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公开(公告)号：KR101174547B1
公开(公告)日：2012-08-16
申请号：KR1020100012239
申请日：2010-02-10
Applicant: 서울대학교산학협력단
Abstract: 본발명은 betaPix 유전자결핍생쥐로부터유래한불멸화된생쥐배아섬유아세포주(Mouse embryonic fibroblast cell line, MEF cell line) 및그의제조방법에관한것이다. 보다구체적으로본 발명은생쥐 betaPix 유전자결핍생쥐의배아로부터유래한섬유아세포를 SV40T 항원을포함하는플라스미드를이용하여불멸화시켜얻은 betaPix 유전자결핍생쥐배아섬유아세포주및 그의제조방법에관한것이다. 본발명의불멸화된 betaPix 유전자결핍생쥐배아섬유아세포주는다양한 betaPix 유전자군의기능을밝히는데 있어유용하게이용될수 있다.
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2.发明公开
公开(公告)号：KR1020110092681A
公开(公告)日：2011-08-18
申请号：KR1020100012239
申请日：2010-02-10
Applicant: 서울대학교산학협력단
Abstract: PURPOSE: An immortalized mouse embryonic fibroblast cell line derived from a betaPIX-knocked out mouse embryo is provided to study the effect of the gene on fibroblast migration. CONSTITUTION: A method for preparing an immortalized mouse embryonic fibroblast cell line comprises: a step of mating betaPIX gene-knocked out mice; a step of extracting an embryo; a step of isolating genome DNA from the yolk sac of the embryo and confirm by PCR; a step of isolating primary fibroblasts from the embryo and a step of culturing in a DMEM medium. The cell migration study is performed by wound healing assay.
Abstract translation: 目的：提供一种源自βPIX敲除小鼠胚胎的永生化小鼠胚胎成纤维细胞系，以研究该基因对成纤维细胞迁移的影响。 构成：一种制备永生化小鼠胚胎成纤维细胞系的方法，包括：将βPIX基因敲除小鼠交配的步骤; 提取胚胎的步骤; 从胚胎卵黄囊分离基因组DNA并通过PCR确认的步骤; 从胚胎中分离原代成纤维细胞的步骤和在DMEM培养基中培养的步骤。 通过伤口愈合实验进行细胞迁移研究。
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