公开(公告)号：KR101542879B1

公开(公告)日：2015-08-10

申请号：KR1020130083817

申请日：2013-07-16

Applicant: 서강대학교산학협력단

Inventor： 이진원 ,  오민규 ,  정무영

IPC: C12N1/21 ,  C12N15/53 ,  C12P7/16

CPC classification number: C12P7/18 ,  C07K14/245 ,  C07K14/26 ,  C12N9/0006 ,  C12N15/00 ,  C12Y101/01027

Abstract: 본발명은 (a) 불활성화(inactivated) 락테이트디하이드로제나제(lactate dehydrogenase) 및 (b) 불활성화(inactivated) 수크로즈조절자(sucrose regulator)를포함하는 2,3-부탄다이올생성용재조합균주에관한것이다. 본발명에따르면, 본발명은저렴한탄소원을이용하여경제적으로 2,3-부탄아이올을생산할수 있고, 야생형과비교하여우수한 2,3-부탄다이올효율및 생산성을나타낸다.

公开(公告)号：KR1020140095945A

公开(公告)日：2014-08-04

申请号：KR1020130083817

申请日：2013-07-16

Applicant: 서강대학교산학협력단

Inventor： 이진원 ,  오민규 ,  정무영

IPC: C12N1/21 ,  C12N15/53 ,  C12P7/16

CPC classification number: C12P7/18 ,  C07K14/245 ,  C07K14/26 ,  C12N9/0006 ,  C12N15/00 ,  C12Y101/01027

Abstract: The present invention relates to a recombinant strain producing 2,3-butanediol which comprises (a) an inactivated lactate dehydrogenase and (b) an inactivated sucrose regulator. According to the present invention, 2,3-butanediol is economically produced using a cheap carbon source in the present invention, and excellent 2,3-butanediol efficiency and productivity are obtained compared with a wild type.

Abstract translation: 本发明涉及产生2,3-丁二醇的重组菌株，其包含（a）灭活的乳酸脱氢酶和（b）灭活的蔗糖调节剂。 根据本发明，与本发明相比，本发明中使用廉价碳源经济地制造2,3-丁二醇，与野生型相比，获得了优异的2,3-丁二醇效率和生产率。

公开(公告)号：KR101351989B1

公开(公告)日：2014-01-27

申请号：KR1020120015593

申请日：2012-02-16

Applicant: 서강대학교산학협력단

Inventor： 이진원 ,  오민규 ,  정무영 ,  황천유

IPC: C12N1/19 ,  C12N15/53 ,  C12P7/16

Abstract: 본 발명은 알코올 디히드로제나아제(alcohol dehydrogenase) 1, 알코올디히드로제나아제 3 및 알코올 디히드로제나아제 5로 구성된 군으로부터 선택되는 하나이상의 알코올 디히드로제나아제를 불활성화시키는 단계를 포함하는 2,3-부탄다이올 제조용 효모의 제조방법에 관한 것이다. 본 발명은 화학공업에 유용한 기초가 될 플랫폼용 화합물인 2,3-부탄다이올 제조용 효모 제조방법, 2,3-부탄다이올 제조용 효모 및 2,3-부탄다이올을 제공할 수 있으며, 본 발명의 2,3-부탄다이올 제조용 효모는 야생형 균주와 비교하여 50-100배 향상된 우수한 2,3-부탄다이올 효율을 나타낸다.

公开(公告)号：KR1020130094394A

公开(公告)日：2013-08-26

申请号：KR1020120015593

申请日：2012-02-16

Applicant: 서강대학교산학협력단

Inventor： 이진원 ,  오민규 ,  정무영 ,  황천유

IPC: C12N1/19 ,  C12N15/53 ,  C12P7/16

Abstract: PURPOSE: A method for preparing yeast for producing 2,3-butanediol is provided to produce 50 to 100 times more 2,3-butanediol than a wild strain. CONSTITUTION: A method for preparing yeast for producing 2,3-butanediol comprises the step of inactivating one or more kinds of alcohol dehydrogenase selected from among alcohol dehydrogenase 1, alcohol dehydrogenase 3, and alcohol dehydrogenase 5. The step is conducted by mutating a nucleotide sequence for coding alcohol dehydrogenase through substitution, insertion, deletion, or a combination thereof. The efficiency of the yeast for producing 2,3-butanediol is 50 to 100 times greater than that of a wild strain of yeast.

Abstract translation: 目的：提供制备2,3-丁二醇的酵母的方法，以生产比野生菌株多出50-100倍的2,3-丁二醇。 构成：制备2,3-丁二醇的酵母的方法包括使选自醇脱氢酶1，醇脱氢酶3和醇脱氢酶5中的一种或多种醇脱氢酶失活的步骤。该步骤是通过使核苷酸 通过取代，插入，缺失或其组合来编码醇脱氢酶的序列。 用于生产2,3-丁二醇的酵母的效率比酵母野生株的效率高50-100倍。

公开(公告)号：KR1020120104765A

公开(公告)日：2012-09-24

申请号：KR1020110022372

申请日：2011-03-14

Applicant: 서강대학교산학협력단 ,  고려대학교 산학협력단

Inventor： 이진원 ,  오민규 ,  정무영 ,  황천유

IPC: C12N1/21 ,  C12N15/74 ,  C12N15/31 ,  C12P7/16

Abstract: PURPOSE: A lactate dehydrogenase gene-deleted Enterobacter aerogenes is provided to highly prepare 2,3-butandiol and to be used as alternative energy. CONSTITUTION: A lactate dehydrogenase gene-deleted Enterobacter aerogenes has improved 2,3-butandiol productivity. A vector for removing the gene contains flanking sequence of lactate dehydrogenase gene and FRT-kanamyucin-FRT cassette. The vector is applied to the Enterobacter aerogenes. A method for preparing the Enterobacter aerogenes comprises: a step of transforming the vector to Enterobacter aerogenes to remove the lactate dehydrogenase gene by electroporation; and a step of selecting lactate dehydrogenase gene-deleted Enterobacter aerogenes.

Abstract translation: 目的：提供乳酸脱氢酶基因缺失的产气肠杆菌高度制备2,3-丁二醇并用作替代能量。 构成：乳酸脱氢酶基因缺失的产气肠杆菌具有改善的2,3-丁二醇生产力。 用于除去该基因的载体含有乳酸脱氢酶基因和FRT-卡纳米霉素-FRT盒的侧翼序列。 将载体应用于产气肠杆菌。 制备产气肠杆菌的方法包括：将载体转化成产气肠杆菌以通过电穿孔除去乳酸脱氢酶基因的步骤; 以及选择乳酸脱氢酶基因缺失的产气肠杆菌的步骤。