公开(公告)号：KR1020100022866A

公开(公告)日：2010-03-03

申请号：KR1020080081578

申请日：2008-08-20

Applicant: 서울대학교산학협력단

Inventor： 박주철 ,  박종태 ,  김흥중 ,  손호현

IPC: A61K38/16 ,  C12Q1/68

CPC classification number: C12Q1/6886 ,  A61K38/16 ,  C12Q2600/112

Abstract: PURPOSE: A composition containing ODAM recombinant protein is provided to promote mineralization of enamel and evaluate expression level of the ODAM gene. CONSTITUTION: A composition for promoting mineralization of enamel contains an ODAM recombinant protein. A method for producing the ODAM recombinant protein comprises: a step of culturing E.coli(DE3) transformed with pLysS(pRSET-ODAM); a step of isolating a protein from E.coli(DE3); and a step of purifying ODAM protein using His-Tag protein through FPLC. A method for evaluating overexpression of ODAM gene comprises: a step of collecting ameloblast from teeth; a step of measuring mRNA expression level of gene of ODAM, MEF2, aurora kinase A, BMP receptor IB or EF-hand calcium binding protein, and GAPDH from the ameloblast; and a step of comparing mRNA expression level between the ameloblast and normal ameloblast based on mRNA expression level of GAPDH gene.

Abstract translation: 目的：提供含有ODAM重组蛋白的组合物，以促进釉质的矿化，并评估ODAM基因的表达水平。 构成：用于促进釉质矿化的组合物含有ODAM重组蛋白。 制备ODAM重组蛋白的方法包括：培养用pLysS（pRSET-ODAM）转化的大肠杆菌（DE3）的步骤; 从大肠杆菌（DE3）中分离蛋白质的步骤; 以及使用His-Tag蛋白通过FPLC纯化ODAM蛋白的步骤。 评估ODAM基因过表达的方法包括：从牙齿中收集成釉细胞的步骤; 测量ODAM，MEF2，极光激酶A，BMP受体IB或EF-手钙结合蛋白的基因的mRNA表达水平和来自成釉细胞的GAPDH的步骤; 基于GAPDH基因的mRNA表达水平，比较成釉细胞与正常成釉细胞之间的mRNA表达水平。