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公开(公告)号：KR1020010077323A
公开(公告)日：2001-08-17
申请号：KR1020000005033
申请日：2000-02-02
Applicant: 박근형
IPC: A23F3/00
Abstract: PURPOSE: A method for preparing a tea using the trunk and leaves of Hovenia dulcis and preparing a granule tea and a drink using their hot-water extract is provided. Thereby, the trunk and leaves of Hovenia dulcis is utilized well, which has fluent minerals, nutrition, effects to remove alcohol poisoning, and antibacterial and antioxidant effects. CONSTITUTION: A method for preparing a tea using the trunk and leaves of Hovenia dulcis comprises the steps of: (i) collecting the trunk and leaves of Hovenia dulcis from middle June to late July; (ii) heating with hot steam of 0.2kg/cm¬2 and 95deg.C for 20-45 minutes to inactivate the enzyme of leaves and trunk; (iii) cooling with cool air; (iv) destructing the leaves and trunk and drawing out water from them with a related device; (v) drying with a hot air dryer at 50-60deg.C for 10hours, and then pulverizing in 2mm size or less; and (vi) packing in tea bags. The prepared tea can be made into a granule tea and a drink using its hot-water extract.
Abstract translation: 目的：提供使用枸杞子的树干和叶子制备茶的方法，并使用其热水提取物制备颗粒茶和饮料。 因此，枸杞子的树干和叶片利用效果好，具有流动的矿物质，营养，去除酒精中毒的作用，抗菌和抗氧化作用。 构成：使用枸杞子的树干和叶子制备茶的方法包括以下步骤：（i）从6月中旬至7月下旬收集枸杞子的树干和叶子; （ii）用0.2kg / cm 2和95℃的热蒸汽加热20-45分钟以灭活叶和树干的酶; （iii）用冷空气冷却; （iv）破坏叶子和树干，用相关装置从中抽出水; （v）用50-60℃的热空气干燥器干燥10小时，然后粉碎至2mm或更小; 和（vi）包装在茶包中。 使用其热水提取物制成的茶可制成颗粒茶和饮料。
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Patent Agency Ranking
公开(公告)号：KR1020020043131A
公开(公告)日：2002-06-08
申请号：KR1020000072613
申请日：2000-12-01
Applicant: 박근형
IPC: C07D213/62
Abstract: PURPOSE: A novel natural antimicrobial substance isolated from the stalk and leaf of Hovenia dulcis Thunb and an isolating method thereof are provided. Therefore, the novel natural antimicrobial substance, hovenidine B6, having pyridine structure can be found. CONSTITUTION: The novel natural antimicrobial substance, hovenidine B6 is isolated from the stalk and leaf of Hovenia dulcis Thunb by the steps of: heat-extracting the stalk and leaf of Hovenia dulcis Thunb; centrifuging the extract and freeze-drying the supernatant to obtain the extract powder; concentrating the methanol filtrate under reduced pressure to obtain methanol extract; adding a weak alkali buffer and ethylacetate into the methanol extract to divide a liquid fraction and an ethylacetate neutral fraction, and adding ethylacetate into the liquid fraction to obtain ethylacetate acid fraction; subjecting the above fractions to silica gel adsorption column chromatography using ethylacetate-methanol solution to obtain an active fraction; subjecting the active fraction to gel filtration chromatography using Sephadex LH-20 column; and subjecting the purified fraction to reverse HPLC using methanol-water solvent to isolate hovenidine B6.
Abstract translation: 目的：提供从枸杞子的茎和叶分离的新型天然抗菌物质及其分离方法。 因此，可以发现具有吡啶结构的新型天然抗菌物质赫非啶B6。 构成：通过以下步骤从枸杞子的茎和叶中分离出新颖的天然抗菌物质贝伐单菌B6：热提取枸杞子的茎和叶; 离心提取物并冷冻干燥上清液，得到提取物粉末; 减压浓缩甲醇滤液得甲醇萃取液; 在甲醇提取液中加入弱碱性缓冲液和乙酸乙酯，分离出液体馏分和乙酸乙酯中性馏分，并向乙酸乙酯中加入乙酸乙酯，得到乙酸乙酯酸馏分; 将上述级分用乙酸乙酯 - 甲醇溶液进行硅胶吸附柱层析，得到活性部分; 使用Sephadex LH-20柱对活性部分进行凝胶过滤色谱; 并使用甲醇 - 水溶剂对纯化的级分进行反相HPLC，以分离出霍尼定B6。
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3.发明授权
公开(公告)号：KR100439012B1
公开(公告)日：2004-07-03
申请号：KR1020010017769
申请日：2001-03-29
Applicant: 박근형
Abstract: PURPOSE: A natural antioxidant substance containing 4-hydroxy-3-methoxy benzoic acid, 4-hydroxy-3-methoxy cinnamic acid, quercetin-3-0-β-D-glucopyranoside and hovenidine B6 as a new natural antioxidant active material separated from Hovenia dulcis Thunb. is provided which is expected to be used as a material of food and medicine having an anti-aging function. CONSTITUTION: Leaves and stems of Hovenia dulcis Thunb. are extracted in water, freeze dried and then extracted in methanol, followed by solvent fractionation to produce an ethyl acetate acidic fraction and neural fraction. An active material contained in the extract is then purified with a purifying means such as silica gel column chromatography, chromatography with Sephadex LH-20, octadecylsilane column chromatography, a reversed-phase high-performance liquid chromatography or the like.
Abstract translation: 用途：含有4-羟基-3-甲氧基苯甲酸，4-羟基-3-甲氧基肉桂酸，槲皮素-3-O-β-D-吡喃葡萄糖苷和hovenidine B6作为新的天然抗氧化剂活性物质的天然抗氧化剂物质 从Hovenia dulcis Thunb分离的材料。 预计将被用作具有抗衰老功能的食物和药物的材料。 组成：Hovenia dulcis Thunb的叶和茎。 在水中萃取，冷冻干燥，然后用甲醇萃取，随后溶剂分馏以产生乙酸乙酯酸性部分和神经部分。 然后提取物中所含的活性物质用硅胶柱色谱等纯化手段，Sephadex LH-20色谱法，十八烷基硅烷柱色谱法，反相高效液相色谱法等纯化。
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4.发明公开
公开(公告)号：KR1020020076980A
公开(公告)日：2002-10-11
申请号：KR1020010017769
申请日：2001-03-29
Applicant: 박근형
Abstract: PURPOSE: A natural antioxidant substance containing 4-hydroxy-3-methoxy benzoic acid, 4-hydroxy-3-methoxy cinnamic acid, quercetin-3-0-β-D-glucopyranoside and hovenidine B6 as a new natural antioxidant active material separated from Hovenia dulcis Thunb. is provided which is expected to be used as a material of food and medicine having an anti-aging function. CONSTITUTION: Leaves and stems of Hovenia dulcis Thunb. are extracted in water, freeze dried and then extracted in methanol, followed by solvent fractionation to produce an ethyl acetate acidic fraction and neural fraction. An active material contained in the extract is then purified with a purifying means such as silica gel column chromatography, chromatography with Sephadex LH-20, octadecylsilane column chromatography, a reversed-phase high-performance liquid chromatography or the like.
Abstract translation: 目的：一种天然的抗氧化物质，含有4-羟基-3-甲氧基苯甲酸，4-羟基-3-甲氧基肉桂酸，槲皮素-3-O-β-D-吡喃葡萄糖苷， Ho ia is 其被预期用作具有抗衰老功能的食品和药物的材料。 构成：枸杞子的叶子和茎。 在水中萃取，冷冻干燥，然后在甲醇中萃取，随后进行溶剂分馏，得到乙酸乙酯酸性馏分和神经级分。 然后用纯化方法如硅胶柱色谱，Sephadex LH-20色谱法，十八烷基硅烷柱色谱法，反相高效液相色谱法等纯化提取物中所含的活性物质。
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5.发明公开쿼서틴 3-O-α-L-람노시드, 캠페롤3-O-α-L-람노피라노시드, 캠페롤 3,7-O-α-L-디람노시드,카페인, 캠페롤3-O-α-L-람노실(1→6)-O-β-D-글루코실(1→2)-O-β-D-글루코시드, 그리고 캠페롤3-O-α-L-람노시드-7-O-[β-D-글루코실-(1→3)-α-L-람노시드] 등의 천연항산화물질을 포함하여 항산화 기능을 갖는헛개나무 추출물 无效提取含有3-O-α-L-赖氨酸奎烷酮，3-O-α-L-赖氨酸，3,7- O-α-L-吡喃半乳糖苷，CAFFEINE，KAEMPFEROL 3抗坏血酸的抗氧化功能的制剂， （1→6）-O-β-D-吡喃葡萄糖苷（1→2）-O-β-D-葡糖苷和3-O-α-L-降冰片烯-7- O- [β-D-葡糖基-L-（1-> 3）-alpha- L-赖氨酸]
公开(公告)号：KR1020050105910A
公开(公告)日：2005-11-08
申请号：KR1020040032030
申请日：2004-05-03
Applicant: 박근형
IPC: A61K31/7048 , A61K31/522 , A61K36/13
CPC classification number: A61K31/7048 , A61K31/522 , A61K36/72 , A61K2236/33 , A61K2236/51
Abstract: 헛개나무(
Hovenia dulcis Thunb) 줄기와 잎의 열수추출물 및 에탄올추출물에서 쿼서틴 3-
O -α- L-람노시드, 캠페롤 3-
O -α- L-람노피라노시드, 캠페롤 3,7-
O -α- L-디람노시드, 카페인, 캠페롤 3-
O -α- L-람노실(1→6)-
O -β- D-글루코실(1→2)-
O -β- D-글루코시드, 그리고 캠페롤 3-
O -α- L-람노시드-7-
O -[β- D-글루코실-(1→3)-α- L-람노시드] 등의 천연항산화 활성물질이 존재함을 발견하였다. 즉, 헛개나무의 줄기와 잎을 열수로 추출하여 얻어진 열수추출물을 동결건조한 다음, 메탄올로 용해하여 얻어진 메탄올 가용 추출물을 다양한 컬럼 크로마토그라피 및 HPLC 등의 단계를 거쳐 쿼서틴 3-
O -α- L-람노시드 및 캠페롤 3-
O -α- L-람노시드를 분리하였다. 또 헛개나무 줄기와 잎의 에탄올 추출물을 용매분획하여 얻어진 부탄올 추출물로부터 캠페롤 3,7-
O -a- L-디람노시드, 카페인, 캠페롤 3-
O -α- L-람노실(1→6)-
O -β- D-글루코실(1→2)-
O -β- D-글루코시드, 그리고 캠페롤 3-
O -α- L-람노시드-7-
O -[β- D-글루코실-(1→3)-α- L-람노시드]를 분리하였다. 분리된 물질의 구조결정은 MS, NMR 분석에 의해 이루어졌으며, 분리된 물질은 항산화 활성을 갖고 있음이 확인되었다. 천연항산화 물질을 포함하는 헛개나무 줄기와 잎의 추출물은 산화에 의한 질병예방 및 노화억제 효력을 갖는 기능성 소재로서의 용도를 제공한다.-
公开(公告)号：KR100388357B1
公开(公告)日：2003-06-19
申请号：KR1020000072613
申请日：2000-12-01
Applicant: 박근형
IPC: C07D213/62
Abstract: PURPOSE: A novel natural antimicrobial substance isolated from the stalk and leaf of Hovenia dulcis Thunb and an isolating method thereof are provided. Therefore, the novel natural antimicrobial substance, hovenidine B6, having pyridine structure can be found. CONSTITUTION: The novel natural antimicrobial substance, hovenidine B6 is isolated from the stalk and leaf of Hovenia dulcis Thunb by the steps of: heat-extracting the stalk and leaf of Hovenia dulcis Thunb; centrifuging the extract and freeze-drying the supernatant to obtain the extract powder; concentrating the methanol filtrate under reduced pressure to obtain methanol extract; adding a weak alkali buffer and ethylacetate into the methanol extract to divide a liquid fraction and an ethylacetate neutral fraction, and adding ethylacetate into the liquid fraction to obtain ethylacetate acid fraction; subjecting the above fractions to silica gel adsorption column chromatography using ethylacetate-methanol solution to obtain an active fraction; subjecting the active fraction to gel filtration chromatography using Sephadex LH-20 column; and subjecting the purified fraction to reverse HPLC using methanol-water solvent to isolate hovenidine B6.
Abstract translation: 用途：提供一种从枳st leaf和叶中分离出的新型天然抗菌物质及其分离方法。 因此，可以发现具有吡啶结构的新型天然抗微生物物质hovenidine B6。 本发明的新颖天然抗菌物质Hovenidine B6是通过以下步骤从枳st st和叶中分离出来的：热榨枳st st和叶; 离心提取物并冷冻干燥上清液以获得提取物粉末; 减压浓缩甲醇滤液得到甲醇提取物; 向甲醇提取液中加入弱碱缓冲液和乙酸乙酯，分液体部分和乙酸乙酯中性部分，向液体部分中加入乙酸乙酯，得到乙酸乙酯酸部分; 使用乙酸乙酯 - 甲醇溶液将上述级分进行硅胶吸附柱色谱法以获得活性级分; 用Sephadex LH-20柱对活性部分进行凝胶过滤层析; 并使用甲醇 - 水溶剂使纯化级分进行反相HPLC以分离Hovenidine B6。
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