탈세포화를 위한 돼지 각막의 가공방법
    2.
    发明公开
    탈세포화를 위한 돼지 각막의 가공방법 有权
    过程用于细分化的方法

    公开(公告)号:KR1020100099787A

    公开(公告)日:2010-09-15

    申请号:KR1020090018294

    申请日:2009-03-04

    Abstract: PURPOSE: A method for processing porcine cornea for decellularization is provided to use as a substrate in alternative cornea for in vitro culture and histotechonology. CONSTITUTION: A method for porcine cornea comprises: a step(a) for immersing extracted porcine cornea in a solution containing NaCl; a step of transferring the porcine cornea into a solution containing 0.01-0.10%(w/v) of trypsin and 0.01-0.05%(w/v) of EDTA; and a step of washing the porcine cornea. The concentration of the solution containing NaCl is 1.0-2.0 M.

    Abstract translation: 目的:提供一种处理猪角膜去细胞化的方法,用作替代角膜的底物,用于体外培养和组织学。 构成:猪角膜法包括:将提取的猪角膜浸入含有NaCl的溶液中的步骤(a) 将猪角膜转移到含有0.01-0.10%(w / v)胰蛋白酶和0.01-0.05%(w / v)EDTA的溶液中的步骤; 和洗涤猪角膜的步骤。 含有NaCl的溶液的浓度为1.0-2.0M。

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