公开(公告)号：KR1020030087868A

公开(公告)日：2003-11-15

申请号：KR1020020025951

申请日：2002-05-10

Applicant: 박연일

Inventor： 류지연 ,  김수연 ,  박영목 ,  이영하 ,  박연일

IPC: C12N1/12

Abstract: PURPOSE: A gene-defective Cyanobacteria, and methods for increasing salt resistance of plants and productivity of PHB(poly-beta-hydroxybutyrate) using the same are provided. The productivity of plants is improved by minimizing damage by salts. CONSTITUTION: Cta1 gene-defective Cyanobacteria, Synechocystis sp. PCC6083 Δ Cta1 (KCTC 10247BP) is provided, wherein Synechocystis sp. PCC6083 ΔCta1 (KCTC 10247BP) is obtained by isolating Cta1 gene, damaging the isolated Cta1 gene, inserting the damaged Cta1 gene into a vector to construct a plasmid pGEM Z, and transforming Synechocystis sp. PCC6083 with pGEM Z using humongous recombination. A method for increasing productivity of PHB(poly-beta-hydroxybutyrate) comprises culturing Synechocystis sp. PCC6083 ΔCta1 (KCTC 10247BP) in a medium.

Abstract translation: 目的：提供一种基因缺陷型蓝细菌，以及提高植物耐盐性和使用该植物的PHB（聚-β-羟基丁酸酯）的生产率的方法。 通过最小化盐的损害来改善植物的生产力。 构成：Cta1基因缺陷蓝藻，集胞藻 提供PCC6083ΔCta1（KCTC 10247BP），其中集胞藻 PCC6083ΔCta1（KCTC 10247BP）通过分离Cta1基因，破坏分离的Cta1基因，将损伤的Cta1基因插入载体中构建质粒pGEM Z，并转化集胞藻（Synechocystis sp。 PCC6083与pGEM Z使用强大的重组。 提高PHB（聚-β-羟基丁酸酯）生产率的方法包括培养集胞藻（Synechocystis sp。 PCC6083ΔCta1（KCTC 10247BP）在培养基中。