公开(公告)号：KR1020130084380A

公开(公告)日：2013-07-25

申请号：KR1020120005106

申请日：2012-01-17

Applicant: 서강대학교산학협력단

Inventor： 신운섭 ,  권덕

IPC: H01M8/16 ,  H01M4/86 ,  G01N33/68 ,  C12Q1/26

CPC classification number: Y02E60/527 ,  Y02P70/56

Abstract: PURPOSE: A high efficiency oxidation-reduction active electrode is provided to show excellent stability, and to use as oxidation-reduction platform of electrochemical reaction by its own electrochemical characteristic. CONSTITUTION: An oxidation-reduction active electrode comprises: an electrode, and a membrane formed on the surface of the electrode, comprising: an ion-exchange resin reformed as an oxidation-reduction intermediary which has a sulfone radical on the branched chain; and a hydrophilic polyurethane. A manufacturing method of the oxidation-reduction active electrode comprises the steps of: sintering the ion-exchange resin with the oxidation-reduction intermediary; and forming the hydrophilic polyurethane membrane containing the reformed ion-exchange resin on the surface of the electrode. The ion-exchange resin is perfluorinated cation exchange resin equipped with a channel structure, in which the ion-exchange reaction occurs by the sulfone radical positioned on the inner wall of the channel.

Abstract translation: 目的：提供高效氧化还原活性电极，以显示出优异的稳定性，并通过其自身的电化学特性用作电化学反应的氧化还原反应平台。 构成：氧化还原活性电极包括：电极和形成在电极表面上的膜，包括：作为在支链上具有砜基的氧化还原中间体重整的离子交换树脂; 和亲水性聚氨酯。 氧化还原活性电极的制造方法包括以下步骤：用氧化还原中间体烧结离子交换树脂; 并在电极表面上形成含有重整离子交换树脂的亲水性聚氨酯膜。 离子交换树脂是配备有通道结构的全氟化阳离子交换树脂，其中通过位于通道内壁上的砜基发生离子交换反应。

公开(公告)号：KR1020130084377A

公开(公告)日：2013-07-25

申请号：KR1020120005099

申请日：2012-01-17

Applicant: 서강대학교산학협력단

Inventor： 신운섭 ,  권덕

IPC: G01N33/68 ,  C12Q1/26 ,  G01N33/535 ,  G01N33/543

Abstract: PURPOSE: An enzyme fixation method is provided to fix biocatalysts such as an enzyme on the surface of an electrode by simple and effective electrodeposition (ED) and to have long-term enzyme activity and stability on the surface of the electrode. CONSTITUTION: An enzyme fixation method comprises the steps of: applying predetermined applied voltage to a mixture solution containing chitosan, an electron carrier, an enzyme, and a buffer solution; and electrodeposition by forming a complex layer of the chitosan and the enzyme on an electrode. The method also comprises the step of fixing the complex layer through adsorption by providing a predetermined quiet time before electrodeposition. An enzyme electrode is prepared by fixing the enzyme on the electrode and comprises the complex layer fixed in a thickness of 100-1000 nm. A biosensor contains the enzyme electrode on the electrode.

Abstract translation: 目的：提供酶固定方法，通过简单有效的电沉积（ED）将生物催化剂如酶固定在电极表面，并具有长期的酶活性和电极表面的稳定性。 构成：酶固定方法包括以下步骤：将预定的施加电压施加到含有壳聚糖，电子载体，酶和缓冲溶液的混合溶液中; 并通过在电极上形成壳聚糖和酶的复合层进行电沉积。 该方法还包括通过在电沉积之前提供预定的安静时间来固定复合层通过吸附的步骤。 通过将酶固定在电极上并包含固定在100-1000nm厚度的复合层来制备酶电极。 生物传感器在电极上含有酶电极。