公开(公告)号：KR1020100102273A

公开(公告)日：2010-09-24

申请号：KR1020090020563

申请日：2009-03-11

Applicant: 서울대학교산학협력단

Inventor： 조명행 ,  이윤식 ,  정대홍 ,  우민아 ,  김성용 ,  이상명

IPC: G01N33/52 ,  G01N33/533

Abstract: PURPOSE: A qualitative method of a multiple marker is provided to performing qualitation of high sensitivity and selectivity using F-SERS dots. CONSTITUTION: A qualitative method for a multiple marker comprises: a step of treating multiple marker-specific fluorescence-surface enhanced raman scattering(SERS) dot mixture to a sample; and a step of measuring raman signal corresponding to the multiple marker to calculate relative amount of the multiple marker. The SERS dot comprises: a metal nanoparticle layer containing a metal on the surface of silica nanoparticle; a raman probe and fluorescence probe layer formed at the metal nanoparticle layer; a functional layer; and a reaction molecule attached on the functional layer. The diameter of the silica nanoparticle is 50-300nm. The metal is gold, silver, palladium, platinum, aluminum, iridium, osmium, rhodium or ruthenium. The functional group is thiol group(-SH), amine group(-NH_2) or carboxyl group(-COOH).

Abstract translation: 目的：提供多重标记的定性方法，以使用F-SERS点进行高灵敏度和选择性的定性。 构成：多重标记的定性方法包括：将多个标记特异性荧光表面增强拉曼散射（SERS）点混合物处理到样品的步骤; 以及测量与所述多个标记相对应的拉曼信号以计算所述多个标记的相对量的步骤。 SERS点包括：在二氧化硅纳米颗粒的表面上含有金属的金属纳米颗粒层; 在金属纳米颗粒层形成的拉曼探针和荧光探针层; 功能层; 和附着在功能层上的反应分子。 二氧化硅纳米颗粒的直径为50-300nm。 金属是金，银，钯，铂，铝，铱，锇，铑或钌。 官能团是硫醇基（-SH），胺基（-NH2）或羧基（-COOH）。