting and fractionizing; (v)purifying and manufacturing gardenia fruit chrysophyll extract by collecting eluate fraction whose Ve/Vt(ratio of elution volume to total column volume) is 0.8-1.2; (vi)performing vacuum concentration or freeze drying.

Abstract translation: 目的：提供具有抑制金黄色葡萄球菌，大肠杆菌，含有3,4-二羟基苯甲酸等微生物生长抑制活性的栀子果汁提取物的制备方法。 构成：栀子果汁提取物的制备方法的特征在于包括以下步骤：（i）研磨干栀子果实; （ii）通过在己烷溶剂中浸渍在室温下洗涤24小时，以除去杂质和脂溶性组分; （iii）将残留物浸在水，乙醇，甲醇溶剂中，过滤得到提取物; （iv）使用Sephadex LH-20用水或乙醇或甲醇溶剂溶胀提取物，洗脱分级; （v）通过收集Ve / Vt（洗脱体积与总柱体积的比例）为0.8-1.2的洗脱液级分来纯化和制备栀子果汁黄酮提取物; （vi）进行真空浓缩或冷冻干燥。

公开(公告)号：KR100304005B1

公开(公告)日：2001-09-24

申请号：KR1019990024378

申请日：1999-06-22

Applicant: 박근형

Inventor： 박근형 ,  임철근 ,  정순택

IPC: A01N65/08

Abstract: 본발명은 3,4-디히드록시벤조산을포함한미생물생육저해기능을갖는치자열매황색소추출물의제조에관한것으로, 치자열매를마쇄한후 용매로추출한다음여과하여얻어진황색소추출물은미생물에생육저해기능을가진천연색소로, 추출물에함유된활성물질은 HPLC 등으로분리하여 MS, NMR 등으로분석한결과 3,4-디히드록시벤조산으로동정된물질을포함하며, 이추출물은미생물생육저해기능을갖는치자열매황색소추출물로식품첨가제, 공예품의염료, 의복의착색제등에유용하게이용될수 있다.